Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection
Xu, M., Perdomo, M. F., Mattola, S., Pyöriä, L., Toppinen, M., Qiu, J., Vihinen-Ranta, M., Hedman, K., Nokso-Koivisto, J., Aaltonen, L.-M., & Söderlund-Venermo, M. (2021). Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection. mBio, 12(1), Article e03132-20. https://doi.org/10.1128/mbio.03132-20
Xu, Man |
© 2021 Xu et al.
Human bocavirus 1 (HBoV1), a nonenveloped single-stranded DNA parvovirus, causes mild to life-threatening respiratory tract infections, acute otitis media, and encephalitis in young children. HBoV1 often persists in nasopharyngeal secretions for months, hampering diagnosis. It has also been shown to persist in pediatric palatine and adenoid tonsils, which suggests that lymphoid organs are reservoirs for virus spread; however, the tissue site and host cells remain unknown. Our aim was to determine, in healthy nonviremic children with preexisting HBoV1 immunity, the adenotonsillar persistence site(s), host cell types, and virus activity. We discovered that HBoV1 DNA persists in lymphoid germinal centers (GCs), but not in the corresponding tonsillar epithelium, and that the cell types harboring the virus are mainly naive, activated, and memory B cells and monocytes. Both viral DNA strands and both sides of the genome were detected, as well as infrequent mRNA. Moreover, we showed, in B-cell and monocyte cultures and ex vivo tonsillar B cells, that the cellular uptake of HBoV1 occurs via the Fc receptor (FcγRII) through antibody-dependent enhancement (ADE). This resulted in viral mRNA transcription, known to occur exclusively from double-stranded DNA in the nucleus, however, with no detectable productive replication. Confocal imaging with fluorescent virus-like particles moreover disclosed endocytosis. To which extent the active HBoV1 GC persistence has a role in chronic inflammation or B-cell maturation disturbances, and whether the virus can be reactivated, will be interesting topics for forthcoming studies. ...
PublisherAmerican Society for Microbiology
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Related funder(s)Academy of Finland; European Commission
Funding program(s)Academy Project, AoF
The content of the publication reflects only the author’s view. The funder is not responsible for any use that may be made of the information it contains.
Additional information about fundingThis study was supported by the China Scholarship Council (M.X., 201406170014), the Finnish-Norwegian Medical Foundation (M.X., 201900123), the Sigrid Jusélius Foundation (M.S.-V.), the Life and Health Medical Support Association (M.S.-V.), the Jane and Aatos Erkko Foundation (K.H.; M.V.-R.), Academy of Finland (M.V.-R., 330896), European Union’s Horizon 2020 research and innovation program (M.V.-R., 101017116), project CoCID (Compact Cell-Imaging Device) (M.V.-R.), the Finnish Cultural Fund (M.T.), the Helsinki University Hospital Research Funds (L.-M.A., TYH2019100), and the Graduate School of the University of Jyväskylä (S.M., 2018 to 2021). ...
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