Hydrogen isotopes (δ2H) of polyunsaturated fatty acids track bioconversion by zooplankton
Pilecky, M., Kämmer, S. K., Mathieu‐Resuge, M., Wassenaar, L. I., Taipale, S. J., Martin‐Creuzburg, D., & Kainz, M. J. (2022). Hydrogen isotopes (δ2H) of polyunsaturated fatty acids track bioconversion by zooplankton. Functional Ecology, 36(3), 538-549. https://doi.org/10.1111/1365-2435.13981
Published inFunctional Ecology
© 2021 The Authors. Functional Ecology published by John Wiley & Sons Ltd on behalf of British Ecological Society.
1. Organisms at the base of aquatic food webs synthesize essential nutrients, such as omega-3 polyunsaturated fatty acids (n-3 PUFA), which are transferred to consumers at higher trophic levels. Many consumers, requiring n-3 long-chain (LC) PUFA, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have limited ability to biosynthesize them from the essential dietary precursor α-linolenic acid (ALA) and thus rely on dietary provision of LC-PUFA. 2. We investigated LC-PUFA metabolism in freshwater zooplankton using stable hydrogen isotopes (δ2H) of fatty acids as tracers. We conducted feeding experiments with the freshwater keystone grazer Daphnia to quantify changes in the δ2H value of body FA in response to the FA composition of their food and the δ2H value of the ambient water. 3. The isotopic composition of LC-PUFA changed in Daphnia, depending on the integration of 2H from ambient water during de novo synthesis or bioconversion from dietary precursors, allowing us to distinguish dietary from bioconverted EPA in body tissue. We tested the applicability of these laboratory findings in a field setting by analyzing δ2H values of PUFA in primary producers and consumers in eutrophic ponds to track EPA sources of zooplankton. 4. Multilinear regression models that included conversion of ALA to EPA correlated better with zooplankton δ2HEPA than seston δ2HEPA at low dietary EPA supply. 5. This study provides evidence that zooplankton can compensate for low dietary EPA supply by activating LC-PUFA biosynthesis and shows that herbivorous zooplankton play a crucial role in upgrading FA for higher trophic levels during low dietary EPA supply. ...
Publication in research information system
MetadataShow full item record
Additional information about fundingThis work has been supported by the Austrian Science Fund (FWF; I-3855) and the German Research Foundation (DFG; MA 5005/8-1) within the framework of the DACH collaboration (project ‘AquaTerr’).
Showing items with similar title or keywords.
Poor nutritional quality of primary producers and zooplankton driven by eutrophication is mitigated at upper trophic levels Taipale, Sami Johan; Ventelä, Anne‐Mari; Litmanen, Jaakko; Anttila, Lauri (John Wiley & Sons, 2022)Eutrophication and rising water temperature in freshwaters may increase the total production of a lake while simultaneously reducing the nutritional quality of food web components. We evaluated how cyanobacteria blooms, ...
Lowered nutritional quality of prey decrease the growth and biomolecule content of rainbow trout fry Taipale, Sami J.; Pulkkinen, Katja; Keva, Ossi; Kainz, Martin J.; Nykänen, Hannu (Elsevier Inc., 2022)Diet quality is crucial for the development of offspring. Here, we examined how the nutritional quality of prey affects somatic growth and the lipid, carbohydrate, protein, amino acid, and polyunsaturated fatty acid content ...
Taipale, Sami J.; Kuoppamäki, K.; Strandberg, U.; Peltomaa, E.; Vuorio, K. (Springer, 2020)Food quality is one of the key factors influencing zooplankton population dynamics. Eutrophication drives phytoplankton communities toward the dominance of cyanobacteria, which means a decrease in the availability of sterols ...
Interacting effects of simulated eutrophication, temperature increase, and microplastic exposure on Daphnia Hiltunen, Minna; Vehniäinen, Eeva-Riikka; Kukkonen, Jussi V. K. (Elsevier BV, 2021)The effects of multiple stressors are difficult to separate in field studies, and their interactions may be hard to predict if studied in isolation. We studied the effects of decreasing food quality (increase in cyanobacteria ...
Peltomaa, Elina T.; Aalto, Sanni L.; Vuorio, Kristiina M.; Taipale, Sami (Frontiers Media S.A., 2017)The growth and reproduction of animals is affected by their access to resources. In aquatic ecosystems, the availability of essential biomolecules for filter-feeding zooplankton depends greatly on phytoplankton. Here, we ...