Modulation of Structural Heterogeneity Controls Phytochrome Photoswitching
Gustavsson, E., Isaksson, L., Persson, C., Mayzel, M., Brath, U., Vrhovac, L., Ihalainen, J. A., Karlsson, B. G., Orekhov, V., & Westenhoff, S. (2020). Modulation of Structural Heterogeneity Controls Phytochrome Photoswitching. Biophysical Journal, 118(2), 415-421. https://doi.org/10.1016/j.bpj.2019.11.025
Published inBiophysical Journal
© 2019 Biophysical Society
Phytochromes sense red/far-red light and control many biological processes in plants, fungi, and bacteria. Although crystal structures of dark and light adapted states have been determined, the molecular mechanisms underlying photoactivation remains elusive. Here we demonstrate that the conserved tongue region of the PHY domain of a 57kDa photosensory module of Deinococcus radiodurans phytochrome, changes from a structurally heterogeneous dark state to an ordered light activated state. The results were obtained in solution by utilizing a laser-triggered activation approach detected on the atomic level with high-resolution protein NMR spectroscopy. The data suggest that photosignaling of phytochromes relies on careful modulation of structural heterogeneity of the PHY tongue.
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Related funder(s)Academy of Finland
Funding program(s)Academy Project, AoF
Additional information about fundingSebastian Westenhoff thanks the Knut and Alice Wallenberg Foundation for an Academy Fellowship. Janne Ihalainen acknowledges the Academy of Finland grant 296135 and Jane and Aatos Erkko foundation. Vladislav Orekhov thanks the support by the Swedish Research Council (Research Grant 201504614). NMR spectroscopy was carried out at the Swedish NMR Centre at University of Gothenburg, supported by the Knut and Alice Wallenberg Foundation (NMR for Life) and the Science for Life Laboratory (SciLifeLab). ...
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