Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells
Karvonen, K., Tammisto, H., Nykky, J., & Gilbert, L. (2022). Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells. Microorganisms, 10(2), Article 212. https://doi.org/10.3390/microorganisms10020212
Published in
MicroorganismsDate
2022Copyright
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Like many bacterial species, Borrelia burgdorferi, the pleomorphic bacterium that causes Lyme borreliosis, produces outer membrane vesicles (OMVs). Borrelial OMVs (BbOMVs) have been identified as containing virulence factors, such as outer surface proteins (Osps) A, B, and C, as well as DNA. However, the pathogenicity of BbOMVs in disease development is still unclear. In this study, we characterized purified BbOMVs by analyzing their size and immunolabeling for known antigenic markers: OspA, OspC, p39, and peptidoglycan. In addition, BbOMVs were cocultured with human non-immune cells for cytotoxicity analysis. The results demonstrated that, on average, the vesicles were small, ranging between 11 and 108 nm in diameter. In addition, both OspA and OspC, as well as Lyme arthritis markers p39 and peptidoglycan, were detected from BbOMVs. Furthermore, BbOMVs were cocultured with non-immune cells, which did not result in cell death. Combined, these results suggested that BbOMVs could participate in the induction of infection by functioning as a decoy for the host immune system. Furthermore, BbOMVs might serve as a means for persistent antigens to remain in the host for prolonged periods of time.
...
Publisher
MDPI AGISSN Search the Publication Forum
2076-2607Keywords
Publication in research information system
https://converis.jyu.fi/converis/portal/detail/Publication/104005829
Metadata
Show full item recordCollections
Additional information about funding
This research was funded by the Schwartz foundation.License
Related items
Showing items with similar title or keywords.
-
Characterization and immunological aspects of Borrelia burgdorferi pleomorphic round bodies
Meriläinen, Leena (University of Jyväskylä, 2015) -
Distinctive Evasion Mechanisms to Allow Persistence of Borrelia burgdorferi in Different Human Cell Lines
Karvonen, Kati; Nykky, Jonna; Marjomäki, Varpu; Gilbert, Leona (Frontiers Research Foundation, 2021)Lyme borreliosis is a multisystemic disease caused by the pleomorphic bacteria of the Borrelia burgdorferi sensu lato complex. The exact mechanisms for the infection to progress into a prolonged sequelae of the disease are ... -
Unraveling the pleomorphic forms of Borrelia burgdorferi
Herranen, Anni (2014)Borrelia burgdorferi bakteeri on puutiaisten välittämän borrelioosin taudinaiheuttaja. Tämä tavanomaisesti spirokeettana esiintyvä bakteeri kykenee vaihtamaan muotoaan ympäristöolosuhteiden muuttuessa epäsuotuisiksi. ... -
Effects of rodent abundance on ticks and Borrelia: results from an experimental and observational study in an island system
Kiran, Nosheen; Brila, Ilze; Mappes, Tapio; Sipari, Saana; Wang, Yingying; Welsh, Erin; Kallio, Eva R. (BioMed Central, 2024)Background Lyme borreliosis is the most common tick-borne disease in Europe and is often caused by Borrelia afzelii, which is transmitted by Ixodes ricinus ticks. The prevalence and abundance of infected ticks fluctuate ... -
Comparing the Climatic and Landscape Risk Factors for Lyme Disease Cases in the Upper Midwest and Northeast United States
Dong, Yuting; Huang, Zheng; Zhang, Yong; Wang, X.G. Yingying; La, Yang (MDPI, 2020)Lyme disease, recognized as one of the most important vector-borne diseases worldwide, has been increasing in incidence and spatial extend in United States. In the Northeast and Upper Midwest, Lyme disease is transmitted ...