Aptamer-Embedded DNA Origami Cage for Detecting (Glycated) Hemoglobin with a Surface Plasmon Resonance Sensor
Duanghathaipornsuk, S., Shen, B., Cameron, B. D., Ijäs, H., Linko, V., Kostiainen, M. A., & Kim, D.-S. (2020). Aptamer-Embedded DNA Origami Cage for Detecting (Glycated) Hemoglobin with a Surface Plasmon Resonance Sensor. Materials Letters, 275, Article 128141. https://doi.org/10.1016/j.matlet.2020.128141
Published inMaterials Letters
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© 2020 Elsevier
DNA origami-based cages functionalized with aptamer motifs, were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.
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Additional information about fundingThe authors acknowledge a Fulbright-Aalto University Distinguished Chair Award (2017-2018) granted to D.S.K, and the financial support from Academy of Finland (grant numbers 286845, 308578, 303804, 267497), Jane and Aatos Erkko Foundation and Sigrid Jusélius Foundation are gratefully acknowledged. We also acknowledge the provision of facilities and technical support by Aalto University at OtaNano - Nanomicroscopy Center (Aalto-NMC). ...
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