Label-free profiling of skeletal muscle using high-definition mass spectrometry
Burniston, J. G., Connolly, J., Kainulainen, H., Britton, S. L., & Koch, L. G. (2014). Label-free profiling of skeletal muscle using high-definition mass spectrometry. Proteomics, 14(20), 2339-2344. https://doi.org/10.1002/pmic.201400118
Published in
ProteomicsAuthors
Date
2014Copyright
© 2014 WILEY‐VCH Verlag GmbH & Co
We report automated and time‐efficient (2 h per sample) profiling of muscle using ultra‐performance LC coupled directly with high‐definition MS (HDMSE). Soluble proteins extracted from rat gastrocnemius (n = 10) were digested with trypsin and analyzed in duplicate using a 90 min RPLC gradient. Protein identification and label‐free quantitation were performed from HDMSE spectra analyzed using Progenesis QI for Proteomics software. In total 1514 proteins were identified. Of these, 811 had at least three unique peptides and were subsequently used to assess the dynamic range and precision of LC‐HDMSE label‐free profiling. Proteins analyzed by LC‐HDMSE encompass the entire complement of glycolytic, β‐oxidation, and tricarboxylic acid enzymes. In addition, numerous components of the electron transport chain and protein kinases involved in skeletal muscle regulation were detected. The dynamic range of protein abundances spanned four orders of magnitude. The correlation between technical replicates of the ten biological samples was R2 = 0.9961 ± 0.0036 (95% CI = 0.9940 – 0.9992) and the technical CV averaged 7.3 ± 6.7% (95% CI = 6.87 – 7.79%). This represents the most sophisticated label‐free profiling of skeletal muscle to date.
...
Publisher
Wiley-VCH VerlagISSN Search the Publication Forum
1615-9861Publication in research information system
https://converis.jyu.fi/converis/portal/detail/Publication/23916251
Metadata
Show full item recordCollections
- Liikuntatieteiden tiedekunta [3148]
License
Related items
Showing items with similar title or keywords.
-
Estrogenic regulation of skeletal muscle proteome : a study of premenopausal women and postmenopausal MZ cotwins discordant for hormonal therapy
Laakkonen, Eija; Soliymani, Rabah; Karvinen, Sira; Kaprio, Jaakko; Kujala, Urho; Baumann, Marc; Sipilä, Sarianna; Kovanen, Vuokko; Lalowski, Maciej (Wiley-Blackwell Publishing Ltd, 2017)Female middle age is characterized by a decline in skeletal muscle mass and performance, predisposing women to sarcopenia, functional limitations, and metabolic dysfunction as they age. Menopausal loss of ovarian function ... -
Inflammatory proteomics profiling for prediction of incident atrial fibrillation
Börschel, Christin S.; Ortega-Alonso, Alfredo; Havulinna, Aki S.; Jousilahti, Pekka; Salmi, Marko; Jalkanen, Sirpa; Salomaa, Veikko; Niiranen, Teemu; Schnabel, Renate B. (BMJ Publishing Group, 2023)Objective Atrial fibrillation (AF) has emerged as a common condition in older adults. Cardiovascular risk factors only explain about 50% of AF cases. Inflammatory biomarkers may help close this gap as inflammation can alter ... -
Neurological‐related proteomic profiling in plasma of children with metabolic healthy and unhealthy overweight/obesity
Olvera‐Rojas, Marcos; Plaza‐Florido, Abel; Solis‐Urra, Patricio; Osuna‐Prieto, Francisco J.; Ortega, Francisco B. (Wiley-Blackwell, 2024)Objective Children with overweight/obesity (OW/OB) exhibit poor cardiometabolic health, yet mechanisms influencing brain health remain unclear. We examined the differences in neurological-related circulating proteins in ... -
Learner’s beliefs : a case study of adult immigrants independently learning Finnish as a second language
Siren, Maricris (2021)Learners’ beliefs about second language acquisition (SLA) are described as learners’ personal theories about language learning and language in general. Previous studies reported that beliefs can be productive or unproductive ... -
Combinatorial and independent effects of exercise and myostatin/activin blocking on muscle gene expression profiling
Papaioannou, Konstantinos-Georgios G. (2013)The administration of soluble ligand binding domain of type IIb activin receptor fused to the Fc domain (sActRIIB-Fc) has been recently shown to attenuate dystrophic pathology and to increase muscle mass, but also to supress ...