Multimodal Control of Bacterial Gene Expression by Red and Blue Light
Meier, S. S. M., Multamäki, E., Ranzani, A. T., Takala, H., & Möglich, A. (2024). Multimodal Control of Bacterial Gene Expression by Red and Blue Light. In J. C. Braman (Ed.), Synthetic Biology : Methods and Protocols (pp. 463-477). Humana Press. Methods in Molecular Biology, 2760. https://doi.org/10.1007/978-1-0716-3658-9_26
Published in
Methods in Molecular BiologyAuthors
Editors
Date
2024Access restrictions
Embargoed until: 2025-03-13Request copy from author
Copyright
© 2024 the Authors
By applying sensory photoreceptors, optogenetics realizes the light-dependent control of cellular events and state. Given reversibility, noninvasiveness, and exquisite spatiotemporal precision, optogenetic approaches enable innovative use cases in cell biology, synthetic biology, and biotechnology. In this chapter, we detail the implementation of the pREDusk, pREDawn, pCrepusculo, and pAurora optogenetic circuits for controlling bacterial gene expression by red and blue light, respectively. The protocols provided here guide the practical use and multiplexing of these circuits, thereby enabling graded protein production in bacteria at analytical and semi-preparative scales.
Publisher
Humana PressParent publication ISBN
978-1-0716-3657-2Is part of publication
Synthetic Biology : Methods and ProtocolsISSN Search the Publication Forum
1064-3745Keywords
Publication in research information system
https://converis.jyu.fi/converis/portal/detail/Publication/207645181
Metadata
Show full item recordCollections
Related funder(s)
Research Council of FinlandFunding program(s)
Academy Research Fellow, AoFAdditional information about funding
Financial support was provided by the European Commission (FET Open NEUROPA, grant 863214 to A.M.), the Deutsche Forschungsgemeinschaft (grant MO2192/4-2 to A.M), the Academy of Finland (grant 330678 to H.T.), a three-year grant from the University of Helsinki (to E.M and H.T.), the Finnish Cultural Foundation (grant 00220697 to E.M.), and a Bayreuth Humboldt Centre Senior Fellowship 2020 (to H.T.). ...License
Related items
Showing items with similar title or keywords.
-
Optogenetic Control of Bacterial Expression by Red Light
Multamäki, Elina; García de Fuentes, Andrés; Sieryi, Oleksii; Bykov, Alexander; Gerken, Uwe; Ranzani, Américo Tavares; Köhler, Jürgen; Meglinski, Igor; Möglich, Andreas; Takala, Heikki (American Chemical Society (ACS), 2022)In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) photoreceptors sense red and far-red light via a biliverdin chromophore and, in response, cycle ... -
Comparative analysis of two paradigm bacteriophytochromes reveals opposite functionalities in two-component signaling
Multamäki, Elina; Nanekar, Rahul; Morozov, Dmitry; Lievonen, Topias; Golonka, David; Wahlgren, Weixiao Yuan; Stucki-Buchli, Brigitte; Rossi, Jari; Hytönen, Vesa P.; Westenhoff, Sebastian; Ihalainen, Janne A.; Möglich, Andreas; Takala, Heikki (Springer Science and Business Media LLC, 2021)Bacterial phytochrome photoreceptors usually belong to two-component signaling systems which transmit environmental stimuli to a response regulator through a histidine kinase domain. Phytochromes switch between red ... -
Leveraging the histidine kinase-phosphatase duality to sculpt two-component signaling
Meier, Stefanie S. M.; Multamäki, Elina; Ranzani, Américo T.; Takala, Heikki; Möglich, Andreas (Nature Publishing Group, 2024)Bacteria must constantly probe their environment for rapid adaptation, a crucial need most frequently served by two-component systems (TCS). As one component, sensor histidine kinases (SHK) control the phosphorylation of ... -
Biological control of potato soft rot caused by Dickeya solani and the survival of bacterial antagonists under cold storage conditions
Hadizadeh, I.; Peivastegan, B.; Hannukkala, A.; van der Wolf, J. M.; Nissinen, Riitta; Pirhonen, M. (Blackwell Scientific, 2019)Dickeya and Pectobacterium are responsible for causing blackleg of plants and soft rot of tubers in storage and in the field, giving rise to losses in seed potato production. In an attempt to improve potato health, biocontrol ... -
Cryo-EM structures of a bathy phytochrome histidine kinase reveal a unique light-dependent activation mechanism
Bódizs, Szabolcs; Mészáros, Petra; Grunewald, Lukas; Takala, Heikki; Westenhoff, Sebastian (Elsevier, 2024)Phytochromes are photoreceptor proteins in plants, fungi, and bacteria. They can adopt two photochromic states with differential biochemical responses. The structural changes transducing the signal from the chromophore to ...