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dc.contributor.authorKeskitalo, Anniina
dc.contributor.authorMunukka, Eveliina
dc.contributor.authorToivonen, Raine
dc.contributor.authorHollmén, Maija
dc.contributor.authorKainulainen, Heikki
dc.contributor.authorHuovinen, Pentti
dc.contributor.authorJalkanen, Sirpa
dc.contributor.authorPekkala, Satu
dc.date.accessioned2018-06-04T06:18:34Z
dc.date.available2018-06-04T06:18:34Z
dc.date.issued2018
dc.identifier.citationKeskitalo, A., Munukka, E., Toivonen, R., Hollmén, M., Kainulainen, H., Huovinen, P., Jalkanen, S., & Pekkala, S. (2018). Enterobacter cloacae administration induces hepatic damage and subcutaneous fat accumulation in high-fat diet fed mice. <i>PLoS ONE</i>, <i>13</i>(5), Article e0198262. <a href="https://doi.org/10.1371/journal.pone.0198262" target="_blank">https://doi.org/10.1371/journal.pone.0198262</a>
dc.identifier.otherCONVID_28076129
dc.identifier.otherTUTKAID_77761
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/58308
dc.description.abstractAccumulating evidence indicates that gut microbiota plays a significant role in obesity, insulin resistance and associated liver disorders. Family Enterobacteriaceae and especially Enterobacter cloacae strain B29 have been previously linked to obesity and hepatic damage. The underlying mechanisms, however, remain unclear. Therefore, we comprehensively examined the effects of E. cloacae subsp. cloacae (ATCC® 13047™) administration on host metabolism of mice fed with high-fat diet (HFD). C57BL/6N mice were randomly divided into HFD control, chow control, and E. cloacae treatment groups. The E. cloacae treatment group received live bacterial cells in PBS intragastrically twice a week, every other week for 13 weeks. Both control groups received PBS intragastrically. After the 13- week treatment period, the mice were sacrificed for gene and protein expression and functional analyses. Our results show that E. cloacae administration increased subcutaneous fat mass and the relative proportion of hypertrophic adipocytes. Both subcutaneous and visceral fat had signs of decreased insulin signaling and elevated lipolysis that was reflected in higher serum glycerol levels. In addition, E. cloacae -treated mice had significantly higher hepatic AST and AST/ALT ratio, and their liver histology indicated fibrosis, demonstrating that E. cloacae subsp. cloacae administration promotes hepatic damage in HFD fed mice.fi
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherPublic Library of Science
dc.relation.ispartofseriesPLoS ONE
dc.rightsCC BY 4.0
dc.subject.otherrasvat (orgaaniset yhdisteet)
dc.subject.otheradipose tissue
dc.subject.otherglycerol
dc.subject.otheradipocytes
dc.subject.otherlipolysis
dc.subject.otherdiet
dc.titleEnterobacter cloacae administration induces hepatic damage and subcutaneous fat accumulation in high-fat diet fed mice
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201805312963
dc.contributor.laitosLiikuntatieteellinen tiedekuntafi
dc.contributor.laitosFaculty of Sport and Health Sciencesen
dc.contributor.oppiaineLiikuntafysiologiafi
dc.contributor.oppiaineExercise Physiologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2018-05-31T12:15:15Z
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn1932-6203
dc.relation.numberinseries5
dc.relation.volume13
dc.type.versionpublishedVersion
dc.rights.copyright© 2018 Keskitalo et al.
dc.rights.accesslevelopenAccessfi
dc.subject.ysosuolistomikrobisto
dc.subject.ysoenterobakteerit
dc.subject.ysorasvakudokset
dc.subject.ysolihavuus
dc.subject.ysorasvahapot
dc.subject.ysoruokavaliot
dc.subject.ysorasvat
dc.subject.ysotulehdus
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p37925
jyx.subject.urihttp://www.yso.fi/onto/yso/p28554
jyx.subject.urihttp://www.yso.fi/onto/yso/p24382
jyx.subject.urihttp://www.yso.fi/onto/yso/p823
jyx.subject.urihttp://www.yso.fi/onto/yso/p4800
jyx.subject.urihttp://www.yso.fi/onto/yso/p3790
jyx.subject.urihttp://www.yso.fi/onto/yso/p4153
jyx.subject.urihttp://www.yso.fi/onto/yso/p1049
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1371/journal.pone.0198262
dc.type.okmA1


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