Multimodal Control of Bacterial Gene Expression by Red and Blue Light
acceptedVersion
5.0 Mb
Meier, S. S. M., Multamäki, E., Ranzani, A. T., Takala, H., & Möglich, A. (2024). Multimodal Control of Bacterial Gene Expression by Red and Blue Light. In J. C. Braman (Ed.), Synthetic Biology : Methods and Protocols (pp. 463-477). Humana Press. Methods in Molecular Biology, 2760. https://doi.org/10.1007/978-1-0716-3658-9_26
Julkaistu sarjassa
Methods in Molecular BiologyPäivämäärä
2024Pääsyrajoitukset
Tekijänoikeudet
© 2024 the Authors
By applying sensory photoreceptors, optogenetics realizes the light-dependent control of cellular events and state. Given reversibility, noninvasiveness, and exquisite spatiotemporal precision, optogenetic approaches enable innovative use cases in cell biology, synthetic biology, and biotechnology. In this chapter, we detail the implementation of the pREDusk, pREDawn, pCrepusculo, and pAurora optogenetic circuits for controlling bacterial gene expression by red and blue light, respectively. The protocols provided here guide the practical use and multiplexing of these circuits, thereby enabling graded protein production in bacteria at analytical and semi-preparative scales.
Julkaisija
Humana PressEmojulkaisun ISBN
978-1-0716-3657-2Kuuluu julkaisuun
Synthetic Biology : Methods and ProtocolsISSN Hae Julkaisufoorumista
1064-3745Asiasanat
Julkaisu tutkimustietojärjestelmässä
https://converis.jyu.fi/converis/portal/detail/Publication/207645181
Metadata
Näytä kaikki kuvailutiedotKokoelmat
Rahoittaja(t)
Suomen AkatemiaRahoitusohjelmat(t)
Akatemiatutkija, SALisätietoja rahoituksesta
Financial support was provided by the European Commission (FET Open NEUROPA, grant 863214 to A.M.), the Deutsche Forschungsgemeinschaft (grant MO2192/4-2 to A.M), the Academy of Finland (grant 330678 to H.T.), a three-year grant from the University of Helsinki (to E.M and H.T.), the Finnish Cultural Foundation (grant 00220697 to E.M.), and a Bayreuth Humboldt Centre Senior Fellowship 2020 (to H.T ...
Lisenssi
Ellei muuten mainita, aineiston lisenssi on CC BY-NC-ND 4.0