dc.contributor.author | Civil, Rita | |
dc.contributor.author | Brook, Matthew S. | |
dc.contributor.author | Elliott‐Sale, Kirsty J. | |
dc.contributor.author | Santos, Lívia | |
dc.contributor.author | Varley, Ian | |
dc.contributor.author | Lensu, Sanna | |
dc.contributor.author | Kainulainen, Heikki | |
dc.contributor.author | Koch, Lauren G. | |
dc.contributor.author | Britton, Steven L. | |
dc.contributor.author | Wilkinson, Daniel J. | |
dc.contributor.author | Smith, Kenneth | |
dc.contributor.author | Sale, Craig | |
dc.contributor.author | Atherton, Philip J. | |
dc.date.accessioned | 2021-05-31T10:43:44Z | |
dc.date.available | 2021-05-31T10:43:44Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Civil, R., Brook, M. S., Elliott‐Sale, K. J., Santos, L., Varley, I., Lensu, S., Kainulainen, H., Koch, L. G., Britton, S. L., Wilkinson, D. J., Smith, K., Sale, C., & Atherton, P. J. (2021). A collagen extraction and deuterium oxide stable isotope tracer method for the quantification of bone collagen synthesis rates in vivo. <i>Physiological Reports</i>, <i>9</i>(10), Article e14799. <a href="https://doi.org/10.14814/phy2.14799" target="_blank">https://doi.org/10.14814/phy2.14799</a> | |
dc.identifier.other | CONVID_89738048 | |
dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/76082 | |
dc.description.abstract | The development of safe and practical strategies to prevent weakening of bone tissue is vital, yet attempts to achieve this have been hindered by a lack of understanding of the short-term (days-weeks) physiology of bone collagen turnover. To address this, we have developed a method to quantify bone collagen synthesis in vivo, using deuterium oxide (D2O) tracer incorporation techniques combined with gas chromatography pyrolysis isotope-ratio mass spectrometry (GC-pyrolysis-IRMS). Forty-six male and female rats from a selectively bred model ingested D2O for 3 weeks. Femur diaphyses (FEM), tibia proximal (T-PRO), and distal (T-DIS) epiphyses-metaphyses and tibia mid-shaft diaphyses (T-MID) were obtained from all rats after necropsy. After demineralisation, collagen proteins were isolated and hydrolysed and collagen fractional synthetic rates (FSRs) determined by incorporation of deuterium into protein-bound alanine via GC-pyrolysis-IRMS. The collagen FSR for the FEM (0.131 ± 0.078%/day; 95% CI [0.106–0.156]) was greater than the FSR at T-MID (0.055 ± 0.049%/day; 95% CI [0.040–0.070]; p < 0.001). The T-PRO site had the highest FSR (0.203 ± 0.123%/day; 95% CI [0.166–0.241]) and T-DIS the lowest (0.027 ± 0.015%/day; 95% CI [0.022–0.031]). The three tibial sites exhibited different FSRs (p < 0.001). Herein, we have developed a sensitive method to quantify in vivo bone collagen synthesis and identified site-specific rates of synthesis, which could be applicable to studies of human bone collagen turnover. | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | |
dc.publisher | John Wiley & Sons | |
dc.relation.ispartofseries | Physiological Reports | |
dc.rights | CC BY 4.0 | |
dc.subject.other | bone turnover | |
dc.subject.other | collagen synthesis | |
dc.subject.other | deuterium oxide | |
dc.subject.other | GC-pyrolysis-IRMS | |
dc.subject.other | stable isotopes | |
dc.title | A collagen extraction and deuterium oxide stable isotope tracer method for the quantification of bone collagen synthesis rates in vivo | |
dc.type | article | |
dc.identifier.urn | URN:NBN:fi:jyu-202105313325 | |
dc.contributor.laitos | Liikuntatieteellinen tiedekunta | fi |
dc.contributor.laitos | Faculty of Sport and Health Sciences | en |
dc.contributor.oppiaine | Liikuntalääketiede | fi |
dc.contributor.oppiaine | Liikuntafysiologia | fi |
dc.contributor.oppiaine | Sports and Exercise Medicine | en |
dc.contributor.oppiaine | Exercise Physiology | en |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.description.reviewstatus | peerReviewed | |
dc.relation.issn | 2051-817X | |
dc.relation.numberinseries | 10 | |
dc.relation.volume | 9 | |
dc.type.version | publishedVersion | |
dc.rights.copyright | © 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society | |
dc.rights.accesslevel | openAccess | fi |
dc.subject.yso | luu | |
dc.subject.yso | biomarkkerit | |
dc.subject.yso | regeneraatio (biologia) | |
dc.subject.yso | isotooppianalyysi | |
dc.subject.yso | kollageenit | |
dc.subject.yso | luukudokset | |
dc.format.content | fulltext | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p24244 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p12288 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p17818 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p38901 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p8590 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p24381 | |
dc.rights.url | https://creativecommons.org/licenses/by/4.0/ | |
dc.relation.doi | 10.14814/phy2.14799 | |
jyx.fundinginformation | Versus Arthritis; Medical Research Council | |
dc.type.okm | A1 | |