Multimodal Control of Bacterial Gene Expression by Red and Blue Light
| dc.contributor.author | Meier, Stefanie S. M. | |
| dc.contributor.author | Multamäki, Elina | |
| dc.contributor.author | Ranzani, Américo T. | |
| dc.contributor.author | Takala, Heikki | |
| dc.contributor.author | Möglich, Andreas | |
| dc.contributor.editor | Braman, Jeffrey Carl | |
| dc.date.accessioned | 2024-04-26T10:46:21Z | |
| dc.date.available | 2024-04-26T10:46:21Z | |
| dc.date.issued | 2024 | |
| dc.identifier.citation | Meier, S. S. M., Multamäki, E., Ranzani, A. T., Takala, H., & Möglich, A. (2024). Multimodal Control of Bacterial Gene Expression by Red and Blue Light. In J. C. Braman (Ed.), <i>Synthetic Biology : Methods and Protocols</i> (pp. 463-477). Humana Press. Methods in Molecular Biology, 2760. <a href="https://doi.org/10.1007/978-1-0716-3658-9_26" target="_blank">https://doi.org/10.1007/978-1-0716-3658-9_26</a> | |
| dc.identifier.other | CONVID_207645181 | |
| dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/94517 | |
| dc.description.abstract | By applying sensory photoreceptors, optogenetics realizes the light-dependent control of cellular events and state. Given reversibility, noninvasiveness, and exquisite spatiotemporal precision, optogenetic approaches enable innovative use cases in cell biology, synthetic biology, and biotechnology. In this chapter, we detail the implementation of the pREDusk, pREDawn, pCrepusculo, and pAurora optogenetic circuits for controlling bacterial gene expression by red and blue light, respectively. The protocols provided here guide the practical use and multiplexing of these circuits, thereby enabling graded protein production in bacteria at analytical and semi-preparative scales. | en |
| dc.format.extent | 527 | |
| dc.format.mimetype | application/pdf | |
| dc.language.iso | eng | |
| dc.publisher | Humana Press | |
| dc.relation.ispartof | Synthetic Biology : Methods and Protocols | |
| dc.relation.ispartofseries | Methods in Molecular Biology | |
| dc.rights | CC BY-NC-ND 4.0 | |
| dc.subject.other | ANTAR | |
| dc.subject.other | bacteriophytochrome | |
| dc.subject.other | gene expression | |
| dc.subject.other | histidine kinase | |
| dc.subject.other | light-oxygen-voltage | |
| dc.subject.other | optogenetics | |
| dc.subject.other | RNA binding | |
| dc.subject.other | sensory photoreceptor | |
| dc.subject.other | synthetic biology | |
| dc.subject.other | two-component system | |
| dc.title | Multimodal Control of Bacterial Gene Expression by Red and Blue Light | |
| dc.type | bookPart | |
| dc.identifier.urn | URN:NBN:fi:jyu-202404263144 | |
| dc.contributor.laitos | Bio- ja ympäristötieteiden laitos | fi |
| dc.contributor.laitos | Department of Biological and Environmental Science | en |
| dc.type.uri | http://purl.org/eprint/type/BookItem | |
| dc.relation.isbn | 978-1-0716-3657-2 | |
| dc.type.coar | http://purl.org/coar/resource_type/c_3248 | |
| dc.description.reviewstatus | peerReviewed | |
| dc.format.pagerange | 463-477 | |
| dc.relation.issn | 1064-3745 | |
| dc.type.version | acceptedVersion | |
| dc.rights.copyright | © 2024 the Authors | |
| dc.rights.accesslevel | embargoedAccess | fi |
| dc.relation.grantnumber | 330678 | |
| dc.subject.yso | RNA | |
| dc.subject.yso | optogenetiikka | |
| dc.subject.yso | geeniekspressio | |
| dc.subject.yso | synteettinen biologia | |
| dc.format.content | fulltext | |
| jyx.subject.uri | http://www.yso.fi/onto/yso/p7689 | |
| jyx.subject.uri | http://www.yso.fi/onto/yso/p27165 | |
| jyx.subject.uri | http://www.yso.fi/onto/yso/p25831 | |
| jyx.subject.uri | http://www.yso.fi/onto/yso/p27811 | |
| dc.rights.url | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.relation.doi | 10.1007/978-1-0716-3658-9_26 | |
| dc.relation.funder | Research Council of Finland | en |
| dc.relation.funder | Suomen Akatemia | fi |
| jyx.fundingprogram | Academy Research Fellow, AoF | en |
| jyx.fundingprogram | Akatemiatutkija, SA | fi |
| jyx.fundinginformation | Financial support was provided by the European Commission (FET Open NEUROPA, grant 863214 to A.M.), the Deutsche Forschungsgemeinschaft (grant MO2192/4-2 to A.M), the Academy of Finland (grant 330678 to H.T.), a three-year grant from the University of Helsinki (to E.M and H.T.), the Finnish Cultural Foundation (grant 00220697 to E.M.), and a Bayreuth Humboldt Centre Senior Fellowship 2020 (to H.T.). | |
| dc.type.okm | A3 |
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