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dc.contributor.authorTienaho, Jenni
dc.contributor.authorReshamwala, Dhanik
dc.contributor.authorSarjala, Tytti
dc.contributor.authorKilpeläinen, Petri
dc.contributor.authorLiimatainen, Jaana
dc.contributor.authorDou, Jinze
dc.contributor.authorViherä-Aarnio, Anneli
dc.contributor.authorLinnakoski, Riikka
dc.contributor.authorMarjomäki, Varpu
dc.contributor.authorJyske, Tuula
dc.date.accessioned2022-01-26T07:11:29Z
dc.date.available2022-01-26T07:11:29Z
dc.date.issued2021
dc.identifier.citationTienaho, J., Reshamwala, D., Sarjala, T., Kilpeläinen, P., Liimatainen, J., Dou, J., Viherä-Aarnio, A., Linnakoski, R., Marjomäki, V., & Jyske, T. (2021). Salix spp. Bark Hot Water Extracts Show Antiviral, Antibacterial, and Antioxidant Activities : The Bioactive Properties of 16 Clones. <i>Frontiers in Bioengineering and Biotechnology</i>, <i>9</i>, Article 797939. <a href="https://doi.org/10.3389/fbioe.2021.797939" target="_blank">https://doi.org/10.3389/fbioe.2021.797939</a>
dc.identifier.otherCONVID_103996865
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/79525
dc.description.abstractEarlier studies have shown that the bark of Salix L. species (Salicaceae family) is rich in extractives, such as diverse bioactive phenolic compounds. However, we lack knowledge on the bioactive properties of the bark of willow species and clones adapted to the harsh climate conditions of the cool temperate zone. Therefore, the present study aimed to obtain information on the functional profiles of northern willow clones for the use of value-added bioactive solutions. Of the 16 willow clones studied here, 12 were examples of widely distributed native Finnish willow species, including dark-leaved willow (S. myrsinifolia Salisb.) and tea-leaved willow (S. phylicifolia L.) (3 + 4 clones, respectively) and their natural and artificial hybrids (3 + 2 clones, respectively). The four remaining clones were commercial willow varieties from the Swedish willow breeding program. Hot water extraction of bark under mild conditions was carried out. Bioactivity assays were used to screen antiviral, antibacterial, antifungal, yeasticidal, and antioxidant activities, as well as the total phenolic content of the extracts. Additionally, we introduce a fast and less labor-intensive steam-debarking method for Salix spp. feedstocks. Clonal variation was observed in the antioxidant properties of the bark extracts of the 16 Salix spp. clones. High antiviral activity against a non-enveloped enterovirus, coxsackievirus A9, was found, with no marked differences in efficacy between the native clones. All the clones also showed antibacterial activity against Staphylococcus aureus and Escherichia coli, whereas no antifungal (Aspergillus brasiliensis) or yeasticidal (Candida albicans) efficacy was detected. When grouping the clone extract results into Salix myrsinifolia, Salix phylicifolia, native hybrid, artificial hybrid, and commercial clones, there was a significant difference in the activities between S. phylicifolia clone extracts and commercial clone extracts in the favor of S. phylicifolia in the antibacterial and antioxidant tests. In some antioxidant tests, S. phylicifolia clone extracts were also significantly more active than artificial clone extracts. Additionally, S. myrsinifolia clone extracts showed significantly higher activities in some antioxidant tests than commercial clone extracts and artificial clone extracts. Nevertheless, the bark extracts of native Finnish willow clones showed high bioactivity. The obtained knowledge paves the way towards developing high value-added biochemicals and other functional solutions based on willow biorefinery approaches.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherFrontiers Media SA
dc.relation.ispartofseriesFrontiers in Bioengineering and Biotechnology
dc.rightsCC BY 4.0
dc.subject.otherantimicrobial
dc.subject.otherantioxidant
dc.subject.otherantiviral
dc.subject.otherbark
dc.subject.otherdebarking
dc.subject.otherSalix spp.
dc.subject.otherwater-extracts
dc.titleSalix spp. Bark Hot Water Extracts Show Antiviral, Antibacterial, and Antioxidant Activities : The Bioactive Properties of 16 Clones
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202201261296
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn2296-4185
dc.relation.volume9
dc.type.versionpublishedVersion
dc.rights.copyright© 2021 the Authors
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber
dc.subject.ysoantioksidantit
dc.subject.ysoluonnonaineet
dc.subject.ysopuunkuori
dc.subject.ysokuorinta
dc.subject.ysofenoliset yhdisteet
dc.subject.ysobioaktiiviset yhdisteet
dc.subject.ysoantimikrobiset yhdisteet
dc.subject.ysouutto
dc.subject.ysopajut
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p4727
jyx.subject.urihttp://www.yso.fi/onto/yso/p6956
jyx.subject.urihttp://www.yso.fi/onto/yso/p24500
jyx.subject.urihttp://www.yso.fi/onto/yso/p9939
jyx.subject.urihttp://www.yso.fi/onto/yso/p23393
jyx.subject.urihttp://www.yso.fi/onto/yso/p28433
jyx.subject.urihttp://www.yso.fi/onto/yso/p21949
jyx.subject.urihttp://www.yso.fi/onto/yso/p1415
jyx.subject.urihttp://www.yso.fi/onto/yso/p5259
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.3389/fbioe.2021.797939
dc.relation.funderJane and Aatos Erkko Foundationen
dc.relation.funderJane ja Aatos Erkon säätiöfi
jyx.fundingprogramFoundationen
jyx.fundingprogramSäätiöfi
jyx.fundinginformationThis work was supported by Business Finland corona-co-creation funding for the project Antiviral Fibers—pilot with extracts from Finnish forests (grant: 40699/31/2020). This study was also funded by the Natural Resources Institute Finland’s strategic research funding to the projects “More, faster, higher quality: potential of short-rotation aspen and willow biomass for novel products in bioeconomy” (AspenWill) and “Added value potential of new and under-utilized fibre sources in Finnish value networks of green bioeconomy: prefeasibility, prototyping, and market acceptance” (VALUEPOT). In addition, Academy of Finland has supported this study via the project “Antivirals from Forest Biomasses: Structure, Function, and Applicability” (grant: 342250). Work was also funded by Jane and Aatos Erkko Foundation: Novel probes for discovering antivirals.
dc.type.okmA1


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