Retene, pyrene and phenanthrene cause distinct molecular-level changes in the cardiac tissue of rainbow trout (Oncorhynchus mykiss) larvae, part 1 – Transcriptomics
Rigaud, C., Eriksson, A., Krasnov, A., Wincent, E., Pakkanen, H., Lehtivuori, H., Ihalainen, J., & Vehniäinen, E.-R. (2020). Retene, pyrene and phenanthrene cause distinct molecular-level changes in the cardiac tissue of rainbow trout (Oncorhynchus mykiss) larvae, part 1 – Transcriptomics. Science of the Total Environment, 745, 141031. https://doi.org/10.1016/j.scitotenv.2020.141031
Published inScience of the Total Environment
DisciplineSoveltava kemiaNanoscience CenterYmpäristötiedeFysiikkaSolu- ja molekyylibiologiaApplied ChemistryNanoscience CenterEnvironmental SciencePhysicsCell and Molecular Biology
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Polycyclic aromatic hydrocarbons (PAHs) are contaminants of concern that impact every sphere of the environment. Despite several decades of research, their mechanisms of toxicity are still poorly understood. This study explores the mechanisms of cardiotoxicity of the three widespread model PAHs retene, pyrene and phenanthrene in the rainbow trout (Oncorhynchus mykiss) early life stages. Newly hatched larvae were exposed to each individual compound at sublethal doses causing no significant increase in the prevalence of deformities. Changes in the cardiac transcriptome were assessed after 1, 3, 7 and 14 days of exposure using custom Salmo salar microarrays. The highest number of differentially expressed genes was observed after 1 or 3 days of exposure, and retene was the most potent compound in that regard. Over-representation analyses suggested that genes related to cardiac ion channels, calcium homeostasis and muscle contraction (actin binding, troponin and myosin complexes) were especially targeted by retene. Pyrene was also able to alter similar myosin-related genes, but at a different timing and in an opposite direction, suggesting compound-specific mechanisms of toxicity. Pyrene and to a lesser extent phenanthrene were altering key genes linked to the respiratory electron transport chain and to oxygen and iron metabolism. Overall, phenanthrene was not very potent in inducing changes in the cardiac transcriptome despite being apparently metabolized at a slower rate than retene and pyrene. The present study shows that exposure to different PAHs during the first few days of the swim-up stage can alter the expression of key genes involved into the cardiac development and function, which could potentially affect negatively the fitness of the larvae in the long term. ...
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Related funder(s)Academy of Finland
Funding program(s)Research costs of Academy Research Fellow, AoF; Research post as Academy Research Fellow, AoF
Additional information about fundingThe present study was funded by the Academy of Finland (projects 285296, 294066 and 319284 to Eeva -Riikka Vehniäinen). The authors are grateful to Mervi Koistinen, Terhi Rahkonen, Jaakko Litmanen as well as the personnel of the Konnevesi Research Station for their technical assistance. We also thank Gerrit Timmerhaus for his precious help during the preparation of the microarrays.
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