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dc.contributor.authorMattola, Salla
dc.contributor.authorSalokas, Kari
dc.contributor.authorAho, Vesa
dc.contributor.authorMäntylä, Elina
dc.contributor.authorSalminen, Sami
dc.contributor.authorHakanen, Satu
dc.contributor.authorNiskanen, Einari A.
dc.contributor.authorSvirskaite, Julija
dc.contributor.authorIhalainen, Teemu O.
dc.contributor.authorAirenne, Kari J.
dc.contributor.authorKaikkonen-Määttä, Minna
dc.contributor.authorParrish, Colin R.
dc.contributor.authorVarjosalo, Markku
dc.contributor.authorVihinen-Ranta, Maija
dc.date.accessioned2022-05-23T07:57:43Z
dc.date.available2022-05-23T07:57:43Z
dc.date.issued2022
dc.identifier.citationMattola, S., Salokas, K., Aho, V., Mäntylä, E., Salminen, S., Hakanen, S., Niskanen, E. A., Svirskaite, J., Ihalainen, T. O., Airenne, K. J., Kaikkonen-Määttä, M., Parrish, C. R., Varjosalo, M., & Vihinen-Ranta, M. (2022). Parvovirus nonstructural protein 2 interacts with chromatin-regulating cellular proteins. <i>PLoS Pathogens</i>, <i>18</i>(4), Article e1010353. <a href="https://doi.org/10.1371/journal.ppat.1010353" target="_blank">https://doi.org/10.1371/journal.ppat.1010353</a>
dc.identifier.otherCONVID_117797069
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/81225
dc.description.abstractAutonomous parvoviruses encode at least two nonstructural proteins, NS1 and NS2. While NS1 is linked to important nuclear processes required for viral replication, much less is known about the role of NS2. Specifically, the function of canine parvovirus (CPV) NS2 has remained undefined. Here we have used proximity-dependent biotin identification (BioID) to screen for nuclear proteins that associate with CPV NS2. Many of these associations were seen both in noninfected and infected cells, however, the major type of interacting proteins shifted from nuclear envelope proteins to chromatin-associated proteins in infected cells. BioID interactions revealed a potential role for NS2 in DNA remodeling and damage response. Studies of mutant viral genomes with truncated forms of the NS2 protein suggested a change in host chromatin accessibility. Moreover, further studies with NS2 mutants indicated that NS2 performs functions that affect the quantity and distribution of proteins linked to DNA damage response. Notably, mutation in the splice donor site of the NS2 led to a preferred formation of small viral replication center foci instead of the large coalescent centers seen in wild-type infection. Collectively, our results provide insights into potential roles of CPV NS2 in controlling chromatin remodeling and DNA damage response during parvoviral replication.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherPublic Library of Science (PLoS)
dc.relation.ispartofseriesPLoS Pathogens
dc.rightsCC BY 4.0
dc.subject.otherparvoviruses
dc.subject.othercellular proteins
dc.titleParvovirus nonstructural protein 2 interacts with chromatin-regulating cellular proteins
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202205232855
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centeren
dc.contributor.oppiaineCell and Molecular Biologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.description.reviewstatuspeerReviewed
dc.relation.issn1553-7366
dc.relation.numberinseries4
dc.relation.volume18
dc.type.versionpublishedVersion
dc.rights.copyright© 2022 Mattola et al.
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber330896
dc.subject.ysoproteiinit
dc.subject.ysoinfektiot
dc.subject.ysoparvovirukset
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p4332
jyx.subject.urihttp://www.yso.fi/onto/yso/p7316
jyx.subject.urihttp://www.yso.fi/onto/yso/p21764
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1371/journal.ppat.1010353
dc.relation.funderSuomen Akatemiafi
dc.relation.funderAcademy of Finlanden
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundingprogramAcademy Project, AoFen
jyx.fundinginformationThis work was financed by the Jane and Aatos Erkko Foundation (MVR); Academy of Finland under the award number 330896 (MVR); Biocenter Finland, viral gene transfer (MVR), and the Graduate School of the University of Jyväskylä (SM).


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