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dc.contributor.authorLehtivuori, Heli
dc.contributor.authorRumfeldt, Jessica
dc.contributor.authorMustalahti, Satu
dc.contributor.authorKurkinen, Sami
dc.contributor.authorTakala, Heikki
dc.date.accessioned2022-08-12T07:04:34Z
dc.date.available2022-08-12T07:04:34Z
dc.date.issued2022
dc.identifier.citationLehtivuori, H., Rumfeldt, J., Mustalahti, S., Kurkinen, S., & Takala, H. (2022). Conserved histidine and tyrosine determine spectral responses through the water network in Deinococcus radiodurans phytochrome. <i>Photochemical and Photobiological Sciences</i>, <i>21</i>(11), 1975-1989. <a href="https://doi.org/10.1007/s43630-022-00272-6" target="_blank">https://doi.org/10.1007/s43630-022-00272-6</a>
dc.identifier.otherCONVID_150995821
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/82490
dc.description.abstractPhytochromes are red light-sensing photoreceptor proteins that bind a bilin chromophore. Here, we investigate the role of a conserved histidine (H260) and tyrosine (Y263) in the chromophore-binding domain (CBD) of Deinococcus radiodurans phytochrome (DrBphP). Using crystallography, we show that in the H260A variant, the missing imidazole side chain leads to increased water content in the binding pocket. On the other hand, Y263F mutation reduces the water occupancy around the chromophore. Together, these changes in water coordination alter the protonation and spectroscopic properties of the biliverdin. These results pinpoint the importance of this conserved histidine and tyrosine, and the related water network, for the function and applications of phytochromes.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherSpringer Science and Business Media LLC
dc.relation.ispartofseriesPhotochemical and Photobiological Sciences
dc.rightsCC BY 4.0
dc.subject.otherfytokromit
dc.subject.otherspectral responses
dc.subject.otherwater network
dc.subject.otherbiliverdin protonation
dc.subject.otherphytochrome structure
dc.titleConserved histidine and tyrosine determine spectral responses through the water network in Deinococcus radiodurans phytochrome
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202208124034
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosKemian laitosfi
dc.contributor.laitosFysiikan laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.laitosDepartment of Chemistryen
dc.contributor.laitosDepartment of Physicsen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineFysikaalinen kemiafi
dc.contributor.oppiaineFysiikkafi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineNanoscience Centeren
dc.contributor.oppiainePhysical Chemistryen
dc.contributor.oppiainePhysicsen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.format.pagerange1975-1989
dc.relation.issn1474-905X
dc.relation.numberinseries11
dc.relation.volume21
dc.type.versionpublishedVersion
dc.rights.copyright© The Author(s) 2022
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber290677
dc.relation.grantnumber330678
dc.relation.grantnumber277194
dc.subject.ysovalo
dc.subject.ysoproteiinit
dc.subject.ysovalokemia
dc.subject.ysosäteily
dc.subject.ysobakteerit
dc.subject.ysokidetiede
dc.subject.ysofotobiologia
dc.subject.ysospektroskopia
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p5742
jyx.subject.urihttp://www.yso.fi/onto/yso/p4332
jyx.subject.urihttp://www.yso.fi/onto/yso/p7201
jyx.subject.urihttp://www.yso.fi/onto/yso/p4150
jyx.subject.urihttp://www.yso.fi/onto/yso/p1749
jyx.subject.urihttp://www.yso.fi/onto/yso/p643
jyx.subject.urihttp://www.yso.fi/onto/yso/p27666
jyx.subject.urihttp://www.yso.fi/onto/yso/p10176
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1007/s43630-022-00272-6
dc.relation.funderResearch Council of Finlanden
dc.relation.funderResearch Council of Finlanden
dc.relation.funderResearch Council of Finlanden
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
jyx.fundingprogramAcademy Project, AoFen
jyx.fundingprogramAcademy Research Fellow, AoFen
jyx.fundingprogramPostdoctoral Researcher, AoFen
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundingprogramAkatemiatutkija, SAfi
jyx.fundingprogramTutkijatohtori, SAfi
jyx.fundinginformationThis work was supported by Academy of Finland grants 285461 (H.T.), 330678 (H.T., J.R.), 277194 (H.L.), and 290677 (S.M.).
dc.type.okmA1


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