The effects of growth medium on Flavobacterium columnare colony morphology and its phage resistance mechanisms
Authors
Date
2019Copyright
This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.
The freshwater bacterium Flavobacterium columnare is known to exhibit different morphologies when cultured in different media with bacteriophage. This could influence the type of phage-resistance mechanisms the bacteria use, such as CRISPR-Cas system or alterations which reduce bacteriophage adsorption. In this study the different morphologies which arise in different media when treated with bacteriophage was investigated to identify their favoured phage-resistance mechanism. The bacteria were screened for acquisition of new CRISPR spaces and tested for changes in phage adsorption. Results show that the growth medium itself influences the bacterial colony morphology, likely due to the difference in nutrients available. When exposed to bacteriophage, a small proportion of bacteria in HTC medium acquired new CRISPR spacers, however, the preferred phage-resistance mechanism appears to be something other than CRISPR-Cas overall, in SHIEH and HTC. Improved understanding of bacterial resistance mechanisms is useful towards the development of bacteriophage therapies.
...


Keywords
Metadata
Show full item recordCollections
- Pro gradu -tutkielmat [24521]
Related items
Showing items with similar title or keywords.
-
Cooperation between Different CRISPR-Cas Types Enables Adaptation in an RNA-Targeting System
Hoikkala, Ville; Ravantti, Janne; Díez-Villaseñor, César; Tiirola, Marja; Conrad, Rachel A.; McBride, Mark J.; Moineau, Sylvain; Sundberg, Lotta-Riina (American Society for Microbiology, 2021)CRISPR-Cas immune systems adapt to new threats by acquiring new spacers from invading nucleic acids such as phage genomes. However, some CRISPR-Cas loci lack genes necessary for spacer acquisition despite variation in ... -
Interactions between Rainbow Trout Eyed Eggs and Flavobacterium spp. Using a Bath Challenge Model : Preliminary Evaluation of Bacteriophages as Pathogen Control Agents
Donati, Valentina L.; Dalsgaard, Inger; Runtuvuori-Salmela, Anniina; Kunttu, Heidi; Jørgensen, Johanna; Castillo, Daniel; Sundberg, Lotta-Riina; Middelboe, Mathias; Madsen, Lone (MDPI AG, 2021)The microbial community surrounding fish eyed eggs can harbor pathogenic bacteria. In this study we focused on rainbow trout (Oncorhynchus mykiss) eyed eggs and the potential of bacteriophages against the pathogenic bacteria ... -
Prophages and Past Prophage-Host Interactions Revealed by CRISPR Spacer Content in a Fish Pathogen
Laanto, Elina; Ravantti, Janne J.; Sundberg, Lotta-Riina (MDPI AG, 2020)The role of prophages in the evolution, diversification, or virulence of the fish pathogen Flavobacterium columnare has not been studied thus far. Here, we describe a functional spontaneously inducing prophage fF4 from the ... -
Comparison of Delivery Methods in Phage Therapy against Flavobacterium columnare Infections in Rainbow Trout
Kunttu, Heidi M. T.; Runtuvuori-Salmela, Anniina; Middelboe, Mathias; Clark, Jason; Sundberg, Lotta-Riina (MDPI AG, 2021)Viruses of bacteria, bacteriophages, specifically infect their bacterial hosts with minimal effects on the surrounding microbiota. They have the potential to be used in the prevention and treatment of bacterial infections, ... -
Memoirs of a fish pathogen: how CRISPR-Cas captures phage encounters in Flavobacterium columnare
Hoikkala, Ville (2020)Bacteria are in constant interaction with their viruses, bacteriophages (phages). To prevent or abort phage infections, a variety of defence mechanisms have evolved. CRISPR-Cas, the only known adaptive bacterial immune ...