An in vitro model to study the adipose tissue toll-like receptor -mediated effects of gut microbiota on non-alcoholic fatty liver disease development
Tekijät
Päivämäärä
2017Alkoholista riippumaton rasvamaksa (NAFLD) on maailman yleisin maksasairaus ja saattaa johtaa kirroosin tai maksasyövän kehittymiseen. Viimeaikaisissa tutkimuksissa suolistosta peräisin olevin bakteerimolekyylien, kuten flagelliinin (FLG) ja lipopolysakkaridien (LPS), rooli rasvamaksan kehittymisessä on tunnistettu. Nämä molekyylit saattavat vaikuttaa maksaan välillisesti rasvakudoksen kautta toll-like reseptorien (TLR) signaalireittejä aktivoiden. Tässä tutkimuksessa selvitettiin FLG:n ja LPS:n rasvasoluvälitteisiä vaikutuksia HepG2-hepatoomasoluihin. Hypoteesina oli, että FLG ja LPS lisäisivät HepG2 soluissa rasvan kerääntymistä, insuliiniresistenssiä ja tulehdusta, joita mitattiin kvantitatiivisella PCR:llä sekä western blotilla. Tulokset osoittavat, että FLG saattaa vaikuttaa rasvamaksan kehitykseen erityisesti adiposyyttien kautta. Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease in the world, and may lead to development of cirrhosis or hepatocellular carcinoma. Lately the role of molecules from gut-derived bacteria, like flagellin (FLG) and lipopolysaccharides (LPS) in development of NAFLD has been recognized. These molecules could affect the liver indirectly through activation of adipose tissue toll-like receptor (TLR) pathways. In this study the potential adipocyte mediated effects of FLG and LPS on HepG2 hepatoma cells were explored. The hypothesis was that FLG and LPS would increase HepG2 fat accumulation, insulin resistance and inflammation measured with quantitative realtime PCR (qPCR) and western blot. According to the results especially adipocyte mediated FLG could have a role in fatty liver development.
Metadata
Näytä kaikki kuvailutiedotKokoelmat
- Pro gradu -tutkielmat [29533]
Samankaltainen aineisto
Näytetään aineistoja, joilla on samankaltainen nimeke tai asiasanat.
-
Fecal Metagenomics and Metabolomics Identifying Microbial Signatures in Non-Alcoholic Fatty Liver Disease
Pekkala, Satu (MDPI AG, 2023)The frequency of non-alcoholic fatty liver disease (NAFLD) has intensified, creating diagnostic challenges and increasing the need for reliable non-invasive diagnostic tools. Due to the importance of the gut–liver axis in ... -
Adipose Tissue Dysfunction and Altered Systemic Amino Acid Metabolism Are Associated with Non-Alcoholic Fatty Liver Disease
Cheng, Sulin; Wiklund, Petri; Autio, Reija; Borra, Ronald; Ojanen, Xiaowei; Xu, Leiting; Törmäkangas, Timo; Alén, Markku (Public Library of Science, 2015)Background Fatty liver is a major cause of obesity-related morbidity and mortality. The aim of this study was to identify early metabolic alterations associated with liver fat accumulation in 50- to 55- year-old men (n ... -
Effect of aerobic exercise and low carbohydrate diet on pre-diabetic non-alcoholic fatty liver disease in postmenopausal women and middle aged men: study protocol for the AELC randomized controlled trial
Liu, Wu Yi; Lu, Da Jiang; Du, Xia Ming; Sun, Jian Qin; Ge, Jun; Wang, Ren Wei; Wang, Ru; Zou, Jun; Xu, Chang; Ren, Jie; Wen, Xin Fei; Liu, Yang; Cheng, Shumei; Tan, Xiao; Pekkala, Satu; Munukka, Eveliina; Wiklund, Petri; Chen, Yan Qiu; Gu, Qing; Xia, Zheng Chang; Liu, Jun Jun; Liu, Wen Bin; Chen, Xue Bo; Zhang, Yi Min; Li, Rui; Borra, Ronald J H; Yao, Jia Xin; Chen, Pei Jie; Cheng, Sulin (BioMed Central, 2014)Background. Pre-diabetes and non-alcoholic fatty liver disease (NAFLD) are associated with an unhealthy lifestyle and pose extremely high costs to the healthcare system. In this study, we aim to explore whether individualized ... -
Effect of a Multicomponent Intervention on Hepatic Steatosis Is Partially Mediated by the Reduction of Intermuscular Abdominal Adipose Tissue in Children With Overweight or Obesity : The EFIGRO Project
Cadenas-Sanchez, Cristina; Idoate, Fernando; Cabeza, Rafael; Villanueva, Arantxa; Rodríguez-Vigil, Beatriz; Medrano, María; Osés, Maddi; Ortega, Francisco B.; Ruiz, Jonatan R.; Labayen, Idoia (American Diabetes Association, 2022)OBJECTIVE In adults, there is evidence that improvement of metabolic-associated fatty liver disease (MAFLD) depends on the reduction of myosteatosis. In children, in whom the prevalence of MAFLD is alarming, this muscle-liver ... -
The preventive role of gut microbiota modulating prebiotic xylo-oligosaccharides in non-alcoholic fatty liver disease
Honkanen, Marjaana (2020)Suolistomikrobiston koostumus on yhdistetty ei-alkoholiperäiseen rasvamaksatautiin (NAFLD). Erityisesti Faecalibacterium prausnitzii -bakteerin runsas määrä suolistossa voi edistää maksan terveyttä. Koska tätä bakteeria ...
Ellei toisin mainittu, julkisesti saatavilla olevia JYX-metatietoja (poislukien tiivistelmät) saa vapaasti uudelleenkäyttää CC0-lisenssillä.