Aptamer-Embedded DNA Origami Cage for Detecting (Glycated) Hemoglobin with a Surface Plasmon Resonance Sensor

Duanghathaipornsuk, Surachet; Shen, Boxuan; Cameron, Brent D.; Ijäs, Heini; Linko, Veikko; Kostiainen, Mauri A.; Kim,   Dong-Shik

doi:10.1016/j.matlet.2020.128141

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Duanghathaipornsuk, S., Shen, B., Cameron, B. D., Ijäs, H., Linko, V., Kostiainen, M. A., & Kim, D.-S. (2020). Aptamer-Embedded DNA Origami Cage for Detecting (Glycated) Hemoglobin with a Surface Plasmon Resonance Sensor. Materials Letters, 275, Article 128141. https://doi.org/10.1016/j.matlet.2020.128141

Julkaistu sarjassa

Materials Letters

Tekijät

Duanghathaipornsuk, Surachet |

Shen, Boxuan |

Cameron, Brent D. |

Ijäs, Heini |

Linko, Veikko |

Kostiainen, Mauri A. |

Kim, Dong-Shik

Päivämäärä

2020

Oppiaine

Nanoscience Center Nanoscience Center

Tekijänoikeudet

DNA origami-based cages functionalized with aptamer motifs, were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.

Julkaisija

Elsevier

ISSN Hae Julkaisufoorumista

0167-577X

Lisätietoja rahoituksesta

The authors acknowledge a Fulbright-Aalto University Distinguished Chair Award (2017-2018) granted to D.S.K, and the financial support from Academy of Finland (grant numbers 286845, 308578, 303804, 267497), Jane and Aatos Erkko Foundation and Sigrid Jusélius Foundation are gratefully acknowledged. We also acknowledge the provision of facilities and technical support by Aalto University at OtaNano ... showmore

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