Multimodal Control of Bacterial Gene Expression by Red and Blue Light

Meier, Stefanie S. M.; Multamäki, Elina; Ranzani, Américo T.; Takala, Heikki; Möglich, Andreas

doi:10.1007/978-1-0716-3658-9_26

dc.contributor.author	Meier, Stefanie S. M.
dc.contributor.author	Multamäki, Elina
dc.contributor.author	Ranzani, Américo T.
dc.contributor.author	Takala, Heikki
dc.contributor.author	Möglich, Andreas
dc.contributor.editor	Braman, Jeffrey Carl
dc.date.accessioned	2024-04-26T10:46:21Z
dc.date.available	2024-04-26T10:46:21Z
dc.date.issued	2024
dc.identifier.citation	Meier, S. S. M., Multamäki, E., Ranzani, A. T., Takala, H., & Möglich, A. (2024). Multimodal Control of Bacterial Gene Expression by Red and Blue Light. In J. C. Braman (Ed.), <i>Synthetic Biology : Methods and Protocols</i> (pp. 463-477). Humana Press. Methods in Molecular Biology, 2760. <a href="https://doi.org/10.1007/978-1-0716-3658-9_26" target="_blank">https://doi.org/10.1007/978-1-0716-3658-9_26</a>
dc.identifier.other	CONVID_207645181
dc.identifier.uri	https://jyx.jyu.fi/handle/123456789/94517
dc.description.abstract	By applying sensory photoreceptors, optogenetics realizes the light-dependent control of cellular events and state. Given reversibility, noninvasiveness, and exquisite spatiotemporal precision, optogenetic approaches enable innovative use cases in cell biology, synthetic biology, and biotechnology. In this chapter, we detail the implementation of the pREDusk, pREDawn, pCrepusculo, and pAurora optogenetic circuits for controlling bacterial gene expression by red and blue light, respectively. The protocols provided here guide the practical use and multiplexing of these circuits, thereby enabling graded protein production in bacteria at analytical and semi-preparative scales.	en
dc.format.extent	527
dc.format.mimetype	application/pdf
dc.language.iso	eng
dc.publisher	Humana Press
dc.relation.ispartof	Synthetic Biology : Methods and Protocols
dc.relation.ispartofseries	Methods in Molecular Biology
dc.rights	CC BY-NC-ND 4.0
dc.subject.other	ANTAR
dc.subject.other	bacteriophytochrome
dc.subject.other	gene expression
dc.subject.other	histidine kinase
dc.subject.other	light-oxygen-voltage
dc.subject.other	optogenetics
dc.subject.other	RNA binding
dc.subject.other	sensory photoreceptor
dc.subject.other	synthetic biology
dc.subject.other	two-component system
dc.title	Multimodal Control of Bacterial Gene Expression by Red and Blue Light
dc.type	book part
dc.identifier.urn	URN:NBN:fi:jyu-202404263144
dc.contributor.laitos	Bio- ja ympäristötieteiden laitos	fi
dc.contributor.laitos	Department of Biological and Environmental Science	en
dc.type.uri	http://purl.org/eprint/type/BookItem
dc.relation.isbn	978-1-0716-3657-2
dc.type.coar	http://purl.org/coar/resource_type/c_3248
dc.description.reviewstatus	peerReviewed
dc.format.pagerange	463-477
dc.relation.issn	1064-3745
dc.type.version	acceptedVersion
dc.rights.copyright	© 2024 the Authors
dc.rights.accesslevel	embargoedAccess	fi
dc.type.publication	bookPart
dc.relation.grantnumber	330678
dc.subject.yso	RNA
dc.subject.yso	optogenetiikka
dc.subject.yso	geeniekspressio
dc.subject.yso	synteettinen biologia
dc.format.content	fulltext
jyx.subject.uri	http://www.yso.fi/onto/yso/p7689
jyx.subject.uri	http://www.yso.fi/onto/yso/p27165
jyx.subject.uri	http://www.yso.fi/onto/yso/p25831
jyx.subject.uri	http://www.yso.fi/onto/yso/p27811
dc.rights.url	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.relation.doi	10.1007/978-1-0716-3658-9_26
dc.relation.funder	Research Council of Finland	en
dc.relation.funder	Suomen Akatemia	fi
jyx.fundingprogram	Academy Research Fellow, AoF	en
jyx.fundingprogram	Akatemiatutkija, SA	fi
jyx.fundinginformation	Financial support was provided by the European Commission (FET Open NEUROPA, grant 863214 to A.M.), the Deutsche Forschungsgemeinschaft (grant MO2192/4-2 to A.M), the Academy of Finland (grant 330678 to H.T.), a three-year grant from the University of Helsinki (to E.M and H.T.), the Finnish Cultural Foundation (grant 00220697 to E.M.), and a Bayreuth Humboldt Centre Senior Fellowship 2020 (to H.T.).
dc.type.okm	A3

Aineistoon kuuluvat tiedostot

Nimi:: Meier_4OpenAccess.pdf
Koko:: 5.017Mb
Tiedostomuoto:: PDF
Kuvaus:: acceptedVersion

Katso/Avaa

Aineisto kuuluu seuraaviin kokoelmiin

Matemaattis-luonnontieteellinen tiedekunta [6159]

Näytä suppeat kuvailutiedot

Ellei muuten mainita, aineiston lisenssi on CC BY-NC-ND 4.0

Näytetään aineistoja, joilla on samankaltainen nimeke tai asiasanat.

Optogenetic Control of Bacterial Expression by Red Light

Multamäki, Elina; García de Fuentes, Andrés; Sieryi, Oleksii; Bykov, Alexander; Gerken, Uwe; Ranzani, Américo Tavares; Köhler, Jürgen; Meglinski, Igor; Möglich, Andreas; Takala, Heikki (American Chemical Society (ACS), 2022)

In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) photoreceptors sense red and far-red light via a biliverdin chromophore and, in response, cycle ...
Designing and testing red light-activated optogenetic tool for controlling gene expression in mammalian cells

Sutinen, Satu (2024)

Optogenetiikka tutkii ja ohjaa soluja valoherkkien proteiinien, valoreseptorien avulla. Eräs valoreseptorityyppi on bakteerifytokromit, jotka aistivat punaista ja kaukopunaista valoa. Luonnossa bakteerifytokromit ovat usein ...
The effects of photosensory module components for phytochrome activity and spectroscopy

Vanhatalo, Roosa (2023)

Bakteerifytokromit voivat säädellä isäntäsolun sisäistä metaboliaa valo-olosuhteista riippuen. Tämän mahdollistaa se, että fytokromit voivat toimia osana kaksikomponenttijärjestelmiä, jotka voivat puolestaan säädellä geenien ...
Biological control of potato soft rot caused by Dickeya solani and the survival of bacterial antagonists under cold storage conditions

Hadizadeh, I.; Peivastegan, B.; Hannukkala, A.; van der Wolf, J. M.; Nissinen, Riitta; Pirhonen, M. (Blackwell Scientific, 2019)

Dickeya and Pectobacterium are responsible for causing blackleg of plants and soft rot of tubers in storage and in the field, giving rise to losses in seed potato production. In an attempt to improve potato health, biocontrol ...
Cryo-EM structures of a bathy phytochrome histidine kinase reveal a unique light-dependent activation mechanism

Bódizs, Szabolcs; Mészáros, Petra; Grunewald, Lukas; Takala, Heikki; Westenhoff, Sebastian (Elsevier, 2024)

Phytochromes are photoreceptor proteins in plants, fungi, and bacteria. They can adopt two photochromic states with differential biochemical responses. The structural changes transducing the signal from the chromophore to ...

Multimodal Control of Bacterial Gene Expression by Red and Blue Light

Aineistoon kuuluvat tiedostot

Aineisto kuuluu seuraaviin kokoelmiin

Samankaltainen aineisto

Optogenetic Control of Bacterial Expression by Red Light ﻿

Designing and testing red light-activated optogenetic tool for controlling gene expression in mammalian cells ﻿

The effects of photosensory module components for phytochrome activity and spectroscopy ﻿

Biological control of potato soft rot caused by Dickeya solani and the survival of bacterial antagonists under cold storage conditions ﻿

Cryo-EM structures of a bathy phytochrome histidine kinase reveal a unique light-dependent activation mechanism ﻿

Optogenetic Control of Bacterial Expression by Red Light

Designing and testing red light-activated optogenetic tool for controlling gene expression in mammalian cells

The effects of photosensory module components for phytochrome activity and spectroscopy

Biological control of potato soft rot caused by Dickeya solani and the survival of bacterial antagonists under cold storage conditions

Cryo-EM structures of a bathy phytochrome histidine kinase reveal a unique light-dependent activation mechanism