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dc.contributor.authorLeppänen, Miika
dc.date.accessioned2020-06-11T05:45:45Z
dc.date.available2020-06-11T05:45:45Z
dc.date.issued2020
dc.identifier.isbnISBN 978-951-39-8187-7
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/69863
dc.description.abstractIn this thesis, Helium Ion Microscopy (HIM) imaging and milling on organic and antibacterial materials will be discussed. In addition, I will discuss the antibacterial properties of surface-immobilized bacteriophages. HIM is a recently developed imaging method, which is especially suitable for biological samples because they can be imaged without a metal coating. Because of the state-of-the-art ion source, the microscope has also a higher imaging resolution compared to the scanning electron microscope (SEM). The suitability of HIM imaging for the bacteria-phage interactions is discussed in addition to the more delicate nanocellulose samples. High beam damage on the cellulose was found which has not been reported previously with HIM. Indeed an ion beam can have a milling property, which is demonstrated here with bacteria, bacteria-dragonfly interactions and encapsulated bacteriophages to obtain volumetric information. The antibacterial effectivity of immobilized bacteriophages was analyzed quantitatively with HIM-imaging and biological measures. It was found that detaching phages from the material are likely the major contributor to the infectivity of the phage-biomaterial.en
dc.relation.ispartofseriesJYU dissertations
dc.relation.haspart<b>Artikkeli I:</b> Leppänen, Miika; Maasilta, Ilari; Sundberg, Lotta-Riina (2019). Antibacterial efficiency of surface-immobilized Flavobacterium-infecting bacteriophage. <i>ACS Applied Bio Materials, 2 (11), 4720-4727.</i> <a href="https://doi.org/10.1021/acsabm.9b00242"target="_blank"> DOI: 10.1021/acsabm.9b00242</a>
dc.relation.haspart<b>Artikkeli II:</b> Leppänen, M., Sundberg, L.-R., Laanto, E., De Freitas Almeida, G., Papponen, P., & Maasilta, I. (2017). Imaging Bacterial Colonies and Phage-Bacterium Interaction at Sub-Nanometer Resolution Using Helium-Ion Microscopy. <i>Advanced Biosystems, 1 (8), 1700070.</i> <a href="https://doi.org/10.1002/adbi.201700070"target="_blank"> DOI: 10.1002/adbi.201700070</a>
dc.relation.haspart<b>Artikkeli III:</b> Ketola, A. E., Leppänen, M., Turpeinen, T., Papponen, P., Strand, A., Sundberg, A., . . . , & Retulainen, E. (2019). Cellulose nanofibrils prepared by gentle drying methods reveal the limits of helium ion microscopy imaging. <i>RSC Advances, 9 (27), 15668-15677.</i> <a href="https://doi.org/10.1039/C9RA01447K"target="_blank"> DOI: 10.1039/C9RA01447K</a>
dc.relation.haspart<b>Artikkeli IV:</b> Vinner, G. K., Rezaie-Yazdi, Z., Leppänen, M., Stapley, A. G. F., Leaper, M. C., & Malik, D. J. (2019). Microencapsulation of Salmonella-Specific Bacteriophage Felix O1 Using Spray-Drying in a pH-Responsive Formulation and Direct Compression Tableting of Powders into a Solid Oral Dosage Form. <i>Pharmaceuticals, 12 (1), 43.</i> <a href="https://doi.org/10.3390/ph12010043"target="_blank"> DOI: 10.3390/ph12010043</a>
dc.relation.haspart<b>Artikkeli V:</b> Vinner, Gurinder K.; Richards, Kerry; Leppänen, Miika; Sagona, Antonia P.; Malik 1, Danish J. (2019). Microencapsulation of Enteric Bacteriophages in a pH-Responsive Solid Oral Dosage Formulation Using a Scalable Membrane Emulsification Process. <i>Pharmaceutics, 11 (9), 475.</i> <a href="https://doi.org/10.3390/pharmaceutics11090475"target="_blank"> DOI: 10.3390/pharmaceutics11090475</a>
dc.relation.haspart<b>Artikkeli VI:</b> Bandara, Chaturanga D.; Ballerin, Giulia; Leppänen, Miika; Tesfamichael, Tuquabo; Ostrikov, Kostya (Ken); Whitchurch, Cynthia B. (2020). Resolving Bio-Nano Interactions of E.coli Bacteria-Dragonfly Wing Interface with Helium Ion and 3D-Structured Illumination Microscopy to Understand Bacterial Death on Nanotopography. <i>ACS Biomaterials Science & Engineering, 6 (7), 3925-3932.</i> <a href="https://doi.org/10.1021/acsbiomaterials.9b01973"target="_blank"> DOI: 10.1021/acsbiomaterials.9b01973</a>
dc.relation.haspart<b>Artikkeli VII:</b> De Freitas Almeida, G., Leppänen, M., Maasilta, I., & Sundberg, L.-R. (2018). Bacteriophage imaging : past, present and future. <i>Research in Microbiology, 169 (9), 488-494.</i> <a href="https://doi.org/10.1016/j.resmic.2018.05.006"target="_blank"> DOI: 10.1016/j.resmic.2018.05.006</a>
dc.subjectmikroskopia
dc.subjectfokusoidut ionisuihkut
dc.subjectbiomateriaalit
dc.subjectbakteriofagit
dc.subjectfagiterapia
dc.subjectnanoselluloosa
dc.subjectmateriaalitutkimus
dc.subjectheliumionimikroskopia
dc.subjecthelium ion microscopy
dc.subjectfocused ion beam milling
dc.subjectnanocellulose
dc.subjectphage therapy
dc.subjectphage-biomaterial
dc.subjectbacteriophage immobilization
dc.titleInfection under the ion beam : focused ion beams and antibacterial properties of biomaterials
dc.identifier.urnURN:ISBN:978-951-39-8187-7
dc.date.digitised


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