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dc.contributor.authorde Freitas Almeida, Gabriel Magno
dc.contributor.authorHoikkala, Ville
dc.contributor.authorRavantti, Janne
dc.contributor.authorRantanen, Noora
dc.contributor.authorSundberg, Lotta-Riina
dc.date.accessioned2022-08-18T07:47:02Z
dc.date.available2022-08-18T07:47:02Z
dc.date.issued2022
dc.identifier.citationde Freitas Almeida, G. M., Hoikkala, V., Ravantti, J., Rantanen, N., & Sundberg, L.-R. (2022). Mucin induces CRISPR-Cas defense in an opportunistic pathogen. <i>Nature Communications</i>, <i>13</i>, Article 3653. <a href="https://doi.org/10.1038/s41467-022-31330-3" target="_blank">https://doi.org/10.1038/s41467-022-31330-3</a>
dc.identifier.otherCONVID_147396498
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/82685
dc.description.abstractParasitism by bacteriophages has led to the evolution of a variety of defense mechanisms in their host bacteria. However, it is unclear what factors lead to specific defenses being deployed upon phage infection. To explore this question, we co-evolved the bacterial fish pathogen Flavobacterium columnare and its virulent phage V156 in presence and absence of a eukaryotic host signal (mucin) for sixteen weeks. The presence of mucin leads to a dramatic increase in CRISPR spacer acquisition, especially in low nutrient conditions where over 60% of colonies obtain at least one new spacer. Additionally, we show that the presence of a competitor bacterium further increases CRISPR spacer acquisition in F. columnare. These results suggest that ecological factors are important in determining defense strategies against phages, and that the phage-bacterium interactions on mucosal surfaces may select for the diversification of bacterial immune systems.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofseriesNature Communications
dc.rightsCC BY 4.0
dc.subject.otherbacteriophages
dc.subject.otherCRISPR-Cas systems
dc.subject.othermicrobial ecology
dc.titleMucin induces CRISPR-Cas defense in an opportunistic pathogen
dc.typeresearch article
dc.identifier.urnURN:NBN:fi:jyu-202208184225
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineResurssiviisausyhteisöfi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineSchool of Resource Wisdomen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn2041-1723
dc.relation.volume13
dc.type.versionpublishedVersion
dc.rights.copyright© The Author(s) 2022
dc.rights.accesslevelopenAccessfi
dc.type.publicationarticle
dc.relation.grantnumber314939
dc.relation.grantnumber
dc.subject.ysomikrobiekologia
dc.subject.ysobakteerit
dc.subject.ysoparasitismi
dc.subject.ysoimmuunijärjestelmä
dc.subject.ysopuolustusmekanismit (biologia)
dc.subject.ysobakteriofagit
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p16314
jyx.subject.urihttp://www.yso.fi/onto/yso/p1749
jyx.subject.urihttp://www.yso.fi/onto/yso/p8362
jyx.subject.urihttp://www.yso.fi/onto/yso/p16041
jyx.subject.urihttp://www.yso.fi/onto/yso/p6078
jyx.subject.urihttp://www.yso.fi/onto/yso/p25303
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1038/s41467-022-31330-3
dc.relation.funderResearch Council of Finlanden
dc.relation.funderEmil Aaltonen Foundationen
dc.relation.funderSuomen Akatemiafi
dc.relation.funderEmil Aaltosen Säätiö srfi
jyx.fundingprogramAcademy Project, AoFen
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundinginformationThis study was funded by the Academy of Finland grant #314939 (L.-R.S.), and by Emil Aaltonen Foundation grant (L.-R.S.)
datacite.isSupplementedBy.doi10.17011/jyx/dataset/81288
datacite.isSupplementedByHoikkala, Ville; Rantanen, Noora; Sundberg, Lotta-Riina; Ravantti, Janne; Almeida, Gabriel MF. (2022). <i>Dataset for Mucin induces CRISPR-Cas defense in an opportunistic pathogen</i>. V. 24.5.2022. University of Jyväskylä. <a href="https://doi.org/10.17011/jyx/dataset/81288" target="_blank">https://doi.org/10.17011/jyx/dataset/81288</a>. <a href="http://urn.fi/URN:NBN:fi:jyu-202205252912">https://urn.fi/URN:NBN:fi:jyu-202205252912</a>
dc.type.okmA1


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