The effects of endurance trainability phenotype, sex, and interval running training on bone collagen synthesis in adult rats
Civil, R., Brook, M. S., Santos, L., Varley, I., Elliott-Sale, K. J., Lensu, S., Ahtiainen, J. P., Kainulainen, H., Koch, L. G., Britton, S. L., Wilkinson, D. J., Smith, K., Atherton, P. J., & Sale, C. (2024). The effects of endurance trainability phenotype, sex, and interval running training on bone collagen synthesis in adult rats. Bone, 189, Article 117257. https://doi.org/10.1016/j.bone.2024.117257
Published in
BoneAuthors
Date
2024Copyright
© 2024 The Authors. Published by Elsevier Inc.
Bone is influenced by many factors such as genetics and mechanical loading, but the short-term physiological effects of these factors on bone (re)modelling are not well characterised. This study investigated the effects of endurance trainability phenotype, sex, and interval running training (7-week intervention) on bone collagen formation in rats using a deuterium oxide stable isotope tracer method. Bone samples of the femur diaphysis, proximal tibia, mid-shaft tibia, and distal tibia were collected after necropsy from forty-six 9 ± 3-month male and female rats selectively bred for yielding low (LRT) or high (HRT) responses to endurance training. Bone collagen proteins were isolated and hydrolysed, and fractional synthetic rates (FSRs) were determined by the incorporation of deuterium into protein-bound alanine via GC-pyrolysis-IRMS. There was a significant large main effect of phenotype at the femur site (p < 0.001; η2g = 0.473) with HRT rats showing greater bone collagen FSRs than LRT rats. There was a significant large main effect of phenotype (p = 0.008; η2g = 0.178) and a significant large main effect of sex (p = 0.005; η2g = 0.196) at the proximal site of the tibia with HRT rats showing greater bone collagen FSRs than LRT rats, and male rats showing greater bone collagen FSRs compared to female rats. There was a significant large main effect of training at the mid-shaft site of the tibia (p = 0.012; η2g = 0.159), with rats that underwent interval running training having greater bone collagen FSRs than control rats. Similarly, there was a significant large main effect of training at the distal site of the tibia (p = 0.050; η2g = 0.156), with rats in the interval running training group having greater bone collagen FSRs compared to rats in the control group. Collectively, this evidence highlights that bone responses to physiological effects are site-specific, indicating that interval running training has positive effects on bone collagen synthesis at the tibial mid-shaft and distal sites, whilst genetic factors affect bone collagen synthesis at the femur diaphysis (phenotype) and proximal tibia (phenotype and sex) in rats.
...
Publisher
ElsevierISSN Search the Publication Forum
8756-3282Keywords
Publication in research information system
https://converis.jyu.fi/converis/portal/detail/Publication/243148853
Metadata
Show full item recordCollections
Related funder(s)
Research Council of FinlandFunding program(s)
Academy Project, AoFAdditional information about funding
This work was completed as part of the PhD programme of work for RC, for which she received funding from the Nottingham Trent University Vice Chancellors Studentship Scheme. SL has received funding from the Academy of Finland, and currently the Research Council of Finland (decisions 321522 and 355392). Animal samples used in this analysis were a gift from the University of Michigan. This work was also supported by the UK MRC (grant no. MR/P021220/1) as part of the MRC-ARUK Centre for Musculoskeletal Ageing Research awarded to the Universities of Nottingham and Birmingham and supported by the National Institute of Health Research (NIHR) Nottingham Biomedical Research Centre. ...License
Related items
Showing items with similar title or keywords.
-
A collagen extraction and deuterium oxide stable isotope tracer method for the quantification of bone collagen synthesis rates in vivo
Civil, Rita; Brook, Matthew S.; Elliott‐Sale, Kirsty J.; Santos, Lívia; Varley, Ian; Lensu, Sanna; Kainulainen, Heikki; Koch, Lauren G.; Britton, Steven L.; Wilkinson, Daniel J.; Smith, Kenneth; Sale, Craig; Atherton, Philip J. (John Wiley & Sons, 2021)The development of safe and practical strategies to prevent weakening of bone tissue is vital, yet attempts to achieve this have been hindered by a lack of understanding of the short-term (days-weeks) physiology of bone ... -
Variants associated with HHIP expression have sex-differential effects on lung function [version 1; peer review: 2 approved]
Fawcett, KA; Obeidat, M; Melbourne, C; Shrine, N; Guyatt, AL; John, C; Luan, J; Richmond, A; Moksnes, MR; Granell, R; Weiss, S; Imboden, M; May-Wilson, S; Hysi, P; Boutin, TS; Portas, L; Flexeder, C; Harris, SE; Wang, CA; Lyytikäinen, LP; Palviainen, T; Foong, RE; Keidel, D; Minelli, C; Langenberg, C; Bossé, Y; Van den Berge, M; Sin, DD; Hao, K; Campbell, A; Porteous, D; Padmanabhan, S; Smith, BH; Evans, DM; Ring, S; Langhammer, A; Hveem, K; Willer, C; Ewert, R; Stubbe, B; Pirastu, N; Klaric, L; Joshi, PK; Patasova, K; Massimo, M; Polasek, O; Starr, JM; Karrasch, S; Strauch, K; Meitinger, T; Rudan, I; Rantanen, T.; Pietiläinen, K; Kähönen, M; Raitakari, OT; Hall, GL; Sly, PD; Pennell, CE; Kaprio, J; Lehtimäki, T; Vitart, V; Deary, IJ; Jarvis, D; Wilson, JF; Spector, T; Probst-Hensch, N; Wareham, NJ; Völzke, H; Henderson, J; Strachan, DP; Brumpton, BM; Hayward, C; Hall, IP; Tobin, MD; Wain, LV (Wellcome Trust, 2020)Background: Lung function is highly heritable and differs between the sexes throughout life. However, little is known about sex-differential genetic effects on lung function. We aimed to conduct the first genome-wide ... -
Effects of high-intensity interval training on VO2max and post-exercise fat consumption in recreationally active adults compared to steady-state running
Stenman, Mari (2016)Johdanto. Korkeatehoista intervalliharjoittelua on käytetty jo pitkään urheilijoiden valmennuksessa. Lisäksi viime vuosikymmeninä on julkaistu useita tutkimuksia harjoitusmuodon vaikutuksesta sekä vähän liikkuvien että ... -
The effect of high-intensity interval exercise program on blood lipids and hormones in recreationally active adults
Malmivaara, Susanna (2015)Johdanto. Tyypin 2 diabetes sekä verenkieroelimistön sairaudet ovat yleistyneet ja niiden tehokkaimpana ehkäisynä pidetään liikuntaa. Suomessa ihmiset eivät noudata liikuntasuosi-tuksia ja syyksi inaktiivisuuteen sanotaankin ... -
Genetic and Environmental Effects on the Individual Variation and Continuity of Participation in Diverse Physical Activities
Kaartinen, Sara; Silventoinen, Karri; Korhonen, Tellervo; Kujala, Urho M.; Kaprio, Jaakko; Aaltonen, Sari (Lippincott Williams & Wilkins, 2021)Introduction Participation in diverse physical activities has beneficial health effects. However, little is known on how genetic and environmental factors affect this trait. Thus, we examined to what extent these factors ...