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dc.contributor.authorCheng, Sulin
dc.contributor.authorWiklund, Petri
dc.contributor.authorAutio, Reija
dc.contributor.authorBorra, Ronald
dc.contributor.authorOjanen, Xiaowei
dc.contributor.authorXu, Leiting
dc.contributor.authorTörmäkangas, Timo
dc.contributor.authorAlén, Markku
dc.date.accessioned2015-10-26T09:57:50Z
dc.date.available2015-10-26T09:57:50Z
dc.date.issued2015
dc.identifier.citationCheng, S., Wiklund, P., Autio, R., Borra, R., Ojanen, X., Xu, L., Törmäkangas, T., & Alén, M. (2015). Adipose Tissue Dysfunction and Altered Systemic Amino Acid Metabolism Are Associated with Non-Alcoholic Fatty Liver Disease. <i>PLoS ONE</i>, <i>10</i>(10), Article e0138889. <a href="https://doi.org/10.1371/journal.pone.0138889" target="_blank">https://doi.org/10.1371/journal.pone.0138889</a>
dc.identifier.otherCONVID_25244310
dc.identifier.otherTUTKAID_67510
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/47407
dc.description.abstractBackground Fatty liver is a major cause of obesity-related morbidity and mortality. The aim of this study was to identify early metabolic alterations associated with liver fat accumulation in 50- to 55- year-old men (n = 49) and women (n = 52) with and without NAFLD. Methods Hepatic fat content was measured using proton magnetic resonance spectroscopy (1 H MRS). Serum samples were analyzed using a nuclear magnetic resonance (NMR) metabolomics platform. Global gene expression profiles of adipose tissues and skeletal muscle were analyzed using Affymetrix microarrays and quantitative PCR. Muscle protein expression was analyzed by Western blot. Results Increased branched-chain amino acid (BCAA), aromatic amino acid (AAA) and orosomucoid were associated with liver fat accumulation already in its early stage, independent of sex, obesity or insulin resistance (p<0.05 for all). Significant down-regulation of BCAA catabolism and fatty acid and energy metabolism was observed in the adipose tissue of the NAFLD group (p<0.001for all), whereas no aberrant gene expression in the skeletal muscle was found. Reduced BCAA catabolic activity was inversely associated with serum BCAA and liver fat content (p<0.05 for all). Conclusions Liver fat accumulation, already in its early stage, is associated with increased serum branched-chain and aromatic amino acids. The observed associations of decreased BCAA catabolism activity, mitochondrial energy metabolism and serum BCAA concentration with liver fat content suggest that adipose tissue dysfunction may have a key role in the systemic nature of NAFLD pathogenesis.
dc.language.isoeng
dc.publisherPublic Library of Science
dc.relation.ispartofseriesPLoS ONE
dc.subject.otheradipose tissue
dc.subject.otheramino acid metabolism
dc.titleAdipose Tissue Dysfunction and Altered Systemic Amino Acid Metabolism Are Associated with Non-Alcoholic Fatty Liver Disease
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201510233481
dc.contributor.laitosTerveystieteiden laitosfi
dc.contributor.laitosDepartment of Health Sciencesen
dc.contributor.oppiaineGerontologia ja kansanterveysfi
dc.contributor.oppiaineLiikuntalääketiedefi
dc.contributor.oppiaineGerontology and Public Healthen
dc.contributor.oppiaineSports and Exercise Medicineen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2015-10-23T12:15:17Z
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn1932-6203
dc.relation.numberinseries10
dc.relation.volume10
dc.type.versionpublishedVersion
dc.rights.copyright© 2015 Cheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.accesslevelopenAccessfi
dc.subject.ysorasvamaksa
jyx.subject.urihttp://www.yso.fi/onto/yso/p21481
dc.rights.urlhttp://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1371/journal.pone.0138889
dc.type.okmA1


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© 2015 Cheng et al. This is an open
access article distributed under the terms of the
Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any
medium, provided the original author and source are
credited.
Except where otherwise noted, this item's license is described as © 2015 Cheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.