Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane
Aho, V., Salminen, S., Mattola, S., Gupta, A., Flomm, F., Sodeik, B., Bosse, J. B., & Vihinen-Ranta, M. (2021). Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane. PLoS Pathogens, 17(12), Article e1010132. https://doi.org/10.1371/journal.ppat.1010132
Published inPLoS Pathogens
DisciplineSolu- ja molekyylibiologiaNanotiedeNanoscience CenterCell and Molecular BiologyNanoscienceNanoscience Center
© 2021 the Authors
Herpes simplex virus capsids are assembled and packaged in the nucleus and move by diffusion through the nucleoplasm to the nuclear envelope for egress. Analyzing their motion provides conclusions not only on capsid transport but also on the properties of the nuclear environment during infection. We utilized live-cell imaging and single-particle tracking to characterize capsid motion relative to the host chromatin. The data indicate that as the chromatin was marginalized toward the nuclear envelope it presented a restrictive barrier to the capsids. However, later in infection this barrier became more permissive and the probability of capsids to enter the chromatin increased. Thus, although chromatin marginalization initially restricted capsid transport to the nuclear envelope, a structural reorganization of the chromatin counteracted that to promote capsid transport later. Analyses of capsid motion revealed that it was subdiffusive, and that the diffusion coefficients were lower in the chromatin than in regions lacking chromatin. In addition, the diffusion coefficient in both regions increased during infection. Throughout the infection, the capsids were never enriched at the nuclear envelope, which suggests that instead of nuclear export the transport through the chromatin is the rate-limiting step for the nuclear egress of capsids. This provides motivation for further studies by validating the importance of intranuclear transport to the life cycle of HSV-1. ...
PublisherPublic Library of Science (PLoS)
Publication in research information system
MetadataShow full item record
Related funder(s)Academy of Finland; European Commission
Funding program(s)Academy Project, AoF
The content of the publication reflects only the author’s view. The funder is not responsible for any use that may be made of the information it contains.
Additional information about fundingThis work was financed by the Jane and Aatos Erkko Foundation (MVR); Academy of Finland under the award number 330896 (MVR); European Union’s Horizon 2020 research and innovation programme under grant agreement No 101017116, project CoCID (Compact Cell-Imaging Device, MVR); the Graduate School of the University of Jyvaskyla (SM). JBB is funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2155 – project number 390874280. ...
Showing items with similar title or keywords.
Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection Aho, Vesa; Mäntylä, Elina; Ekman, Axel; Hakanen, Satu; Mattola, Salla; Chen, Jian-Hua; Weinhardt, Venera; Ruokolainen, Visa; Sodeik, Beate; Larabell, Carolyn; Vihinen-Ranta, Maija (MDPI, 2019)During lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 ...
Salminen, Sami (2019)Herpes simplex virus 1 (HSV-1) is a human pathogenic dsDNA virus capable of lytic orolabial infections in humans. Initial lytic infections lead to life-long and sporadically reactivating latent infections. In a lytic ...
The Minor Capsid Protein VP11 of Thermophilic Bacteriophage P23-77 Facilitates Virus Assembly by Using Lipid-Protein Interactions Pawlowski, Alice; Moilanen, Anni M.; Rissanen, Ilona; Määttä, Juha A. E.; Hytönen, Vesa P.; Ihalainen, Janne; Bamford, Jaana (American Society for Microbiology, 2015)Thermus thermophilus bacteriophage P23-77 is the type member of a new virus family of icosahedral, tailless, inner-membranecontaining double-stranded DNA (dsDNA) viruses infecting thermophilic bacteria and halophilic ...
Mäntylä, Elina (University of Jyväskylä, 2016)
Garver, Kyle A.; Leskisenoja, Katja; Macrae, Robert; Hawley, Laura M.; Subramaniam, Kuttichantran; Waltzek, Thomas B.; Richard, Jon; Josefsson, Caroline; Valtonen, Tellervo (Inter-Research, 2018)The order Herpesvirales includes viruses that infect aquatic and terrestrial vertebrates and several aquatic invertebrates (i.e. mollusks), and share the commonality of possessing a double-stranded DNA core surrounded ...