Gammaproteobacterial methanotrophs dominate methanotrophy in aerobic and anaerobic layers of boreal lake waters
Rissanen, A., Saarenheimo, J., Tiirola, M., Peura, S., Aalto, S. L., Karvinen, A., & Nykänen, H. (2018). Gammaproteobacterial methanotrophs dominate methanotrophy in aerobic and anaerobic layers of boreal lake waters. Aquatic Microbial Ecology, 81(3), 257-276. https://doi.org/10.3354/ame01874
Julkaistu sarjassa
Aquatic Microbial EcologyTekijät
Päivämäärä
2018Tekijänoikeudet
© The authors 2018.
Small oxygen-stratified humic lakes of the boreal zone are important sources of
methane to the atmosphere. Although stable isotope profiling has indicated that a substantial part
of methane is already oxidized in the anaerobic water layers in these lakes, the contributions of
aerobic and anaerobic methanotrophs in the process are unknown. We used next-generation
sequencing of mcrA and 16S rRNA genes to characterize the microbial communities in the water
columns of 2 boreal lakes in Finland, Lake Alinen-Mustajärvi and Lake Mekkojärvi, and complemented
this with a shotgun metagenomic analysis from Alinen-Mustajärvi and an analysis of
pmoA genes and 16S rRNA, mcrA, and pmoA transcripts from Mekkojärvi. Furthermore, we
tested the effect of various electron acceptors and light on methane oxidation (13C-CH4 labeling)
in incubations of water samples collected from the lakes. Aerobic gammaproteobacterial methanotrophs
(order Methylococcales) exclusively dominated the methanotrophic community both
above and below the oxycline in the lakes. A novel lineage within Methylococcales, Candidatus
Methyloumidiphilus alinensis, defined here for the first time, dominated in Alinen-Mustajärvi,
while methanotrophs belonging to Methylobacter were more abundant in Mekkojärvi. Light
enhanced methane oxidation in the anoxic water layer, while alternative electron acceptors
(SO4
2−, Fe3+, Mn4+, and anthraquinone-2,6-disulfonate), except for NO3
−, suppressed the process.
Our results suggest that oxygenic photosynthesis potentially fuels methanotrophy below the aerobic
water layers in methane-rich boreal lakes. Furthermore, incubation results, together with the
detection of denitrification genes from metagenome-assembled genomes of gamma proteo -
bacterial methanotrophs, imply that boreal lake methanotrophs may couple methane oxidation
with NOx
− reduction in hypoxic conditions.
...
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0948-3055Asiasanat
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https://converis.jyu.fi/converis/portal/detail/Publication/28130902
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Vertical stratification patterns of methanotrophs and their genetic controllers in water columns of oxygen-stratified boreal lakes
Rissanen, Antti J.; Saarela, Taija; Jäntti, Helena; Buck, Moritz; Peura, Sari; Aalto, Sanni L.; Ojala, Anne; Pumpanen, Jukka; Tiirola, Marja; Elvert, Marcus; Nykänen, Hannu (Oxford University Press, 2021)The vertical structuring of methanotrophic communities and its genetic controllers remain understudied in the water columns of oxygen-stratified lakes. Therefore, we used 16S rRNA gene sequencing to study the vertical ... -
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Integrating Decomposers, Methane-Cycling Microbes and Ecosystem Carbon Fluxes Along a Peatland Successional Gradient in a Land Uplift Region
Juottonen, Heli; Kieman, Mirkka; Fritze, Hannu; Hamberg, Leena; Laine, Anna M.; Merilä, Päivi; Peltoniemi, Krista; Putkinen, Anuliina; Tuittila, Eeva-Stiina (Springer Science+Business Media, 2022)Peatlands are carbon dioxide (CO2) sinks that, in parallel, release methane (CH4). The peatland carbon (C) balance depends on the interplay of decomposer and CH4-cycling microbes, vegetation, and environmental conditions. ... -
Comprehensive dataset of shotgun metagenomes from oxygen stratified freshwater lakes and ponds
Buck, Moritz; Garcia, Sarahi L.; Fernandez, Leyden; Martin, Gaëtan; Martinez-Rodriguez, Gustavo A.; Saarenheimo, Jatta; Zopfi, Jakob; Bertilsson, Stefan; Peura, Sari (Nature Publishing Group, 2021)Stratified lakes and ponds featuring steep oxygen gradients are significant net sources of greenhouse gases and hotspots in the carbon cycle. Despite their significant biogeochemical roles, the microbial communities, ...
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