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dc.contributor.authorRissanen, Antti J.
dc.contributor.authorSaarela, Taija
dc.contributor.authorJäntti, Helena
dc.contributor.authorBuck, Moritz
dc.contributor.authorPeura, Sari
dc.contributor.authorAalto, Sanni L.
dc.contributor.authorOjala, Anne
dc.contributor.authorPumpanen, Jukka
dc.contributor.authorTiirola, Marja
dc.contributor.authorElvert, Marcus
dc.contributor.authorNykänen, Hannu
dc.date.accessioned2021-02-09T09:06:16Z
dc.date.available2021-02-09T09:06:16Z
dc.date.issued2021
dc.identifier.citationRissanen, A. J., Saarela, T., Jäntti, H., Buck, M., Peura, S., Aalto, S. L., Ojala, A., Pumpanen, J., Tiirola, M., Elvert, M., & Nykänen, H. (2021). Vertical stratification patterns of methanotrophs and their genetic controllers in water columns of oxygen-stratified boreal lakes. <i>Fems Microbiology Ecology</i>, <i>97</i>(2), Article fiaa252. <a href="https://doi.org/10.1093/femsec/fiaa252" target="_blank">https://doi.org/10.1093/femsec/fiaa252</a>
dc.identifier.otherCONVID_47467454
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/74055
dc.description.abstractThe vertical structuring of methanotrophic communities and its genetic controllers remain understudied in the water columns of oxygen-stratified lakes. Therefore, we used 16S rRNA gene sequencing to study the vertical stratification patterns of methanotrophs in two boreal lakes, Lake Kuivajärvi and Lake Lovojärvi. Furthermore, metagenomic analyses were done to assess the genomic characteristics of methanotrophs in Lovojärvi and a previously studied Lake Alinen Mustajärvi. The methanotroph communities were vertically structured along the oxygen gradient. Alphaproteobacterial methanotrophs preferred oxic water layers, while Methylococcales methanotrophs, consisting of putative novel genera and species, thrived especially at and below the oxic-anoxic interface and showed distinct depth variation patterns, which were not completely predictable by their taxonomic classification. Instead, genomic differences among Methylococcales methanotrophs explained their variable vertical depth patterns. Genes in COG categories L (Replication, recombination and repair) and S (Function unknown) were relatively high in metagenome-assembled-genomes representing Methylococcales thriving clearly below the oxic-anoxic interface, suggesting genetic adaptations for increased stress tolerance enabling living in the hypoxic/anoxic conditions. In contrast, genes in COG category N (Cell motility) were relatively high in metagenome-assembled-genomes of Methylococcales thriving at the oxic-anoxic interface, which suggests genetic adaptations for increased motility at the vertically fluctuating oxic-anoxic interface.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.language.isoeng
dc.publisherOxford University Press
dc.relation.ispartofseriesFems Microbiology Ecology
dc.rightsCC BY-NC 4.0
dc.subject.othermethanotroph
dc.subject.otherMethylococcales
dc.subject.othervertical structuring
dc.subject.other16S rRNA
dc.subject.othermetagenomics
dc.subject.othergenetic potential
dc.titleVertical stratification patterns of methanotrophs and their genetic controllers in water columns of oxygen-stratified boreal lakes
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202102091496
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineYmpäristötiedefi
dc.contributor.oppiaineAkvaattiset tieteetfi
dc.contributor.oppiaineEnvironmental Scienceen
dc.contributor.oppiaineAquatic Sciencesen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn0168-6496
dc.relation.numberinseries2
dc.relation.volume97
dc.type.versionpublishedVersion
dc.rights.copyright© 2020 the Authors
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber615146
dc.relation.grantnumber615146
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/615146/EU//
dc.subject.ysojärvet
dc.subject.ysoRNA
dc.subject.ysometaani
dc.subject.ysohappikato
dc.subject.ysokerrostuneisuus
dc.subject.ysomikrobisto
dc.subject.ysogenomiikka
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p9374
jyx.subject.urihttp://www.yso.fi/onto/yso/p7689
jyx.subject.urihttp://www.yso.fi/onto/yso/p13222
jyx.subject.urihttp://www.yso.fi/onto/yso/p11510
jyx.subject.urihttp://www.yso.fi/onto/yso/p12358
jyx.subject.urihttp://www.yso.fi/onto/yso/p27039
jyx.subject.urihttp://www.yso.fi/onto/yso/p5146
dc.rights.urlhttps://creativecommons.org/licenses/by-nc/4.0/
dc.relation.doi10.1093/femsec/fiaa252
dc.relation.funderEuropean Commissionen
dc.relation.funderEuroopan komissiofi
jyx.fundingprogramFP7 (EU's 7th Framework Programme)en
jyx.fundingprogramEU:n 7. puiteohjelma (FP7)fi
jyx.fundinginformationThis study was supported by Kone Foundation (Grant No. 201803224) for AJR, Olvi-säätiö (Grant No. 201720037), Maa-ja Vesitekniikan tuki ry (Grant No. 34348), the University of Eastern Finland Doctoral Programme in Environmental Physics, Health and Biology (EPHB) and Water JPI ERA-NET Cofund WaterWorks2017 and Academy of Finland (project No. 326818) for TS, the Deutsche Forschungsgemeinschaft through the cluster of Excellence EXC 309 “The Ocean in the Earth system” (project No. 49926684) for ME, Academy of Finland (project No. 286642 for AJR, project No. 275127 for HJ, project No. 310302 for SLA, and project No. 136455 and 140964 for HN), and European Research Council (ERC) CoG project No. 615146 for MT. The authors also acknowledge the Academy of Finland Centre of Excellence (project No. 272041, 118780 and 307331) and ARCTICFIRE-project (project No. 286685) funded by Academy of Finland for JP. In addition, the authors acknowledge University of Eastern Finland Water Research Programme funded by Olvi-säätiö, Jenny and Antti Wihuri Foundation and Saastamoinen Foundation for HJ.
dc.type.okmA1


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