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dc.contributor.authorJonsdottir, Ilmur
dc.contributor.authorGiven, Cindy
dc.contributor.authorPenttinen, Reetta
dc.contributor.authorJalasvuori, Matti
dc.date.accessioned2023-04-20T11:12:10Z
dc.date.available2023-04-20T11:12:10Z
dc.date.issued2023
dc.identifier.citationJonsdottir, I., Given, C., Penttinen, R., & Jalasvuori, M. (2023). Preceding Host History of Conjugative Resistance Plasmids Affects Intra- and Interspecific Transfer Potential from Biofilm. <i>mSphere</i>, <i>8</i>(3), Article e00107-23. <a href="https://doi.org/10.1128/msphere.00107-23" target="_blank">https://doi.org/10.1128/msphere.00107-23</a>
dc.identifier.otherCONVID_182774258
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/86476
dc.description.abstractConjugative plasmids can confer antimicrobial resistance (AMR) to their host bacterium. The plasmids disperse even between distantly related host species, rescuing the host from otherwise detrimental effects of antibiotics. Little is known about the role of these plasmids in the spread of AMR during antibiotic treatment. One unstudied question is whether the past evolutionary history of a plasmid in a particular species creates host specificity in its rescue potential or if interspecific coevolution can improve interspecific rescues. To study this, we coevolved the plasmid RP4 under three different host settings; solely Escherichia coli or Klebsiella pneumoniae, or alternating between both of them. The ability of evolved plasmids in bacterial biofilm to rescue susceptible planktonic host bacteria of either the same or different species during beta-lactam treatment was tested. The interspecific coevolution seemed to decrease rescue potential for the RP4 plasmid, while the K. pneumoniae evolved plasmid became more host specific. Large deletion in the region encoding the mating pair formation (Tra2) apparatus was detected in the plasmids evolved with K. pneumoniae. This adaptation resulted in the exapted evolution of resistance against a plasmid-dependent bacteriophage PRD1. Further, previous studies have suggested that mutations in this region completely abolish the plasmid’s ability to conjugate; however, our study shows it is not essential for conjugation but rather affects the host-specific conjugation efficiency. Overall, the results suggest that previous evolutionary history can result in the separation of host-specific plasmid lineages that may be further amplified by unselected exaptations such as phage resistance. IMPORTANCE Antimicrobial resistance (AMR) is a major global public health threat which can rapidly spread in microbial communities via conjugative plasmids. Here, we advance with evolutionary rescue via conjugation in a more natural setting, namely, biofilm, and incorporate a broad-host range plasmid RP4 to test whether intra- and interspecific host histories affect its transfer potential. Escherichia coli and Klebsiella pneumoniae hosts were seen to elicit different evolutionary influences on the RP4 plasmid, leading to clear differences in the rescue potential and underlining the significant role of the plasmid-host interactions in the spread of AMR. We also contradicted previous reports that established certain conjugal transfer genes of RP4 as essential. This work enhances the understanding of how plasmid host range evolve in different host settings and further, the potential effects it may have on the horizontal spread of AMR in complex environments such as biofilms.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofseriesmSphere
dc.rightsCC BY 4.0
dc.subject.otherantibiotic resistance
dc.subject.otherbiofilms
dc.subject.otherevolutionary rescue
dc.subject.otherexperimental evolution
dc.subject.otherhorizontal gene transfer (HGT)
dc.subject.otherhost-plasmid interactions
dc.subject.otherplasmids
dc.titlePreceding Host History of Conjugative Resistance Plasmids Affects Intra- and Interspecific Transfer Potential from Biofilm
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202304202591
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineEkologia ja evoluutiobiologiafi
dc.contributor.oppiaineBiologisten vuorovaikutusten huippututkimusyksikköfi
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centeren
dc.contributor.oppiaineEcology and Evolutionary Biologyen
dc.contributor.oppiaineCentre of Excellence in Biological Interactions Researchen
dc.contributor.oppiaineCell and Molecular Biologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn2379-5042
dc.relation.numberinseries3
dc.relation.volume8
dc.type.versionpublishedVersion
dc.rights.copyright© 2023 the Authors
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber347531
dc.relation.grantnumber336518
dc.subject.ysobakteerit
dc.subject.ysoantibioottiresistenssi
dc.subject.ysobiofilmit
dc.subject.ysoplasmidit
dc.subject.ysohorisontaalinen geeninsiirto
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p1749
jyx.subject.urihttp://www.yso.fi/onto/yso/p29640
jyx.subject.urihttp://www.yso.fi/onto/yso/p25266
jyx.subject.urihttp://www.yso.fi/onto/yso/p27720
jyx.subject.urihttp://www.yso.fi/onto/yso/p29551
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1128/msphere.00107-23
dc.relation.funderResearch Council of Finlanden
dc.relation.funderResearch Council of Finlanden
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
jyx.fundingprogramAcademy Project, AoFen
jyx.fundingprogramOthers, AoFen
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundingprogramMuut, SAfi
jyx.fundinginformationI.J. was supported by the University of Jyväskylä Graduate School for Doctoral Studies (Department of Biological and Environmental Science). R.P. was supported by Academy of Finland (grant number 322204). M.J. was supported by Academy of Finland (grants number 347531 and number 336518) and Jane and Aatos Erkko Foundation.
dc.type.okmA1


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