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Infection under the ion beam : focused ion beams and antibacterial properties of biomaterials

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JYU dissertations
Authors
Leppänen, Miika
Date
2020

 
In this thesis, Helium Ion Microscopy (HIM) imaging and milling on organic and antibacterial materials will be discussed. In addition, I will discuss the antibacterial properties of surface-immobilized bacteriophages. HIM is a recently developed imaging method, which is especially suitable for biological samples because they can be imaged without a metal coating. Because of the state-of-the-art ion source, the microscope has also a higher imaging resolution compared to the scanning electron microscope (SEM). The suitability of HIM imaging for the bacteria-phage interactions is discussed in addition to the more delicate nanocellulose samples. High beam damage on the cellulose was found which has not been reported previously with HIM. Indeed an ion beam can have a milling property, which is demonstrated here with bacteria, bacteria-dragonfly interactions and encapsulated bacteriophages to obtain volumetric information. The antibacterial effectivity of immobilized bacteriophages was analyzed quantitatively with HIM-imaging and biological measures. It was found that detaching phages from the material are likely the major contributor to the infectivity of the phage-biomaterial. ...
ISBN
ISBN 978-951-39-8187-7
Contains publications
  • Artikkeli I: Leppänen, Miika; Maasilta, Ilari; Sundberg, Lotta-Riina (2019). Antibacterial efficiency of surface-immobilized Flavobacterium-infecting bacteriophage. ACS Applied Bio Materials, 2 (11), 4720-4727. DOI: 10.1021/acsabm.9b00242
  • Artikkeli II: Leppänen, M., Sundberg, L.-R., Laanto, E., De Freitas Almeida, G., Papponen, P., & Maasilta, I. (2017). Imaging Bacterial Colonies and Phage-Bacterium Interaction at Sub-Nanometer Resolution Using Helium-Ion Microscopy. Advanced Biosystems, 1 (8), 1700070. DOI: 10.1002/adbi.201700070
  • Artikkeli III: Ketola, A. E., Leppänen, M., Turpeinen, T., Papponen, P., Strand, A., Sundberg, A., . . . , & Retulainen, E. (2019). Cellulose nanofibrils prepared by gentle drying methods reveal the limits of helium ion microscopy imaging. RSC Advances, 9 (27), 15668-15677. DOI: 10.1039/C9RA01447K
  • Artikkeli IV: Vinner, G. K., Rezaie-Yazdi, Z., Leppänen, M., Stapley, A. G. F., Leaper, M. C., & Malik, D. J. (2019). Microencapsulation of Salmonella-Specific Bacteriophage Felix O1 Using Spray-Drying in a pH-Responsive Formulation and Direct Compression Tableting of Powders into a Solid Oral Dosage Form. Pharmaceuticals, 12 (1), 43. DOI: 10.3390/ph12010043
  • Artikkeli V: Vinner, Gurinder K.; Richards, Kerry; Leppänen, Miika; Sagona, Antonia P.; Malik 1, Danish J. (2019). Microencapsulation of Enteric Bacteriophages in a pH-Responsive Solid Oral Dosage Formulation Using a Scalable Membrane Emulsification Process. Pharmaceutics, 11 (9), 475. DOI: 10.3390/pharmaceutics11090475
  • Artikkeli VI: Bandara, Chaturanga D.; Ballerin, Giulia; Leppänen, Miika; Tesfamichael, Tuquabo; Ostrikov, Kostya (Ken); Whitchurch, Cynthia B. (2020). Resolving Bio-Nano Interactions of E.coli Bacteria-Dragonfly Wing Interface with Helium Ion and 3D-Structured Illumination Microscopy to Understand Bacterial Death on Nanotopography. ACS Biomaterials Science & Engineering, 6 (7), 3925-3932. DOI: 10.1021/acsbiomaterials.9b01973
  • Artikkeli VII: De Freitas Almeida, G., Leppänen, M., Maasilta, I., & Sundberg, L.-R. (2018). Bacteriophage imaging : past, present and future. Research in Microbiology, 169 (9), 488-494. DOI: 10.1016/j.resmic.2018.05.006
Keywords
mikroskopia fokusoidut ionisuihkut biomateriaalit bakteriofagit fagiterapia nanoselluloosa materiaalitutkimus heliumionimikroskopia helium ion microscopy focused ion beam milling nanocellulose phage therapy phage-biomaterial bacteriophage immobilization
URI

http://urn.fi/URN:ISBN:978-951-39-8187-7

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