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dc.contributor.authorFachada, Vasco
dc.contributor.authorSilvennoinen, Mika
dc.contributor.authorSahinaho, Ulla-Maria
dc.contributor.authorRahkila, Paavo
dc.contributor.authorKivelä, Riikka
dc.contributor.authorHulmi, Juha J.
dc.contributor.authorKujala, Urho
dc.contributor.authorKainulainen, Heikki
dc.date.accessioned2023-02-23T13:34:59Z
dc.date.available2023-02-23T13:34:59Z
dc.date.issued2023
dc.identifier.citationFachada, V., Silvennoinen, M., Sahinaho, U.-M., Rahkila, P., Kivelä, R., Hulmi, J. J., Kujala, U., & Kainulainen, H. (2023). Effects of Long-Term Physical Activity and BCAA Availability on the Subcellular Associations between Intramyocellular Lipids, Perilipins and PGC-1α. <i>International Journal of Molecular Sciences</i>, <i>24</i>(5), Article 4282. <a href="https://doi.org/10.3390/ijms24054282" target="_blank">https://doi.org/10.3390/ijms24054282</a>
dc.identifier.otherCONVID_177036454
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/85621
dc.description.abstractCellular skeletal muscle lipid metabolism is of paramount importance for metabolic health, specifically through its connection to branched-chain amino acids (BCAA) metabolism and through its modulation by exercise. In this study, we aimed at better understanding intramyocellular lipids (IMCL) and their related key proteins in response to physical activity and BCAA deprivation. By means of confocal microscopy, we examined IMCL and the lipid droplet coating proteins PLIN2 and PLIN5 in human twin pairs discordant for physical activity. Additionally, in order to study IMCLs, PLINs and their association to peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α�) in cytosolic and nuclear pools, we mimicked exercise-induced contractions in C2C12 myotubes by electrical pulse stimulation (EPS), with or without BCAA deprivation. The life-long physically active twins displayed an increased IMCL signal in type I fibers when compared to their inactive twin pair. Moreover, the inactive twins showed a decreased association between PLIN2 and IMCL. Similarly, in the C2C12 cell line, PLIN2 dissociated from IMCL when myotubes were deprived of BCAA, especially when contracting. In addition, in myotubes, EPS led to an increase in nuclear PLIN5 signal and its associations with IMCL and PGC-1α�. This study demonstrates how physical activity and BCAA availability affects IMCL and their associated proteins, providing further and novel evidence for the link between the BCAA, energy and lipid metabolisms.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofseriesInternational Journal of Molecular Sciences
dc.rightsCC BY 4.0
dc.subject.otherlipid droplets
dc.subject.otherPLIN2
dc.subject.otherPLIN5
dc.subject.otherskeletal muscle
dc.subject.otherphysical activity
dc.subject.otherC2C12
dc.subject.otherelectrical pulse stimulation
dc.subject.otherEPS
dc.subject.othersubcellular localization
dc.titleEffects of Long-Term Physical Activity and BCAA Availability on the Subcellular Associations between Intramyocellular Lipids, Perilipins and PGC-1α
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202302231885
dc.contributor.laitosLiikuntatieteellinen tiedekuntafi
dc.contributor.laitosFaculty of Sport and Health Sciencesen
dc.contributor.oppiaineLiikuntafysiologiafi
dc.contributor.oppiaineLiikuntalääketiedefi
dc.contributor.oppiaineExercise Physiologyen
dc.contributor.oppiaineSports and Exercise Medicineen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn1661-6596
dc.relation.numberinseries5
dc.relation.volume24
dc.type.versionpublishedVersion
dc.rights.copyright© 2023 by the authors. Licensee MDPI, Basel, Switzerland.
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber298875
dc.subject.ysoproteiinit
dc.subject.ysofyysinen aktiivisuus
dc.subject.ysoterveysvaikutukset
dc.subject.ysoaineenvaihdunta
dc.subject.ysoaminohapot
dc.subject.ysoliikunta
dc.subject.ysolipidit
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p4332
jyx.subject.urihttp://www.yso.fi/onto/yso/p23102
jyx.subject.urihttp://www.yso.fi/onto/yso/p15449
jyx.subject.urihttp://www.yso.fi/onto/yso/p3066
jyx.subject.urihttp://www.yso.fi/onto/yso/p9530
jyx.subject.urihttp://www.yso.fi/onto/yso/p916
jyx.subject.urihttp://www.yso.fi/onto/yso/p4799
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.3390/ijms24054282
dc.relation.funderResearch Council of Finlanden
dc.relation.funderSuomen Akatemiafi
jyx.fundingprogramAcademy Project, AoFen
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundinginformationThis study was supported by the Academy of Finland (grants 132987, 298875 and 114866). The author V.F. acknowledges his FCT grant SFRH/BD/68308/2010.
dc.type.okmA1


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