dc.contributor.author | Chen, Jian-Hua | |
dc.contributor.author | Vanslembrouck, Bieke | |
dc.contributor.author | Ekman, Axel | |
dc.contributor.author | Aho, Vesa | |
dc.contributor.author | Larabell, Carolyn A. | |
dc.contributor.author | Le Gros, Mark A. | |
dc.contributor.author | Vihinen-Ranta, Maija | |
dc.contributor.author | Weinhardt, Venera | |
dc.date.accessioned | 2023-01-03T07:55:39Z | |
dc.date.available | 2023-01-03T07:55:39Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Chen, J.-H., Vanslembrouck, B., Ekman, A., Aho, V., Larabell, C. A., Le Gros, M. A., Vihinen-Ranta, M., & Weinhardt, V. (2022). Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells. <i>Viruses</i>, <i>14</i>(12), 2651. <a href="https://doi.org/10.3390/v14122651" target="_blank">https://doi.org/10.3390/v14122651</a> | |
dc.identifier.other | CONVID_164829491 | |
dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/84688 | |
dc.description.abstract | Upon infection, viruses hijack the cell machinery and remodel host cell structures to utilize them for viral proliferation. Since viruses are about a thousand times smaller than their host cells, imaging virus-host interactions at high spatial resolution is like looking for a needle in a haystack. Scouting gross cellular changes with fluorescent microscopy is only possible for well-established viruses, where fluorescent tagging is developed. Soft X-ray tomography (SXT) offers 3D imaging of entire cells without the need for chemical fixation or labeling. Here, we use full-rotation SXT to visualize entire human B cells infected by the herpes simplex virus 1 (HSV-1). We have mapped the temporospatial remodeling of cells during the infection and observed changes in cellular structures, such as the presence of cytoplasmic stress granules and multivesicular structures, formation of nuclear virus-induced dense bodies, and aggregates of capsids. Our results demonstrate the power of SXT imaging for scouting virus-induced changes in infected cells and understanding the orchestration of virus-host remodeling quantitatively. | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | |
dc.publisher | MDPI AG | |
dc.relation.ispartofseries | Viruses | |
dc.rights | CC BY 4.0 | |
dc.subject.other | X-ray tomography | |
dc.subject.other | soft X-rays | |
dc.subject.other | infection imaging | |
dc.subject.other | HSV-1 | |
dc.subject.other | cell mapping | |
dc.subject.other | cryo imaging | |
dc.title | Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells | |
dc.type | article | |
dc.identifier.urn | URN:NBN:fi:jyu-202301031046 | |
dc.contributor.laitos | Bio- ja ympäristötieteiden laitos | fi |
dc.contributor.laitos | Department of Biological and Environmental Science | en |
dc.contributor.oppiaine | Solu- ja molekyylibiologia | fi |
dc.contributor.oppiaine | Nanoscience Center | fi |
dc.contributor.oppiaine | Cell and Molecular Biology | en |
dc.contributor.oppiaine | Nanoscience Center | en |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.description.reviewstatus | peerReviewed | |
dc.format.pagerange | 2651 | |
dc.relation.issn | 1999-4915 | |
dc.relation.numberinseries | 12 | |
dc.relation.volume | 14 | |
dc.type.version | publishedVersion | |
dc.rights.copyright | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. | |
dc.rights.accesslevel | openAccess | fi |
dc.relation.grantnumber | 101017116 | |
dc.relation.grantnumber | 101017116 | |
dc.relation.grantnumber | 330896 | |
dc.relation.grantnumber | 6420-1252f | |
dc.relation.grantnumber | 3741-e71e8 | |
dc.relation.grantnumber | | |
dc.relation.projectid | info:eu-repo/grantAgreement/EC/H2020/101017116/EU//CoCID | |
dc.subject.yso | virukset | |
dc.subject.yso | isäntäsolut | |
dc.subject.yso | herpes simplex -virus | |
dc.subject.yso | infektiot | |
dc.subject.yso | tomografia | |
dc.subject.yso | mikroskopia | |
dc.subject.yso | solubiologia | |
dc.subject.yso | herpesvirukset | |
dc.subject.yso | solut | |
dc.subject.yso | röntgenkuvaus | |
dc.subject.yso | herpesvirusinfektiot | |
dc.format.content | fulltext | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p1123 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p27923 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p7738 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p7316 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p17798 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p16290 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p18492 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p21634 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p2409 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p10181 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p11103 | |
dc.rights.url | https://creativecommons.org/licenses/by/4.0/ | |
dc.relation.doi | 10.3390/v14122651 | |
dc.relation.funder | European Commission | en |
dc.relation.funder | Research Council of Finland | en |
dc.relation.funder | Jane and Aatos Erkko Foundation | en |
dc.relation.funder | Jane and Aatos Erkko Foundation | en |
dc.relation.funder | Jane and Aatos Erkko Foundation | en |
dc.relation.funder | Euroopan komissio | fi |
dc.relation.funder | Suomen Akatemia | fi |
dc.relation.funder | Jane ja Aatos Erkon säätiö | fi |
dc.relation.funder | Jane ja Aatos Erkon säätiö | fi |
dc.relation.funder | Jane ja Aatos Erkon säätiö | fi |
jyx.fundingprogram | RIA Research and Innovation Action, H2020 | en |
jyx.fundingprogram | Academy Project, AoF | en |
jyx.fundingprogram | Foundation | en |
jyx.fundingprogram | Foundation | en |
jyx.fundingprogram | Foundation | en |
jyx.fundingprogram | RIA Research and Innovation Action, H2020 | fi |
jyx.fundingprogram | Akatemiahanke, SA | fi |
jyx.fundingprogram | Säätiö | fi |
jyx.fundingprogram | Säätiö | fi |
jyx.fundingprogram | Säätiö | fi |
jyx.fundinginformation | The National Center for X-ray Tomography is supported by NIH NIGMS (P41GM103445, P30GM138441) and the DOE’s Office of Biological and Environmental Research (DE-AC025CH11231). V.W. was supported by the ERC Synergy Grant IndiGene (no. 810172 to Jochen Wittbrodt). M.V.-R. was supported by the Jane and Aatos Erkko Foundation and the Academy of Finland (grant number 330896). V.W. and M.V.-R. are supported by the CoCID project (no. 101017116) funded by EU Research and Innovation Act. | |
dc.type.okm | A1 | |