Vertical stratification patterns of methanotrophs and their genetic controllers in water columns of oxygen-stratified boreal lakes
Rissanen, A. J., Saarela, T., Jäntti, H., Buck, M., Peura, S., Aalto, S. L., Ojala, A., Pumpanen, J., Tiirola, M., Elvert, M., & Nykänen, H. (2021). Vertical stratification patterns of methanotrophs and their genetic controllers in water columns of oxygen-stratified boreal lakes. Fems Microbiology Ecology, 97(2), Article fiaa252. https://doi.org/10.1093/femsec/fiaa252
Published inFems Microbiology Ecology
© 2020 the Authors
The vertical structuring of methanotrophic communities and its genetic controllers remain understudied in the water columns of oxygen-stratified lakes. Therefore, we used 16S rRNA gene sequencing to study the vertical stratification patterns of methanotrophs in two boreal lakes, Lake Kuivajärvi and Lake Lovojärvi. Furthermore, metagenomic analyses were done to assess the genomic characteristics of methanotrophs in Lovojärvi and a previously studied Lake Alinen Mustajärvi. The methanotroph communities were vertically structured along the oxygen gradient. Alphaproteobacterial methanotrophs preferred oxic water layers, while Methylococcales methanotrophs, consisting of putative novel genera and species, thrived especially at and below the oxic-anoxic interface and showed distinct depth variation patterns, which were not completely predictable by their taxonomic classification. Instead, genomic differences among Methylococcales methanotrophs explained their variable vertical depth patterns. Genes in COG categories L (Replication, recombination and repair) and S (Function unknown) were relatively high in metagenome-assembled-genomes representing Methylococcales thriving clearly below the oxic-anoxic interface, suggesting genetic adaptations for increased stress tolerance enabling living in the hypoxic/anoxic conditions. In contrast, genes in COG category N (Cell motility) were relatively high in metagenome-assembled-genomes of Methylococcales thriving at the oxic-anoxic interface, which suggests genetic adaptations for increased motility at the vertically fluctuating oxic-anoxic interface. ...
PublisherOxford University Press
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Related funder(s)European Commission
Funding program(s)FP7 (EU's 7th Framework Programme)
The content of the publication reflects only the author’s view. The funder is not responsible for any use that may be made of the information it contains.
Additional information about fundingThis study was supported by Kone Foundation (Grant No. 201803224) for AJR, Olvi-säätiö (Grant No. 201720037), Maa-ja Vesitekniikan tuki ry (Grant No. 34348), the University of Eastern Finland Doctoral Programme in Environmental Physics, Health and Biology (EPHB) and Water JPI ERA-NET Cofund WaterWorks2017 and Academy of Finland (project No. 326818) for TS, the Deutsche Forschungsgemeinschaft through the cluster of Excellence EXC 309 “The Ocean in the Earth system” (project No. 49926684) for ME, Academy of Finland (project No. 286642 for AJR, project No. 275127 for HJ, project No. 310302 for SLA, and project No. 136455 and 140964 for HN), and European Research Council (ERC) CoG project No. 615146 for MT. The authors also acknowledge the Academy of Finland Centre of Excellence (project No. 272041, 118780 and 307331) and ARCTICFIRE-project (project No. 286685) funded by Academy of Finland for JP. In addition, the authors acknowledge University of Eastern Finland Water Research Programme funded by Olvi-säätiö, Jenny and Antti Wihuri Foundation and Saastamoinen Foundation for HJ. ...
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Saarenheimo, Jatta (University of Jyväskylä, 2015)
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