Branched-chain amino acid supplementation and voluntary running have distinct effects on the serum metabolome of rats with high or low intrinsic aerobic capacity
Karvinen, S., Korhonen, T.-M. K., Kiviö, R., Lensu, S., Gajera, B., Britton, S. L., Koch, L. G., Nieminen, A. I., & Kainulainen, H. (2024). Branched-chain amino acid supplementation and voluntary running have distinct effects on the serum metabolome of rats with high or low intrinsic aerobic capacity. Frontiers in Nutrition, 11, Article 145038. https://doi.org/10.3389/fnut.2024.1450386
Published in
Frontiers in NutritionAuthors
Date
2024Copyright
© 2024 Karvinen, Korhonen, Kiviö, Lensu,
Gajera, Britton, Koch, Nieminen and
Kainulaine
Introduction: A growing body of literature associates branched-chain amino acid (BCAA) catabolism to increased fatty acid oxidation and better metabolic health. Hence, BCAA-rich diets may improve body composition and muscle protein synthesis. However, the role of individual characteristics such as a low aerobic fitness, a well-established risk factor for cardio-metabolic diseases, has not been studied.
Methods: This study examined 64 female rats from the high-capacity runner (HCR) and low-capacity runner (LCR) rat model. Rats from each line (HCR or LCR) were divided into four groups; differing from diet (CTRL or BCAA) and from the opportunity to voluntarily run on a running wheel (NONRUNNER or RUNNER). Groups were matched for body mass and maximal running capacity within each line. We measured maximal running capacity and metabolism before and after the intervention of diet and voluntary running activity. After the end of the experiment, serum samples were collected for metabolome analysis.
Results: We are the first to show that BCAA supplementation has a more pronounced impact on LCRs compared to HCRs. Specifically, in LCR rats, BCAA supplementation led to reduced daily voluntary running distance and an enrichment of serine metabolism in the serum metabolome. While voluntary running increased food intake and energy expenditure, its effects on the serum metabolome were minimal in HCRs.
Conclusion: The present research highlights the benefit achieved by combining BCAA supplementation with running activity, especially in the LCR line. Importantly, our results underscore the interconnected role of BCAAs and fatty acid metabolism in promoting overall metabolic health.
...
Publisher
Frontiers Media SAISSN Search the Publication Forum
2296-861XKeywords
Publication in research information system
https://converis.jyu.fi/converis/portal/detail/Publication/244035223
Metadata
Show full item recordCollections
Related funder(s)
Research Council of FinlandFunding program(s)
Academy Project, AoFAdditional information about funding
This study was funded by a grant from the Academy of Finland (Grant Number: 298875 to HK). The LCR-HCR rat model system was funded by National Institutes of Health Office of Research Infrastructure Programs Grant Number: P40OD-021331 (to LK and SB).License
Related items
Showing items with similar title or keywords.
-
Effect of Aerobic Exercise and Time‐Restricted Feeding on Metabolic Markers and Circadian Rhythm in Mice Fed with the High‐Fat Diet
Raza, Ghulam Shere; Kaya, Yağmur; Stenbäck, Ville; Sharma, Ravikant; Sodum, Nalini; Mutt Shivaprakash, Jagalur; Gagnon, Dominique D.; Tulppo, Mikko; Järvelin, Marjo‐Riitta; Herzig, Karl‐Heinz; Mäkelä, Kari A. (Wiley-VCH Verlag, 2024)Scope Diet and exercise are significant players in obesity and metabolic diseases. Time-restricted feeding (tRF) has been shown to improve metabolic responses by regulating circadian clocks but whether it acts synergically ... -
Higher glucose availability augments the metabolic responses of the C2C12 myotubes to exercise-like electrical pulse stimulation
Lautaoja, Juulia H.; O'Connell, Thomas; Mäntyselkä, Sakari; Peräkylä, Juuli; Kainulainen, Heikki; Pekkala, Satu; Permi, Perttu; Hulmi, Juha J. (American Physiological Society, 2021)The application of exercise-like electrical pulse simulation (EL-EPS) has become a widely used exercise mimetic in vitro. EL-EPS produces similar physiological responses as in vivo exercise, while less is known about the ... -
Branched-chain amino acids and mitochondrial bioenergetics in C2C12 myoblasts
Lehtonen, Elias (2019)Johdanto. Haaraketjuiset aminohapot (HKAH, leusiini, isoleusiini, valiini) ja niiden aineenvaihdunta on liitetty parempaan aineenvaihdunnalliseen terveyteen, pidempään elinkaareen, parantuneeseen glukoosi- ja rasva-ainee ... -
The effects of physical activity and diet change on intracellular lipids and metabolism in mice with diet-induced obesity
Rinnankoski-Tuikka, Rita (University of Jyväskylä, 2014) -
The metabolic responses to electrical pulse-induced contractions in C2C12 myotubes are greater in high glucose medium
Mäntyselkä, Sakari (2021)Osaa liikunnan terveyshyötyjen mekanismeista on vaikeaa tutkia ihmisillä tai koe-eläimillä. Viljeltyjen C2C12 myotuubien sähköpulssistimulaatiolla (EPS, engl. electrical pulse stimulation) voidaan mallintaa lihassupistuksia ...