Effects of exercise and genetics on skeletal muscle lipid metabolism with focus on the localization and association of lipid droplets, mitochondria ja PLIN5
This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.
Low exercise capacity has been identified as a stronger predictor of morbidity and mortality relative to other commonly reported risk factors. Substrate metabolism, especially lipid storage, lipolysis and transportation in the skeletal muscles is strongly linked to aerobic capacity. The physical topographical properties of some myocellular particles and or organelles such mitochondria, lipid droplets (LDs) and perilipine-5 (Plin5) proteins in the skeletal muscle tissues have been implicated in the onset of most metabolic diseases and may also predict overall physical capacity. We tested the effect of innate high aerobic capacity (genetics) and acquired aerobic capacity (voluntary running) on the number, location and colocalization of the aforementioned particles in the gastrocnemius muscle cells, between the low and high capacity runner rats. Muscle samples from the gastrocnemius muscle of the specially bred rat model with high aerobic capacity (HCR) (n=20) and low aerobic capacity (LCR) (n=20) were used in this study. The samples were obtained from 4 equal subgroups; high capacity runner control (HCR-C) (n=10), high capacity runner receiving exercise intervention (HCR-R) (n=10), low capacity runner control (LCR-C) (n=10) and low capacity runner receiving exercise intervention (LCR-R) (n=10). Immunocytochemistry, confocal microscopy and bioinformatics were used in data collections and analysis. A one-way between subject analysis of variance (ANOVA) show statistically significant difference in the content of mitochondria (F (3,24) = 4.24, p<.0.05) and PLIN5 particles (F (3,24) = 3.8, p<.005). There were no significant differences in the number of LD (F (3,24) = 0.43, p>0.05) or in the colocalization of the LD and PLIN5 particles (F (3,24) = 1.2, p>.005) and LD and COXIV particles (F (3,24) = 1, p>.005) across the groups. The result suggests a strong interplay between genetic background and exercise stimulus. These factors may influence the skeletal muscle properties at the cellular level, effect of which may not only affect performance component but also health component of physical fitness. Physical activity may, however, enhance the cellular function or reverse genetic lags in cellular properties. ...
MetadataShow full item record
- Pro gradu -tutkielmat 
Showing items with similar title or keywords.
Comparison of acute and chronic exercise effects in the lipid droplets topography skeletal muscle, following high and low-fat diet in mice Triantou, Vasiliki (2015)In the modern world, diet patterns high on lipids, sedentary lifestyle and obesity contribute all in the development of metabolic syndrome, which in turn can lead to type 2 diabetes. All the excess fats, that the majority ...
Genetic and Environmental Effects on the Individual Variation and Continuity of Participation in Diverse Physical Activities Kaartinen, Sara; Silventoinen, Karri; Korhonen, Tellervo; Kujala, Urho M.; Kaprio, Jaakko; Aaltonen, Sari (Lippincott Williams & Wilkins, 2021)Introduction Participation in diverse physical activities has beneficial health effects. However, little is known on how genetic and environmental factors affect this trait. Thus, we examined to what extent these factors ...
Aaltonen, Sari; Palviainen, Teemu; Rose, Richard J.; Kujala, Urho M.; Kaprio, Jaakko; Silventoinen, Karri (Human Kinetics Publishers, 2021)Background: Both genetic and environmental influences have been shown to contribute to the association between physical activity and overall academic performance. The authors examined whether leisure-time physical activity ...
Pakkala, Inka (University of Jyväskylä, 2012)
Lifespan and skeletal muscle properties the effects of genetic background, physical activity and aging Karvinen, Sira (University of Jyväskylä, 2016)Obesity and metabolic disorders have become a notable world-wide epidemic. The pathogenesis of metabolic diseases, such as metabolic syndrome and type 2 diabetes, has begun to negatively affect life expectancy of current ...