Prevalence of genetically similar Flavobacterium columnare phages across aquaculture environments reveals a strong potential for pathogen control
Runtuvuori‐Salmela, A., Kunttu, H. M. T., Laanto, E., Almeida, G. M. F., Mäkelä, K., Middelboe, M., & Sundberg, L‐R. (2022). Prevalence of genetically similar Flavobacterium columnare phages across aquaculture environments reveals a strong potential for pathogen control. Environmental Microbiology, 24(5), Article 2404-2420. https://doi.org/10.1111/1462-2920.15901
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Environmental MicrobiologyAuthors
Date
2022Discipline
Akvaattiset tieteetNanoscience CenterSolu- ja molekyylibiologiaBiologisten vuorovaikutusten huippututkimusyksikköResurssiviisausyhteisöAquatic SciencesNanoscience CenterCell and Molecular BiologyCentre of Excellence in Biological Interactions ResearchSchool of Resource WisdomCopyright
© 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd
Intensive aquaculture conditions expose fish to bacterial infections, leading to significant financial losses, extensive antibiotic use and risk of antibiotic resistance in target bacteria. Flavobacterium columnare causes columnaris disease in aquaculture worldwide. To develop a bacteriophage-based control of columnaris disease, we isolated and characterized 126 F. columnare strains and 63 phages against F. columnare from Finland and Sweden in 2017. Bacterial isolates were virulent on rainbow trout (Oncorhynchus mykiss) and fell into four previously described genetic groups A, C, E and G, with genetic groups C and E being the most virulent. Phage host range studied against a collection of 227 bacterial isolates (from 2013-2017) demonstrated modular infection patterns based on host genetic group. Phages infected contemporary and previously isolated bacterial hosts, but bacteria isolated most recently were generally resistant to previously isolated phages. Despite large differences in geographical origin, isolation year or host range of the phages, whole genome sequencing of 56 phages showed high level of genetic similarity to previously isolated F. columnare phages (Ficleduovirus, Myoviridae). Altogether, this phage collection demonstrates a potential for use in phage therapy.
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Wiley-BlackwellISSN Search the Publication Forum
1462-2912Keywords
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https://converis.jyu.fi/converis/portal/detail/Publication/103942676
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Research Council of FinlandFunding program(s)
Academy Project, AoFAdditional information about funding
This work resulted from the BONUS FLAVOPHAGE project supported by BONUS (Art 185), funded jointly by the EU and Academy of Finland and Innovation Fund Denmark. We also acknowledge funding from Academy of Finland (grants #314939 and #321985) and Jane and Aatos Erkko Foundation.License
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