MicroRNAs in Extracellular Vesicles in Sweat Change in Response to Endurance Exercise
Karvinen, S., Sievänen, T., Karppinen, J. E., Hautasaari, P., Bart, G., Samoylenko, A., Vainio, S. J., Ahtiainen, J. P., Laakkonen, E. K., & Kujala, U. M. (2020). MicroRNAs in Extracellular Vesicles in Sweat Change in Response to Endurance Exercise. Frontiers in Physiology, 11, Article 676. https://doi.org/10.3389/fphys.2020.00676
Julkaistu sarjassa
Frontiers in PhysiologyTekijät
Päivämäärä
2020Oppiaine
BiomekaniikkaLiikuntafysiologiaValmennus- ja testausoppiLiikuntalääketiedeGerontologia ja kansanterveysGerontologian tutkimuskeskusHyvinvoinnin tutkimuksen yhteisöBiomechanicsExercise PhysiologyScience of Sport Coaching and Fitness TestingSports and Exercise MedicineGerontology and Public HealthGerontology Research CenterSchool of WellbeingTekijänoikeudet
© 2020 the Authors
Background: To date, microRNAs (miRs) carried in extracellular vesicles (EVs) in response to exercise have been studied in blood but not in non-invasively collectable body fluids. In the present study, we examined whether six exercise–responsive miRs, miRs-21, -26, -126, -146, -221, and -222, respond to acute endurance exercise stimuli of different intensities in sweat.
Methods: We investigated the response of miRs isolated from sweat and serum EVs to three endurance exercise protocols: (1) maximal aerobic capacity (VO2max), (2) anaerobic threshold (AnaT), and (3) aerobic threshold (AerT) tests. Sauna bathing was used as a control test to induce sweating through increased body temperature in the absence of exercise. All protocols were performed by the same subjects (n = 8, three males and five females). The occurrence of different miR carriers in sweat and serum was investigated via EV markers (CD9, CD63, and TSG101), an miR-carrier protein (AGO2), and an HDL-particle marker (APOA1) with Western blot. Correlations between miRs in sweat and serum (post-sample) were examined.
Results: Of the studied miR carrier markers, sweat EV fractions expressed CD63 and, very weakly, APOA1, while the serum EV fraction expressed all the studied markers. In sweat EVs, miR-21 level increased after AerT and miR-26 after all the endurance exercise tests compared with the Sauna (p < 0.050). miR-146 after AnaT correlated to sweat and serum EV samples (r = 0.881, p = 0.004).
Conclusion: Our preliminary study is the first to show that, in addition to serum, sweat EVs carry miRs. Interestingly, we observed that miRs-21 and -26 in sweat EVs respond to endurance exercise of different intensities. Our data further confirmed that miR responses to endurance exercise in sweat and serum were triggered by exercise and not by increased body temperature. Our results highlight that sweat possesses a unique miR carrier content that should be taken into account when planning analyses from sweat as a substitute for serum.
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Julkaisija
Frontiers MediaISSN Hae Julkaisufoorumista
1664-042XAsiasanat
Julkaisu tutkimustietojärjestelmässä
https://converis.jyu.fi/converis/portal/detail/Publication/41771114
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Suomen AkatemiaRahoitusohjelmat(t)
Muut, TEKES; Akatemiatutkija, SALisätietoja rahoituksesta
This project was funded by Business Finland (grant 5802/31/2016 to UK) and the Academy of Finland (grant 309504 to EL). GB, AS, and SV were supported by Academy of Finland (grant 315030 and 307533 to SV), FET OPEN MindGAP (grant 829040), and the Sigrid Jusélius Foundation (SV).Lisenssi
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