Branched-Chain Amino Acid Deprivation Decreases Lipid Oxidation and Lipogenesis in C2C12 Myotubes
Karvinen, S., Fachada, V., Sahinaho, U.-M., Pekkala, S., Lautaoja, J. H., Mäntyselkä, S., Permi, P., Hulmi, J. J., Silvennoinen, M., & Kainulainen, H. (2022). Branched-Chain Amino Acid Deprivation Decreases Lipid Oxidation and Lipogenesis in C2C12 Myotubes. Metabolites, 12(4), Article 328. https://doi.org/10.3390/metabo12040328
Julkaistu sarjassa
MetabolitesTekijät
Päivämäärä
2022Oppiaine
LiikuntafysiologiaNanoscience CenterLiikuntalääketiedeExercise PhysiologyNanoscience CenterSports and Exercise MedicineTekijänoikeudet
© 2022 the Authors
Impaired lipid metabolism is a common risk factor underlying several metabolic diseases such as metabolic syndrome and type 2 diabetes. Branched-chain amino acids (BCAAs) that include valine, leucine and isoleucine have been proven to share a role in lipid metabolism and hence in maintaining metabolic health. We have previously introduced a hypothesis suggesting that BCAA degradation mechanistically connects to lipid oxidation and storage in skeletal muscle. To test our hypothesis, the present study examined the effects of BCAA deprivation and supplementation on lipid oxidation, lipogenesis and lipid droplet characteristics in murine C2C12 myotubes. In addition, the role of myotube contractions on cell metabolism was studied by utilizing in vitro skeletal-muscle-specific exercise-like electrical pulse stimulation (EPS). Our results showed that the deprivation of BCAAs decreased both lipid oxidation and lipogenesis in C2C12 myotubes. BCAA deprivation further diminished the number of lipid droplets in the EPS-treated myotubes. EPS decreased lipid oxidation especially when combined with high BCAA supplementation. Similar to BCAA deprivation, high BCAA supplementation also decreased lipid oxidation. The present results highlight the role of an adequate level of BCAAs in healthy lipid metabolism.
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MDPI AGISSN Hae Julkaisufoorumista
2218-1989Asiasanat
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https://converis.jyu.fi/converis/portal/detail/Publication/117775582
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This study was funded by a grant from the Academy of Finland (grant number 298875 to H.K.).Lisenssi
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