Näytä suppeat kuvailutiedot

dc.contributor.authorLaanto, Elina
dc.contributor.authorRavantti, Janne J.
dc.contributor.authorSundberg, Lotta-Riina
dc.date.accessioned2020-12-08T08:15:02Z
dc.date.available2020-12-08T08:15:02Z
dc.date.issued2020
dc.identifier.citationLaanto, E., Ravantti, J. J., & Sundberg, L.-R. (2020). Prophages and Past Prophage-Host Interactions Revealed by CRISPR Spacer Content in a Fish Pathogen. <i>Microorganisms</i>, <i>8</i>(12), Article 1919. <a href="https://doi.org/10.3390/microorganisms8121919" target="_blank">https://doi.org/10.3390/microorganisms8121919</a>
dc.identifier.otherCONVID_47278292
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/73018
dc.description.abstractThe role of prophages in the evolution, diversification, or virulence of the fish pathogen Flavobacterium columnare has not been studied thus far. Here, we describe a functional spontaneously inducing prophage fF4 from the F. columnare type strain ATCC 23463, which is not detectable with commonly used prophage search methods. We show that this prophage type has a global distribution and is present in strains isolated from Finland, Thailand, Japan, and North America. The virions of fF4 are myoviruses with contractile tails and infect only bacterial strains originating from Northern Finland. The fF4 resembles transposable phages by similar genome organization and several gene orthologs. Additional bioinformatic analyses reveal several species in the phylum Bacteroidetes that host a similar type of putative prophage, including bacteria that are important animal and human pathogens. Furthermore, a survey of F. columnare Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) spacers indicate a shared evolutionary history between F. columnare strains and the fF4 phage, and another putative prophage in the F. columnare strain ATCC 49512, named p49512. First, CRISPR spacer content from the two CRISPR loci (types II-C and VI-B) of the fF4 lysogen F. columnare ATCC 23463 revealed a phage terminase protein-matching spacer in the VI-B locus. This spacer is also present in two Chinese F. columnare strains. Second, CRISPR analysis revealed four F. columnare strains that contain unique spacers targeting different regions of the putative prophage p49512 in the F. columnare strain ATCC 49512, despite the geographical distance or genomovar of the different strains. This suggests a common ancestry for the F. columnare prophages and different host strains.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofseriesMicroorganisms
dc.rightsCC BY 4.0
dc.subject.otherbacteroidetes
dc.subject.otherCRISPR
dc.subject.otherFlavobacterium columnare
dc.subject.othergenome
dc.subject.otherprophage
dc.titleProphages and Past Prophage-Host Interactions Revealed by CRISPR Spacer Content in a Fish Pathogen
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202012086964
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineBiologisten vuorovaikutusten huippututkimusyksikköfi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineCentre of Excellence in Biological Interactions Researchen
dc.contributor.oppiaineNanoscience Centeren
dc.contributor.oppiaineCell and Molecular Biologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn2076-2607
dc.relation.numberinseries12
dc.relation.volume8
dc.type.versionpublishedVersion
dc.rights.copyright© 2020 by the authors. Licensee MDPI, Basel, Switzerland
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber314939
dc.subject.ysoperimä
dc.subject.ysokalataudit
dc.subject.ysobakteerit
dc.subject.ysobakteriofagit
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p8862
jyx.subject.urihttp://www.yso.fi/onto/yso/p46
jyx.subject.urihttp://www.yso.fi/onto/yso/p1749
jyx.subject.urihttp://www.yso.fi/onto/yso/p25303
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.3390/microorganisms8121919
dc.relation.funderResearch Council of Finlanden
dc.relation.funderSuomen Akatemiafi
jyx.fundingprogramAcademy Project, AoFen
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundinginformationThis work was supported by the Academy of Finland grants to L.-R.S. (#314939) and E.L. (#321985) and the Jane and Aatos Erkko Foundation. This work resulted from the BONUS Flavophage project supported by BONUS (Art 185), funded jointly by the European Union (EU) and the Academy of Finland.
dc.type.okmA1


Aineistoon kuuluvat tiedostot

Thumbnail

Aineisto kuuluu seuraaviin kokoelmiin

Näytä suppeat kuvailutiedot

CC BY 4.0
Ellei muuten mainita, aineiston lisenssi on CC BY 4.0