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dc.contributor.authorThoral, Elisa
dc.contributor.authorGarcía-Díaz, Carmen C.
dc.contributor.authorPersson, Elin
dc.contributor.authorChamkha, Imen
dc.contributor.authorElmér, Eskil
dc.contributor.authorRuuskanen, Suvi
dc.contributor.authorNord, Andreas
dc.date.accessioned2024-04-09T10:03:35Z
dc.date.available2024-04-09T10:03:35Z
dc.date.issued2024
dc.identifier.citationThoral, E., García-Díaz, C. C., Persson, E., Chamkha, I., Elmér, E., Ruuskanen, S., & Nord, A. (2024). The relationship between mitochondrial respiration, resting metabolic rate and blood cell count in great tits. <i>Biology Open</i>, <i>13</i>(3). <a href="https://doi.org/10.1242/bio.060302" target="_blank">https://doi.org/10.1242/bio.060302</a>
dc.identifier.otherCONVID_207404190
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/94222
dc.description.abstractAlthough mitochondrial respiration is believed to explain a substantial part of the variation in resting metabolic rate (RMR), few studies have empirically studied the relationship between organismal and cellular metabolism. We therefore investigated the relationship between RMR and mitochondrial respiration of permeabilized blood cells in wild great tits (Parus major L.). We also studied the correlation between mitochondrial respiration traits and blood cell count, as normalizing mitochondrial respiration by the cell count is a method commonly used to study blood metabolism. In contrast to previous studies, our results show that there was no relationship between RMR and mitochondrial respiration in intact blood cells (i.e., with the ROUTINE respiration). However, when cells were permeabilised and interrelation re-assessed under saturating substrate availability, we found that RMR was positively related to phosphorylating respiration rates through complexes I and II (i.e., OXPHOS respiration) and to the mitochondrial efficiency to produce energy (i.e., Net phosphorylating efficiency), though variation explained by the models was low (i.e., linear model: R2=0.14 to 0.21). However, unlike studies in mammals, LEAK respiration without (i.e., L(n)) and with (i.e., L(Omy)) adenylates was not significantly related to RMR. These results suggest that phosphorylating respiration in blood cells can potentially be used to predict RMR in wild birds, but that this relationship may have to be addressed in standardized conditions (permeabilized cells) and that the prediction risks being imprecise. We also showed that, in our conditions, there was no relationship between any mitochondrial respiration trait and blood cell count. Hence, we caution against normalising respiration rates using this parameter as is sometimes done. Future work should address the functional explanations for the observed relationships, and determine why these appear labile across space, time, taxon, and physiological state.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherThe Company of Biologists
dc.relation.ispartofseriesBiology Open
dc.rightsCC BY 4.0
dc.subject.othererythrocyte
dc.subject.otheroxidative metabolism
dc.subject.otherresting metabolic rate
dc.subject.otherbasal metabolic rate
dc.subject.othergreat tit
dc.subject.othermitochondria
dc.titleThe relationship between mitochondrial respiration, resting metabolic rate and blood cell count in great tits
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202404092789
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn2046-6390
dc.relation.numberinseries3
dc.relation.volume13
dc.type.versionpublishedVersion
dc.rights.copyright© 2024. Published by The Company of Biologists Ltd.
dc.rights.accesslevelopenAccessfi
dc.subject.ysomitokondriot
dc.subject.ysotalitiainen
dc.subject.ysosoluhengitys
dc.subject.ysosolufysiologia
dc.subject.ysoaineenvaihdunta
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p21158
jyx.subject.urihttp://www.yso.fi/onto/yso/p12931
jyx.subject.urihttp://www.yso.fi/onto/yso/p24868
jyx.subject.urihttp://www.yso.fi/onto/yso/p25367
jyx.subject.urihttp://www.yso.fi/onto/yso/p3066
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1242/bio.060302
jyx.fundinginformationThis study was funded by the Swedish Research Council (2020-04686) and the Royal Physiographic Society of Lund (2017-39034, 2019-41011) (to AN). EP was supported by the Biology Open Crafoord Foundation (nos. 118290 and 121683) and ET was supported by a postdoctoral grant from the Carl Trygger Foundation for Scientific Research (CTS21-1173).
dc.type.okmA1


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