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dc.contributor.authorCivil, Rita
dc.contributor.authorBrook, Matthew S.
dc.contributor.authorElliott‐Sale, Kirsty J.
dc.contributor.authorSantos, Lívia
dc.contributor.authorVarley, Ian
dc.contributor.authorLensu, Sanna
dc.contributor.authorKainulainen, Heikki
dc.contributor.authorKoch, Lauren G.
dc.contributor.authorBritton, Steven L.
dc.contributor.authorWilkinson, Daniel J.
dc.contributor.authorSmith, Kenneth
dc.contributor.authorSale, Craig
dc.contributor.authorAtherton, Philip J.
dc.date.accessioned2021-05-31T10:43:44Z
dc.date.available2021-05-31T10:43:44Z
dc.date.issued2021
dc.identifier.citationCivil, R., Brook, M. S., Elliott‐Sale, K. J., Santos, L., Varley, I., Lensu, S., Kainulainen, H., Koch, L. G., Britton, S. L., Wilkinson, D. J., Smith, K., Sale, C., & Atherton, P. J. (2021). A collagen extraction and deuterium oxide stable isotope tracer method for the quantification of bone collagen synthesis rates in vivo. <i>Physiological Reports</i>, <i>9</i>(10), Article e14799. <a href="https://doi.org/10.14814/phy2.14799" target="_blank">https://doi.org/10.14814/phy2.14799</a>
dc.identifier.otherCONVID_89738048
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/76082
dc.description.abstractThe development of safe and practical strategies to prevent weakening of bone tissue is vital, yet attempts to achieve this have been hindered by a lack of understanding of the short-term (days-weeks) physiology of bone collagen turnover. To address this, we have developed a method to quantify bone collagen synthesis in vivo, using deuterium oxide (D2O) tracer incorporation techniques combined with gas chromatography pyrolysis isotope-ratio mass spectrometry (GC-pyrolysis-IRMS). Forty-six male and female rats from a selectively bred model ingested D2O for 3 weeks. Femur diaphyses (FEM), tibia proximal (T-PRO), and distal (T-DIS) epiphyses-metaphyses and tibia mid-shaft diaphyses (T-MID) were obtained from all rats after necropsy. After demineralisation, collagen proteins were isolated and hydrolysed and collagen fractional synthetic rates (FSRs) determined by incorporation of deuterium into protein-bound alanine via GC-pyrolysis-IRMS. The collagen FSR for the FEM (0.131 ± 0.078%/day; 95% CI [0.106–0.156]) was greater than the FSR at T-MID (0.055 ± 0.049%/day; 95% CI [0.040–0.070]; p < 0.001). The T-PRO site had the highest FSR (0.203 ± 0.123%/day; 95% CI [0.166–0.241]) and T-DIS the lowest (0.027 ± 0.015%/day; 95% CI [0.022–0.031]). The three tibial sites exhibited different FSRs (p < 0.001). Herein, we have developed a sensitive method to quantify in vivo bone collagen synthesis and identified site-specific rates of synthesis, which could be applicable to studies of human bone collagen turnover.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherJohn Wiley & Sons
dc.relation.ispartofseriesPhysiological Reports
dc.rightsCC BY 4.0
dc.subject.otherbone turnover
dc.subject.othercollagen synthesis
dc.subject.otherdeuterium oxide
dc.subject.otherGC-pyrolysis-IRMS
dc.subject.otherstable isotopes
dc.titleA collagen extraction and deuterium oxide stable isotope tracer method for the quantification of bone collagen synthesis rates in vivo
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202105313325
dc.contributor.laitosLiikuntatieteellinen tiedekuntafi
dc.contributor.laitosFaculty of Sport and Health Sciencesen
dc.contributor.oppiaineLiikuntalääketiedefi
dc.contributor.oppiaineLiikuntafysiologiafi
dc.contributor.oppiaineSports and Exercise Medicineen
dc.contributor.oppiaineExercise Physiologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn2051-817X
dc.relation.numberinseries10
dc.relation.volume9
dc.type.versionpublishedVersion
dc.rights.copyright© 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society
dc.rights.accesslevelopenAccessfi
dc.subject.ysoluu
dc.subject.ysobiomarkkerit
dc.subject.ysoregeneraatio (biologia)
dc.subject.ysoisotooppianalyysi
dc.subject.ysokollageenit
dc.subject.ysoluukudokset
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p24244
jyx.subject.urihttp://www.yso.fi/onto/yso/p12288
jyx.subject.urihttp://www.yso.fi/onto/yso/p17818
jyx.subject.urihttp://www.yso.fi/onto/yso/p38901
jyx.subject.urihttp://www.yso.fi/onto/yso/p8590
jyx.subject.urihttp://www.yso.fi/onto/yso/p24381
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.14814/phy2.14799
jyx.fundinginformationVersus Arthritis; Medical Research Council
dc.type.okmA1


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