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dc.contributor.authorLensu, S.
dc.contributor.authorPekkala, S. P.
dc.contributor.authorMäkinen, A.
dc.contributor.authorKarstunen, N.
dc.contributor.authorTurpeinen, A. T.
dc.contributor.authorHulmi, J.J.
dc.contributor.authorSilvennoinen, M.M.
dc.contributor.authorMa, H.
dc.contributor.authorKujala, U. M.
dc.contributor.authorKarvinen, S.
dc.contributor.authorKoch, L. G.
dc.contributor.authorBritton
dc.contributor.authorS., L.
dc.contributor.authorKainulainen, H.
dc.date.accessioned2019-10-28T12:32:33Z
dc.date.available2019-10-28T12:32:33Z
dc.date.issued2019
dc.identifier.citationLensu, S., Pekkala, S. P., Mäkinen, A., Karstunen, N., Turpeinen, A. T., Hulmi, J.J., Silvennoinen, M.M., Ma, H., Kujala, U. M., Karvinen, S., Koch, L. G., Britton, S., L., Kainulainen, H. (2019). Beneficial effects of running and milk protein supplements on Sirtuins and risk factors of metabolic disorders in rats with low aerobic capacity. <i>Metabolism Open</i>, <i>4</i>, Article 100019. <a href="https://doi.org/10.1016/j.metop.2019.100019" target="_blank">https://doi.org/10.1016/j.metop.2019.100019</a>
dc.identifier.otherCONVID_33250736
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/66091
dc.description.abstractBackground. Physical activity and dietary intake of dairy products are associated with improved metabolic health. Dairy products are rich with branched chain amino acids that are essential for energy production. To gain insight into the mechanisms underlying the benefit of the sub-chronic effects of running and intake of milk protein supplements, we studied Low Capacity Runner rats (LCR), a rodent exercise model with risk for metabolic disorders. We especially focused on the role of Sirtuins, energy level dependent proteins that affect many cellular metabolic processes. Methods. Forty-seven adult LCR female rats sedentary or running voluntarily in wheels were fed normal chow and given supplements of either whey or milk protein drink (PD)-supplemented water, or water only for 21 weeks. Physiological responses were measured in vivo. Blood lipids were determined from serum. Mitochondrial markers and Sirtuins (Sirt1-7) including downstream targets were measured in plantaris muscle by western blotting. Results. For the first 10 weeks whey-drinking rats ran about 50% less compared to other groups; still, in all runners glucose tolerance improved and triglycerides decreased. Generally, running induced a ∼six-fold increase in running capacity and a ∼8% decrease in % body fat. Together with running, protein supplements increased the relative lean mass of the total body weight by ∼11%. In comparison with sedentary controls, running and whey increased HDL (21%) and whey, with or without running, lowered LDL (-34%). Running increased mitochondrial biogenesis and Sirtuins 3 and 4. When combined with exercise, both whey and milk protein drink induced about a 4-fold increase in Sirt3, compared to runners drinking water only, and about a 2-fold increase compared to the respective sedentary group. Protein supplements, with or without running, enhanced the phosphorylation level of the acetyl-coA-carboxylase, suggesting increased fat oxidation. Both supplemented diets increased Sirt5 and Sirt7 without an additional effect from exercise. Running diminished and PD supplement increased Sirt6. Conclusion. We demonstrate in rats new sub-chronic effects of milk proteins on metabolism that involve Sirtuins and their downstream targets in skeletal muscle. The results show that running and milk proteins act on reducing the risk factors of metabolic disorders and suggest that the underlying mechanisms may involve Sirtuins. Notably, we find milk protein supplements have some favorable effects on metabolism even without running.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofseriesMetabolism Open
dc.rightsCC BY-NC-ND 4.0
dc.subject.otherlow capacity running rat (LCR)
dc.subject.othermetabolism
dc.subject.othermuscle
dc.subject.otherrunning
dc.subject.otherwhey
dc.titleBeneficial effects of running and milk protein supplements on Sirtuins and risk factors of metabolic disorders in rats with low aerobic capacity
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201910284652
dc.contributor.laitosLiikuntatieteellinen tiedekuntafi
dc.contributor.laitosFaculty of Sport and Health Sciencesen
dc.contributor.oppiaineLiikuntafysiologiafi
dc.contributor.oppiaineLiikuntalääketiedefi
dc.contributor.oppiaineExercise Physiologyen
dc.contributor.oppiaineSports and Exercise Medicineen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn2589-9368
dc.relation.volume4
dc.type.versionpublishedVersion
dc.rights.copyright© 2019 The Author(s)
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber275922
dc.subject.ysolihakset
dc.subject.ysoaineenvaihdunta
dc.subject.ysomaitovalmisteet
dc.subject.ysohera
dc.subject.ysomaitoproteiinit
dc.subject.ysosirtuiinit
dc.subject.ysoproteiinit
dc.subject.ysomeijerituotteet
dc.subject.ysojuoksu
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p2784
jyx.subject.urihttp://www.yso.fi/onto/yso/p3066
jyx.subject.urihttp://www.yso.fi/onto/yso/p4020
jyx.subject.urihttp://www.yso.fi/onto/yso/p15752
jyx.subject.urihttp://www.yso.fi/onto/yso/p29434
jyx.subject.urihttp://www.yso.fi/onto/yso/p29025
jyx.subject.urihttp://www.yso.fi/onto/yso/p4332
jyx.subject.urihttp://www.yso.fi/onto/yso/p8258
jyx.subject.urihttp://www.yso.fi/onto/yso/p9087
dc.rights.urlhttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.relation.doi10.1016/j.metop.2019.100019
dc.relation.funderSuomen Akatemiafi
dc.relation.funderResearch Council of Finlanden
jyx.fundingprogramAkatemiatutkija, SAfi
jyx.fundingprogramAcademy Research Fellow, AoFen
jyx.fundinginformationSatu Pekkala (#308042) and Juha Hulmi (#275922) are Research Fellows of the Academy of Finland. Contact LGK (Lauren.Koch2@UToledo.Edu) or SLB (brittons@umich.edu) for information on the LCR and HCR rats: these rat models are maintained as an international collaborative resource at The University of Toledo, Toledo, Ohio.
dc.type.okmA1


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