Haku
Aineistot 1-10 / 21
Predictive value of p53, Ki67 and TLR5 in neoplastic progression of Barrett’s esophagus : a matched case-control study
(Springer, 2022)
Barrett’s esophagus progresses to high-grade dysplasia or cancer along the well-established metaplasia-dysplasia-adenocarcinoma sequence. The aim of this study was to evaluate the value of p53, Ki67, and toll-like receptor ...
Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells
(MDPI AG, 2022)
Like many bacterial species, Borrelia burgdorferi, the pleomorphic bacterium that causes Lyme borreliosis, produces outer membrane vesicles (OMVs). Borrelial OMVs (BbOMVs) have been identified as containing virulence ...
Immune tolerance disruption by human parvovirus B19 viral infection mechanisms
(2013)
. Kootuista apoptoottisista kappaleista immunoleimattiin tumaperäiset antigeenit apolipoproteiini H (ApoH), histoni 4 (H4), histoni 2B (H2B), lysosomiin liittyvä kalvoproteiini 2 (Lamp2), Ku80 ja Smith. Kaikki tumaperäiset antigeenit, poissulkien H2B...
There are several factors, of which viral infections are increasingly regarded as the greatest environmental cause of autoimmunity, influencing the development of an autoimmune disease. For example human parvovirus B19 (B19V) has been previously connected to systemic lupus erythematosus and rheumatic arthritis. It has also been shown that nonstructural protein 1 (NS1) of B19V induces apoptosis in cells not normally permissive for B19V infections. In addition, it has been recently demonstrated that apoptotic bodies resulting from apoptosis can present antigens to antigen presenting cells (APCs) such as macrophages or dendritic cells. The aim of this study is to detect self antigens related to B19V infections and study the immune response caused by NS1. For that, the NS1 has been fused with enhanced green fluorescent protein (EGFP) and incorporated into baculovirus vector under cytomegalovirus immediate early promoter. These recombinant baculovirus particles were amplified in Sf9 insect cell line. The resulting virus was then used to transduce HepG2 human hepatocytic cells. The transduction efficiency of the virus was measured with flow cytometry, employing the presence of the EGFP as a sign of transduction. Based on the transduction efficiency, a volume providing 70 % transduction efficiency of the virus stock was used to produce apoptotic bodies. These apoptotic bodies were first purified by filtering and then pelleted by ultracentrifugation. Collected apoptotic bodies were immunolabeled using nuclear antigens apolipoprotein H (ApoH), histone 4 (H4), histone 2B (H2B), lysosomal-associated membrane protein 2 (Lamp2), Ku80 and Smith. The selected nuclear antigens as well as NS1 were detected from the apoptotic bodies, except for H2B. The presence of nuclear antigens in the apoptotic bodies is a potential method for immune tolerance disruption which was then addressed by utilizing APCs. The APCs of choice were acute monocytic leukemia derived human monocytes (THP-1) due to their close resemblance to peripheral blood mononuclear cells. THP-1 cells were differentiated using phorbol-12-myristate-13-acetate (PMA). The cells were incubated in medium containing PMA for three days and then five more days in fresh medium to ensure proper differentiation. Differentiated THP-1 cells (dTHP-1) were next fed with purified apoptotic bodies. Internalization of B19V NS1 induced apoptotic bodies was observed using confocal microscopy. Since the APCs were capable of internalizing apoptotic bodies, the following cytokine profile was tentatively studied. The examination of possible immunological effects derived to a conclusion that only the presence of interferon γ inducible protein 10 (IP-10) separated the EGFP or EGFP-NS1 fusion protein containing samples from the control conditions. Although the underlying mechanisms leading into immune tolerance disruption by B19V infection mechanisms warrants further investigation, the data here supports the hypothesis that B19V NS1 induced apoptotic bodies from nonpermissive cells contain self-antigens which could be introduced to the immune system though APCs....
There are several factors, of which viral infections are increasingly regarded as the greatest environmental cause of autoimmunity, influencing the development of an autoimmune disease. For example human parvovirus B19 (B19V) has been previously connected to systemic lupus erythematosus and rheumatic arthritis. It has also been shown that nonstructural protein 1 (NS1) of B19V induces apoptosis in cells not normally permissive for B19V infections. In addition, it has been recently demonstrated that apoptotic bodies resulting from apoptosis can present antigens to antigen presenting cells (APCs) such as macrophages or dendritic cells. The aim of this study is to detect self antigens related to B19V infections and study the immune response caused by NS1. For that, the NS1 has been fused with enhanced green fluorescent protein (EGFP) and incorporated into baculovirus vector under cytomegalovirus immediate early promoter. These recombinant baculovirus particles were amplified in Sf9 insect cell line. The resulting virus was then used to transduce HepG2 human hepatocytic cells. The transduction efficiency of the virus was measured with flow cytometry, employing the presence of the EGFP as a sign of transduction. Based on the transduction efficiency, a volume providing 70 % transduction efficiency of the virus stock was used to produce apoptotic bodies. These apoptotic bodies were first purified by filtering and then pelleted by ultracentrifugation. Collected apoptotic bodies were immunolabeled using nuclear antigens apolipoprotein H (ApoH), histone 4 (H4), histone 2B (H2B), lysosomal-associated membrane protein 2 (Lamp2), Ku80 and Smith. The selected nuclear antigens as well as NS1 were detected from the apoptotic bodies, except for H2B. The presence of nuclear antigens in the apoptotic bodies is a potential method for immune tolerance disruption which was then addressed by utilizing APCs. The APCs of choice were acute monocytic leukemia derived human monocytes (THP-1) due to their close resemblance to peripheral blood mononuclear cells. THP-1 cells were differentiated using phorbol-12-myristate-13-acetate (PMA). The cells were incubated in medium containing PMA for three days and then five more days in fresh medium to ensure proper differentiation. Differentiated THP-1 cells (dTHP-1) were next fed with purified apoptotic bodies. Internalization of B19V NS1 induced apoptotic bodies was observed using confocal microscopy. Since the APCs were capable of internalizing apoptotic bodies, the following cytokine profile was tentatively studied. The examination of possible immunological effects derived to a conclusion that only the presence of interferon γ inducible protein 10 (IP-10) separated the EGFP or EGFP-NS1 fusion protein containing samples from the control conditions. Although the underlying mechanisms leading into immune tolerance disruption by B19V infection mechanisms warrants further investigation, the data here supports the hypothesis that B19V NS1 induced apoptotic bodies from nonpermissive cells contain self-antigens which could be introduced to the immune system though APCs....
Pathogenic mechanisms of how human parvovirus breaks self-tolerance
(University of Jyväskylä, 2017)
It is known that viral infections can cause acute, chronic, and autoimmune diseases
(ADs). However, the mechanism of how a persistent viral infection contributes to ADs
remains still unclear. In this thesis, pathological ...
TCF7-transkriptiotekijän merkitys T-auttajasolujen erilaistumisessa
(2009)
epiteelin soluilta. Usean kehitysvaiheen aikana soluista valitaan ne,
jotka tunnistavat vieraat antigeenit, eivätkä sitoudu liian voimakkaasti omien solujen rakenteisiin. Kypsyttyään naiivit T-auttajasolut (Th) vapautetaan elimistöön, jossa ne antigeeninsä...
Kuivaveren valkoisuus ja kliiniset verimuuttujat
(2014)
Tutkimuksen tarkoituksena oli selvittää kuivaverianalyysin yhteyksiä kliinisiin verimuuttujiin,
erityisesti laskoon. Lisäksi menetelmän luotettavuutta arvioitiin vertailemalla samalta
koehenkilöltä kerättyjä A- ja ...
Kestävyysharjoittelujakson vaikutukset syljen immunologisiin muuttujiin ja sairastuvuuteen
(2014)
Johdanto. Ylähengitystieinfektiot ovat yleisesti tunnettu ongelma niin urheilijoiden, kuin myös kuntoilijoiden keskuudessa. Etenkin kilpaurheilijoille terveenä pysyminen on erittäin tärkeää, jotta vältytään harjoittelua ...
Salmonella-bakteerin diagnostiikkamenetelmien soveltuvuus alkutuotannon näytematriiseille ja biofilmin muodostus eri serotyypeillä
(2022)
Salmonella-bakteerin esiintyvyyttä tuotantotiloilla, tuotantoeläimissä sekä elintarvikkeissa valvoo Suomessa kansallinen Salmonellavalvontaohjelma. ISO 6579-1 standardin mukainen bakteeriviljely on ollut toistaiseksi ainoa ...
Valkosolu- ja verihiutalevasteet voimaharjoituksen ja voimaharjoittelun yhteydessä miehillä: erityisesti proteiinin ja ikääntymisen vaikutukset
(2010)
Mutanen, Niina 2009. Valkosolu- ja verihiutalevasteet voimaharjoituksen ja voimaharjoittelun yhteydessä miehillä: erityisesti proteiinin ja ikääntymisen vaikutukset. Valmennus- ja testausopin kandidaatintutkielma. ...