Näytä suppeat kuvailutiedot

dc.contributor.authorXu, Man
dc.contributor.authorPerdomo, Maria Fernanda
dc.contributor.authorMattola, Salla
dc.contributor.authorPyöriä, Lari
dc.contributor.authorToppinen, Mari
dc.contributor.authorQiu, Jianming
dc.contributor.authorVihinen-Ranta, Maija
dc.contributor.authorHedman, Klaus
dc.contributor.authorNokso-Koivisto, Johanna
dc.contributor.authorAaltonen, Leena-Maija
dc.contributor.authorSöderlund-Venermo, Maria
dc.date.accessioned2021-02-08T11:23:06Z
dc.date.available2021-02-08T11:23:06Z
dc.date.issued2021
dc.identifier.citationXu, M., Perdomo, M. F., Mattola, S., Pyöriä, L., Toppinen, M., Qiu, J., Vihinen-Ranta, M., Hedman, K., Nokso-Koivisto, J., Aaltonen, L.-M., & Söderlund-Venermo, M. (2021). Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection. <i>mBio</i>, <i>12</i>(1), Article e03132-20. <a href="https://doi.org/10.1128/mbio.03132-20" target="_blank">https://doi.org/10.1128/mbio.03132-20</a>
dc.identifier.otherCONVID_51369230
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/74021
dc.description.abstractHuman bocavirus 1 (HBoV1), a nonenveloped single-stranded DNA parvovirus, causes mild to life-threatening respiratory tract infections, acute otitis media, and encephalitis in young children. HBoV1 often persists in nasopharyngeal secretions for months, hampering diagnosis. It has also been shown to persist in pediatric palatine and adenoid tonsils, which suggests that lymphoid organs are reservoirs for virus spread; however, the tissue site and host cells remain unknown. Our aim was to determine, in healthy nonviremic children with preexisting HBoV1 immunity, the adenotonsillar persistence site(s), host cell types, and virus activity. We discovered that HBoV1 DNA persists in lymphoid germinal centers (GCs), but not in the corresponding tonsillar epithelium, and that the cell types harboring the virus are mainly naive, activated, and memory B cells and monocytes. Both viral DNA strands and both sides of the genome were detected, as well as infrequent mRNA. Moreover, we showed, in B-cell and monocyte cultures and ex vivo tonsillar B cells, that the cellular uptake of HBoV1 occurs via the Fc receptor (FcγRII) through antibody-dependent enhancement (ADE). This resulted in viral mRNA transcription, known to occur exclusively from double-stranded DNA in the nucleus, however, with no detectable productive replication. Confocal imaging with fluorescent virus-like particles moreover disclosed endocytosis. To which extent the active HBoV1 GC persistence has a role in chronic inflammation or B-cell maturation disturbances, and whether the virus can be reactivated, will be interesting topics for forthcoming studies.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofseriesmBio
dc.rightsCC BY 4.0
dc.subject.otherparvovirus
dc.subject.othergerminal center
dc.subject.othertonsils
dc.subject.othervirus persistence
dc.subject.otherADE
dc.subject.otherin situ hybridization
dc.titlePersistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202102081462
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn2161-2129
dc.relation.numberinseries1
dc.relation.volume12
dc.type.versionpublishedVersion
dc.rights.copyright© 2021 Xu et al.
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber330896
dc.relation.grantnumber101017116
dc.relation.grantnumber101017116
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/H2020/101017116/EU//CoCID
dc.subject.ysovirukset
dc.subject.ysoparvovirukset
dc.subject.ysonielurisat
dc.subject.ysoinfektiot
dc.subject.ysovasta-aineet
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p1123
jyx.subject.urihttp://www.yso.fi/onto/yso/p21764
jyx.subject.urihttp://www.yso.fi/onto/yso/p6043
jyx.subject.urihttp://www.yso.fi/onto/yso/p7316
jyx.subject.urihttp://www.yso.fi/onto/yso/p12206
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1128/mbio.03132-20
dc.relation.funderResearch Council of Finlanden
dc.relation.funderEuropean Commissionen
dc.relation.funderSuomen Akatemiafi
dc.relation.funderEuroopan komissiofi
jyx.fundingprogramAcademy Project, AoFen
jyx.fundingprogramRIA Research and Innovation Action, H2020en
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundingprogramRIA Research and Innovation Action, H2020fi
jyx.fundinginformationThis study was supported by the China Scholarship Council (M.X., 201406170014), the Finnish-Norwegian Medical Foundation (M.X., 201900123), the Sigrid Jusélius Foundation (M.S.-V.), the Life and Health Medical Support Association (M.S.-V.), the Jane and Aatos Erkko Foundation (K.H.; M.V.-R.), Academy of Finland (M.V.-R., 330896), European Union’s Horizon 2020 research and innovation program (M.V.-R., 101017116), project CoCID (Compact Cell-Imaging Device) (M.V.-R.), the Finnish Cultural Fund (M.T.), the Helsinki University Hospital Research Funds (L.-M.A., TYH2019100), and the Graduate School of the University of Jyväskylä (S.M., 2018 to 2021).
dc.type.okmA1


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