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Aptamer-Embedded DNA Origami Cage for Detecting (Glycated) Hemoglobin with a Surface Plasmon Resonance Sensor

dc.contributor.authorDuanghathaipornsuk, Surachet
dc.contributor.authorShen, Boxuan
dc.contributor.authorCameron, Brent D.
dc.contributor.authorIjäs, Heini
dc.contributor.authorLinko, Veikko
dc.contributor.authorKostiainen, Mauri A.
dc.contributor.authorKim, Dong-Shik
dc.date.accessioned2020-06-23T11:57:35Z
dc.date.available2020-06-23T11:57:35Z
dc.date.issued2020
dc.identifier.citationDuanghathaipornsuk, S., Shen, B., Cameron, B. D., Ijäs, H., Linko, V., Kostiainen, M. A., & Kim, D.-S. (2020). Aptamer-Embedded DNA Origami Cage for Detecting (Glycated) Hemoglobin with a Surface Plasmon Resonance Sensor. <i>Materials Letters</i>, <i>275</i>, Article 128141. <a href="https://doi.org/10.1016/j.matlet.2020.128141" target="_blank">https://doi.org/10.1016/j.matlet.2020.128141</a>
dc.identifier.otherCONVID_35991122
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/70228
dc.description.abstractDNA origami-based cages functionalized with aptamer motifs, were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofseriesMaterials Letters
dc.rightsCC BY-NC-ND 4.0
dc.subject.otherDNA origami
dc.subject.otherglycated hemoglobin
dc.subject.otheraptamer
dc.subject.othersurface plasmon resonance sensor
dc.subject.otherdissociation constant
dc.titleAptamer-Embedded DNA Origami Cage for Detecting (Glycated) Hemoglobin with a Surface Plasmon Resonance Sensor
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202006234420
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn0167-577X
dc.relation.volume275
dc.type.versionacceptedVersion
dc.rights.copyright© 2020 Elsevier
dc.rights.accesslevelopenAccessfi
dc.subject.ysoDNA
dc.subject.ysonanorakenteet
dc.subject.ysohemoglobiini
dc.subject.ysoplasmonit
dc.subject.ysobiosensorit
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p7690
jyx.subject.urihttp://www.yso.fi/onto/yso/p25315
jyx.subject.urihttp://www.yso.fi/onto/yso/p21415
jyx.subject.urihttp://www.yso.fi/onto/yso/p38679
jyx.subject.urihttp://www.yso.fi/onto/yso/p22008
dc.rights.urlhttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.relation.doi10.1016/j.matlet.2020.128141
jyx.fundinginformationThe authors acknowledge a Fulbright-Aalto University Distinguished Chair Award (2017-2018) granted to D.S.K, and the financial support from Academy of Finland (grant numbers 286845, 308578, 303804, 267497), Jane and Aatos Erkko Foundation and Sigrid Jusélius Foundation are gratefully acknowledged. We also acknowledge the provision of facilities and technical support by Aalto University at OtaNano - Nanomicroscopy Center (Aalto-NMC).
dc.type.okmA1


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