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dc.contributor.authorKarvonen, Kati
dc.contributor.authorTammisto, Hanna
dc.contributor.authorNykky, Jonna
dc.contributor.authorGilbert, Leona
dc.date.accessioned2022-01-27T06:49:58Z
dc.date.available2022-01-27T06:49:58Z
dc.date.issued2022
dc.identifier.citationKarvonen, K., Tammisto, H., Nykky, J., & Gilbert, L. (2022). Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells. <i>Microorganisms</i>, <i>10</i>(2), Article 212. <a href="https://doi.org/10.3390/microorganisms10020212" target="_blank">https://doi.org/10.3390/microorganisms10020212</a>
dc.identifier.otherCONVID_104005829
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/79536
dc.description.abstractLike many bacterial species, Borrelia burgdorferi, the pleomorphic bacterium that causes Lyme borreliosis, produces outer membrane vesicles (OMVs). Borrelial OMVs (BbOMVs) have been identified as containing virulence factors, such as outer surface proteins (Osps) A, B, and C, as well as DNA. However, the pathogenicity of BbOMVs in disease development is still unclear. In this study, we characterized purified BbOMVs by analyzing their size and immunolabeling for known antigenic markers: OspA, OspC, p39, and peptidoglycan. In addition, BbOMVs were cocultured with human non-immune cells for cytotoxicity analysis. The results demonstrated that, on average, the vesicles were small, ranging between 11 and 108 nm in diameter. In addition, both OspA and OspC, as well as Lyme arthritis markers p39 and peptidoglycan, were detected from BbOMVs. Furthermore, BbOMVs were cocultured with non-immune cells, which did not result in cell death. Combined, these results suggested that BbOMVs could participate in the induction of infection by functioning as a decoy for the host immune system. Furthermore, BbOMVs might serve as a means for persistent antigens to remain in the host for prolonged periods of time.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofseriesMicroorganisms
dc.rightsCC BY 4.0
dc.subject.otherLyme borreliosis
dc.subject.otherbleb
dc.subject.otherextracellular vesicle
dc.subject.otherpersistent antigen
dc.titleBorrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202201271305
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn2076-2607
dc.relation.numberinseries2
dc.relation.volume10
dc.type.versionpublishedVersion
dc.rights.copyright© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
dc.rights.accesslevelopenAccessfi
dc.subject.ysoBorrelia-bakteerit
dc.subject.ysoproteiinit
dc.subject.ysoborrelioosi
dc.subject.ysoantigeenit
dc.subject.ysobakteerit
dc.subject.ysoborrelioosi
dc.subject.ysoimmuniteetti
dc.subject.ysoimmunologia
dc.subject.ysoimmuunivaste
dc.subject.ysoimmuunijärjestelmä
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p23656
jyx.subject.urihttp://www.yso.fi/onto/yso/p4332
jyx.subject.urihttp://www.yso.fi/onto/yso/p13975
jyx.subject.urihttp://www.yso.fi/onto/yso/p12205
jyx.subject.urihttp://www.yso.fi/onto/yso/p1749
jyx.subject.urihttp://www.yso.fi/onto/yso/p13976
jyx.subject.urihttp://www.yso.fi/onto/yso/p7524
jyx.subject.urihttp://www.yso.fi/onto/yso/p18810
jyx.subject.urihttp://www.yso.fi/onto/yso/p21599
jyx.subject.urihttp://www.yso.fi/onto/yso/p16041
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.3390/microorganisms10020212
jyx.fundinginformationThis research was funded by the Schwartz foundation.
dc.type.okmA1


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