Crowdsourced analysis of fungal growth and branching on microfluidic platforms
Hopke, A., Mela, A., Ellett, F., Carter-House, D., Peña, J. F., Stajich, J. E., Altamirano, S., Lovett, B., Egan, M., Kale, S., Kronholm, I., Guerette, P., Szewczyk, E., McCluskey, K., Breslauer, D., Shah, H., Coad, B. R., Momany, M., & Irimia, D. (2021). Crowdsourced analysis of fungal growth and branching on microfluidic platforms. PLoS ONE, 16(9), Article e0257823. https://doi.org/10.1371/journal.pone.0257823
Published inPLoS ONE
DisciplineBiologisten vuorovaikutusten huippututkimusyksikköEkologia ja evoluutiobiologiaCentre of Excellence in Biological Interactions ResearchEcology and Evolutionary Biology
© 2021 the Authors
Fungal hyphal growth and branching are essential traits that allow fungi to spread and proliferate in many environments. This sustained growth is essential for a myriad of applications in health, agriculture, and industry. However, comparisons between different fungi are difficult in the absence of standardized metrics. Here, we used a microfluidic device featuring four different maze patterns to compare the growth velocity and branching frequency of fourteen filamentous fungi. These measurements result from the collective work of several labs in the form of a competition named the “Fungus Olympics.” The competing fungi included five ascomycete species (ten strains total), two basidiomycete species, and two zygomycete species. We found that growth velocity within a straight channel varied from 1 to 4 μm/min. We also found that the time to complete mazes when fungal hyphae branched or turned at various angles did not correlate with linear growth velocity. We discovered that fungi in our study used one of two distinct strategies to traverse mazes: high-frequency branching in which all possible paths were explored, and low-frequency branching in which only one or two paths were explored. While the high-frequency branching helped fungi escape mazes with sharp turns faster, the low-frequency turning had a significant advantage in mazes with shallower turns. Future work will more systematically examine these trends. ...
PublisherPublic Library of Science (PLoS)
Publication in research information system
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Additional information about fundingFunding to the Irimia lab included support from NIH GM092804 and EB002503. Funding to the Momany lab included support from the Burroughs Wellcome Fund CRT1017499. Funding to the Stajich lab included support from NSF DEB-1441715. Stajich is a CIFAR fellow in the program Fungal Kingdom: Threats and Opportunities.
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