Show simple item record

dc.contributor.authorKarvinen, Sira
dc.contributor.authorSievänen, Tero
dc.contributor.authorKarppinen, Jari E.
dc.contributor.authorHautasaari, Pekka
dc.contributor.authorBart, Geneviève
dc.contributor.authorSamoylenko, Anatoliy
dc.contributor.authorVainio, Seppo J.
dc.contributor.authorAhtiainen, Juha P.
dc.contributor.authorLaakkonen, Eija K.
dc.contributor.authorKujala, Urho M.
dc.date.accessioned2020-08-19T07:25:47Z
dc.date.available2020-08-19T07:25:47Z
dc.date.issued2020
dc.identifier.citationKarvinen, S., Sievänen, T., Karppinen, J. E., Hautasaari, P., Bart, G., Samoylenko, A., Vainio, S. J., Ahtiainen, J. P., Laakkonen, E. K., & Kujala, U. M. (2020). MicroRNAs in Extracellular Vesicles in Sweat Change in Response to Endurance Exercise. <i>Frontiers in Physiology</i>, <i>11</i>, Article 676. <a href="https://doi.org/10.3389/fphys.2020.00676" target="_blank">https://doi.org/10.3389/fphys.2020.00676</a>
dc.identifier.otherCONVID_41771114
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/71453
dc.description.abstractBackground: To date, microRNAs (miRs) carried in extracellular vesicles (EVs) in response to exercise have been studied in blood but not in non-invasively collectable body fluids. In the present study, we examined whether six exercise–responsive miRs, miRs-21, -26, -126, -146, -221, and -222, respond to acute endurance exercise stimuli of different intensities in sweat. Methods: We investigated the response of miRs isolated from sweat and serum EVs to three endurance exercise protocols: (1) maximal aerobic capacity (VO2max), (2) anaerobic threshold (AnaT), and (3) aerobic threshold (AerT) tests. Sauna bathing was used as a control test to induce sweating through increased body temperature in the absence of exercise. All protocols were performed by the same subjects (n = 8, three males and five females). The occurrence of different miR carriers in sweat and serum was investigated via EV markers (CD9, CD63, and TSG101), an miR-carrier protein (AGO2), and an HDL-particle marker (APOA1) with Western blot. Correlations between miRs in sweat and serum (post-sample) were examined. Results: Of the studied miR carrier markers, sweat EV fractions expressed CD63 and, very weakly, APOA1, while the serum EV fraction expressed all the studied markers. In sweat EVs, miR-21 level increased after AerT and miR-26 after all the endurance exercise tests compared with the Sauna (p < 0.050). miR-146 after AnaT correlated to sweat and serum EV samples (r = 0.881, p = 0.004). Conclusion: Our preliminary study is the first to show that, in addition to serum, sweat EVs carry miRs. Interestingly, we observed that miRs-21 and -26 in sweat EVs respond to endurance exercise of different intensities. Our data further confirmed that miR responses to endurance exercise in sweat and serum were triggered by exercise and not by increased body temperature. Our results highlight that sweat possesses a unique miR carrier content that should be taken into account when planning analyses from sweat as a substitute for serum.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.language.isoeng
dc.publisherFrontiers Media
dc.relation.ispartofseriesFrontiers in Physiology
dc.rightsCC BY 4.0
dc.subject.othercirculating microRNA
dc.subject.othersauna
dc.subject.otherserum
dc.subject.otheracute exercise response
dc.subject.otherleukocyte
dc.titleMicroRNAs in Extracellular Vesicles in Sweat Change in Response to Endurance Exercise
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202008195587
dc.contributor.laitosLiikuntatieteellinen tiedekuntafi
dc.contributor.laitosFaculty of Sport and Health Sciencesen
dc.contributor.oppiaineBiomekaniikkafi
dc.contributor.oppiaineLiikuntafysiologiafi
dc.contributor.oppiaineValmennus- ja testausoppifi
dc.contributor.oppiaineLiikuntalääketiedefi
dc.contributor.oppiaineGerontologia ja kansanterveysfi
dc.contributor.oppiaineGerontologian tutkimuskeskusfi
dc.contributor.oppiaineHyvinvoinnin tutkimuksen yhteisöfi
dc.contributor.oppiaineBiomechanicsen
dc.contributor.oppiaineExercise Physiologyen
dc.contributor.oppiaineScience of Sport Coaching and Fitness Testingen
dc.contributor.oppiaineSports and Exercise Medicineen
dc.contributor.oppiaineGerontology and Public Healthen
dc.contributor.oppiaineGerontology Research Centeren
dc.contributor.oppiaineSchool of Wellbeingen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn1664-042X
dc.relation.volume11
dc.type.versionpublishedVersion
dc.rights.copyright© 2020 the Authors
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber5802/31/2016
dc.relation.grantnumber309504
dc.subject.ysofysiologiset vaikutukset
dc.subject.ysosaunominen
dc.subject.ysobiomarkkerit
dc.subject.ysokestävyysharjoittelu
dc.subject.ysoharjoitusvaste
dc.subject.ysohiki
dc.subject.ysovalkosolut
dc.subject.ysomikro-RNA
dc.subject.ysoseerumi
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p11511
jyx.subject.urihttp://www.yso.fi/onto/yso/p11049
jyx.subject.urihttp://www.yso.fi/onto/yso/p12288
jyx.subject.urihttp://www.yso.fi/onto/yso/p7676
jyx.subject.urihttp://www.yso.fi/onto/yso/p24755
jyx.subject.urihttp://www.yso.fi/onto/yso/p8170
jyx.subject.urihttp://www.yso.fi/onto/yso/p18721
jyx.subject.urihttp://www.yso.fi/onto/yso/p27218
jyx.subject.urihttp://www.yso.fi/onto/yso/p4127
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.3389/fphys.2020.00676
dc.relation.funderTEKESen
dc.relation.funderResearch Council of Finlanden
dc.relation.funderTEKESfi
dc.relation.funderSuomen Akatemiafi
jyx.fundingprogramOthers, TEKESen
jyx.fundingprogramAcademy Research Fellow, AoFen
jyx.fundingprogramMuut, TEKESfi
jyx.fundingprogramAkatemiatutkija, SAfi
jyx.fundinginformationThis project was funded by Business Finland (grant 5802/31/2016 to UK) and the Academy of Finland (grant 309504 to EL). GB, AS, and SV were supported by Academy of Finland (grant 315030 and 307533 to SV), FET OPEN MindGAP (grant 829040), and the Sigrid Jusélius Foundation (SV).
dc.type.okmA1


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

CC BY 4.0
Except where otherwise noted, this item's license is described as CC BY 4.0