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dc.contributor.authorMäntylä, Elina
dc.contributor.authorAho, Vesa
dc.contributor.authorKann, Michael
dc.contributor.authorVihinen-Ranta, Maija
dc.date.accessioned2019-11-27T11:02:36Z
dc.date.available2019-11-27T11:02:36Z
dc.date.issued2020
dc.identifier.citationMäntylä, E., Aho, V., Kann, M., & Vihinen-Ranta, M. (2020). Cytoplasmic parvovirus capsids recruit importin beta for nuclear delivery. <i>Journal of Virology</i>, <i>94</i>(4), Article e01532-19. <a href="https://doi.org/10.1128/JVI.01532-19" target="_blank">https://doi.org/10.1128/JVI.01532-19</a>
dc.identifier.otherCONVID_33613134
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/66543
dc.description.abstractParvoviruses are an important platform for gene and cancer therapy. Their cell entry and the following steps including nuclear import are inefficient limiting their use in therapeutic applications. Two models exist on parvoviral nuclear entry: classical import of the viral capsid using nuclear transport receptors of the importin (karyopherin) family, or direct attachment of the capsid to the nuclear pore complex leading to local disintegration of the nuclear envelope. Here, by laser scanning confocal microscopy and in situ proximity ligation analysis combined with co-immunoprecipitation we showed that infection requires importin β-mediated access into the nuclear pore complex and nucleoporin 153-mediated interactions on the nuclear side. Importin β-capsid interaction continued within the nucleoplasm, which suggests that a mixed model of nuclear entry in which the classical nuclear import across the nuclear pore complex is accompanied by transient ruptures of the nuclear envelope allowing also passive entry of importin β-capsid complexes into the nucleus.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofseriesJournal of Virology
dc.rightsIn Copyright
dc.subject.otherParvovirus capsid
dc.subject.otherimportin β
dc.subject.otherinteraction
dc.subject.othercytoplasm
dc.subject.othernucleoplasm
dc.titleCytoplasmic parvovirus capsids recruit importin beta for nuclear delivery
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201911275033
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.description.reviewstatuspeerReviewed
dc.relation.issn0022-538X
dc.relation.numberinseries4
dc.relation.volume94
dc.type.versionacceptedVersion
dc.rights.copyright© 2019 American Society for Microbiology
dc.rights.accesslevelopenAccessfi
dc.subject.ysokaryoferiinit
dc.subject.ysotuma
dc.subject.ysoparvovirukset
dc.subject.ysosolulima
dc.subject.ysokapsidi
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p38410
jyx.subject.urihttp://www.yso.fi/onto/yso/p2411
jyx.subject.urihttp://www.yso.fi/onto/yso/p21764
jyx.subject.urihttp://www.yso.fi/onto/yso/p28857
jyx.subject.urihttp://www.yso.fi/onto/yso/p28020
dc.rights.urlhttp://rightsstatements.org/page/InC/1.0/?language=en
dc.relation.doi10.1128/JVI.01532-19
jyx.fundinginformationThis work was financed by the Academy of Finland under the award number 138388 (MVR), the Graduate School of the University of Jyvaskyla (EM), Finnish cultural foundation (EM) and Jane and Aatos Erkko Foundation (MVR)


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