The Fate of Bacteriophages in Recirculating Aquaculture Systems (RAS) : Towards Developing Phage Therapy for RAS
Almeida, G. M. F., Mäkelä, K., Laanto, E., Pulkkinen, J., Vielma, J., & Sundberg, L.-R. (2019). The Fate of Bacteriophages in Recirculating Aquaculture Systems (RAS) : Towards Developing Phage Therapy for RAS. Antibiotics, 8(4), Article 192. https://doi.org/10.3390/antibiotics8040192
Published in
AntibioticsAuthors
Date
2019Discipline
Solu- ja molekyylibiologiaBiologisten vuorovaikutusten huippututkimusyksikköCell and Molecular BiologyCentre of Excellence in Biological Interactions ResearchCopyright
© 2019 by the authors. Licensee MDPI, Basel, Switzerland
Aquaculture production has increased tremendously during the last decades, and new techniques have been developed, e.g., recirculating aquaculture systems (RAS). In RAS, the majority of water volume is circulated via mechanical and biological filters and reused in the tanks. However, the prevention and treatment of diseases in these systems are challenging, as the pathogens spread throughout the system, and the addition of chemicals and antibiotics disrupts the microbiome of the biofilters. The increasing antibiotic resistance has made phage therapy a relevant alternative for antibiotics in food production. Indeed, as host-specific and self-replicating agent they might be optimal for targeted pathogen eradication in RAS. We tested the survival and spread of Flavobacterium columnare -infecting phage FCL-2 in recirculating aquaculture fish farm with rainbow trout (Oncorhynchus mykiss) in a fully controlled study. After a single addition, phage persisted in water samples collected from tank, fixed bed, moving bed, and aeration unit up to 14 days, and in the water of rearing tanks, rainbow trout mucus, and bioreactor carrier media from the fixed and moving bed biofilters for 21 days. Furthermore, phage adsorbed preferentially to moving bed carrier media, which contained biofilm attached and from which higher phage numbers were recovered. This study shows phages as a potent strategy for maintaining biosecurity in RAS systems.
...
Publisher
MDPI AGISSN Search the Publication Forum
2079-6382Keywords
Publication in research information system
https://converis.jyu.fi/converis/portal/detail/Publication/33361583
Metadata
Show full item recordCollections
Related funder(s)
Research Council of FinlandFunding program(s)
Academy Project, AoFAdditional information about funding
We acknowledge funding from the fisheries innovation program of the European Maritime and Fisheries Fund (EMFF), Academy of Finland (grants #314939 and #321985), and Jane and Aatos Erkko Foundation. This work resulted from the BONUS FLAVOPHAGE project supported by BONUS (Art 185), funded jointly by the EU and Academy of Finland.License
Related items
Showing items with similar title or keywords.
-
Comparison of Delivery Methods in Phage Therapy against Flavobacterium columnare Infections in Rainbow Trout
Kunttu, Heidi M. T.; Runtuvuori-Salmela, Anniina; Middelboe, Mathias; Clark, Jason; Sundberg, Lotta-Riina (MDPI AG, 2021)Viruses of bacteria, bacteriophages, specifically infect their bacterial hosts with minimal effects on the surrounding microbiota. They have the potential to be used in the prevention and treatment of bacterial infections, ... -
Antibacterial efficiency of surface-immobilized Flavobacterium-infecting bacteriophage
Leppänen, Miika; Maasilta, Ilari; Sundberg, Lotta-Riina (American Chemical Society, 2019)Control of bacterial diseases by bacteriophages (phages) is gaining more interest due to increasing antibiotic resistance. This has led to technologies to attach phages on surfaces to form a biomaterial that can functionally ... -
Bacteriophage Adherence to Mucus Mediates Preventive Protection against Pathogenic Bacteria
Almeida, Gabriel M. F.; Laanto, Elina; Ashrafi, Roghaleh; Sundberg, Lotta-Riina (American Society for Microbiology, 2019)Metazoans were proposed to host bacteriophages on their mucosal surfaces in a symbiotic relationship, where phages provide an external immunity against bacterial infections and the metazoans provide phages a medium for ... -
Outside-host phage therapy as a biological control against environmental infectious diseases
Merikanto, Ilona; Laakso, Jouni; Kaitala, Veijo (BioMed Central, 2018)Background Environmentally growing pathogens present an increasing threat for human health, wildlife and food production. Treating the hosts with antibiotics or parasitic bacteriophages fail to eliminate diseases that ... -
Prevalence of genetically similar Flavobacterium columnare phages across aquaculture environments reveals a strong potential for pathogen control
Runtuvuori‐Salmela, A.; Kunttu, H. M. T.; Laanto, E.; Almeida, G. M. F.; Mäkelä, K.; Middelboe, M.; Sundberg, L‐R. (Wiley-Blackwell, 2022)Intensive aquaculture conditions expose fish to bacterial infections, leading to significant financial losses, extensive antibiotic use and risk of antibiotic resistance in target bacteria. Flavobacterium columnare causes ...