Retene, pyrene and phenanthrene cause distinct molecular-level changes in the cardiac tissue of rainbow trout (Oncorhynchus mykiss) larvae, Part 2 – Proteomics and metabolomics
Rigaud, C., Eriksson, A., Rokka, A., Skaugen, M., Lihavainen, J., Keinänen, M., Lehtivuori, H., & Vehniäinen, E.-R. (2020). Retene, pyrene and phenanthrene cause distinct molecular-level changes in the cardiac tissue of rainbow trout (Oncorhynchus mykiss) larvae, Part 2 – Proteomics and metabolomics. Science of the Total Environment, 746, Article 141161. https://doi.org/10.1016/j.scitotenv.2020.141161
Julkaistu sarjassa
Science of the Total EnvironmentTekijät
Päivämäärä
2020Tekijänoikeudet
© 2020 Elsevier B.V. All rights reserved.
Polycyclic aromatic hydrocarbons (PAHs) are global contaminants of concern. Despite several decades of research, their mechanisms of toxicity are not very well understood. Early life stages of fish are particularly sensitive with the developing cardiac tissue being a main target of PAHs toxicity. The mechanisms of cardiotoxicity of the three widespread model polycyclic aromatic hydrocarbons (PAHs) retene, pyrene and phenanthrene were explored in rainbow trout (Oncorhyncus mykiss) early life stages. Newly hatched larvae were exposed to sublethal doses of each individual PAH causing no detectable morphometric alterations. Changes in the cardiac proteome and metabolome were assessed after 7 or 14 days of exposure to each PAH. Phase I and II enzymes regulated by the aryl hydrocarbon receptor were significantly induced by all PAHs, with retene being the most potent compound. Retene significantly altered the level of several proteins involved in key cardiac functions such as muscle contraction, cellular tight junctions or calcium homeostasis. Those findings were quite consistent with previous reports regarding the effects of retene on the cardiac transcriptome. Significant changes in proteins linked to iron and heme metabolism were observed following exposure to pyrene. While phenanthrene also altered the levels of several proteins in the cardiac tissue, no clear mechanisms or pathways could be highlighted. Due to high variability between samples, very few significant changes were detected in the cardiac metabolome overall. Slight but significant changes were still observed for pyrene and phenanthrene, suggesting possible effects on several energetic or signaling pathways. This study shows that early exposure to different PAHs can alter the expression of key proteins involved in the cardiac function, which could potentially affect negatively the fitness of the larvae and later of the juvenile fish.
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Julkaisija
Elsevier BVISSN Hae Julkaisufoorumista
0048-9697Asiasanat
Julkaisu tutkimustietojärjestelmässä
https://converis.jyu.fi/converis/portal/detail/Publication/41655002
Metadata
Näytä kaikki kuvailutiedotKokoelmat
Rahoittaja(t)
Suomen AkatemiaRahoitusohjelmat(t)
Akatemiatutkijan tutkimuskulut, SA; Akatemiatutkija, SALisätietoja rahoituksesta
This study was funded by the Academy of Finland (projects 285296, 294066 and 319284 to Eeva-Riikka Vehniäinen).Lisenssi
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Retene, pyrene and phenanthrene cause distinct molecular-level changes in the cardiac tissue of rainbow trout (Oncorhynchus mykiss) larvae, part 1 – Transcriptomics
Rigaud, Cyril; Eriksson, Andreas; Krasnov, Aleksei; Wincent, Emma; Pakkanen, Hannu; Lehtivuori, Heli; Ihalainen, Janne; Vehniäinen, Eeva-Riikka (Elsevier BV, 2020)Polycyclic aromatic hydrocarbons (PAHs) are contaminants of concern that impact every sphere of the environment. Despite several decades of research, their mechanisms of toxicity are still poorly understood. This study ... -
Polycyclic aromatic hydrocarbons phenanthrene and retene modify the action potential via multiple ion currents in rainbow trout Oncorhynchus mykiss cardiac myocytes
Vehniäinen, Eeva-Riikka; Haverinen, Jaakko; Vornanen, Matti (John Wiley & Sons, Inc., 2019)Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants in aqueous environments. They affect cardiovascular development and function in fishes. The 3‐ring PAH phenanthrene has recently been shown to impair ... -
Changes in cardiac proteome and metabolome following exposure to the PAHs retene and fluoranthene and their mixture in developing rainbow trout alevins
Eriksson, Andreas N.M.; Rigaud, Cyril; Rokka, Anne; Skaugen, Morten; Lihavainen, Jenna H.; Vehniäinen, Eeva-Riikka (Elsevier BV, 2022)Exposure to polycyclic aromatic hydrocarbons (PAHs) is known to affect developing organisms. Utilization of different omics-based technologies and approaches could therefore provide a base for the discovery of novel ... -
Exposure to retene, fluoranthene, and their binary mixture causes distinct transcriptomic and apical outcomes in rainbow trout (Oncorhynchus mykiss) yolk sac alevins
Eriksson, Andreas N.M.; Rigaud, Cyril; Krasnov, Aleksei; Wincent, Emma; Vehniäinen, Eeva-Riikka (Elsevier, 2022)Polycyclic aromatic hydrocarbons (PAHs) are widely spread environmental contaminants which affect developing organisms. It is known that improper activation of the aryl hydrocarbon receptor (AhR) by some PAHs contributes ... -
Endogenous AhR agonist FICZ accumulates in rainbow trout (Oncorhynchus mykiss) alevins exposed to a mixture of two PAHs, retene and fluoranthene
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