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dc.contributor.authorRuotsalainen, Pilvi
dc.contributor.authorGiven, Cindy
dc.contributor.authorPenttinen, Reetta
dc.contributor.authorJalasvuori, Matti
dc.date.accessioned2020-06-09T04:05:05Z
dc.date.available2020-06-09T04:05:05Z
dc.date.issued2020
dc.identifier.citationRuotsalainen, P., Given, C., Penttinen, R., & Jalasvuori, M. (2020). Beta-Lactam Sensitive Bacteria Can Acquire ESBL-Resistance via Conjugation after Long-Term Exposure to Lethal Antibiotic Concentration. <i>Antibiotics</i>, <i>9</i>(6), Article 296. <a href="https://doi.org/10.3390/antibiotics9060296" target="_blank">https://doi.org/10.3390/antibiotics9060296</a>
dc.identifier.otherCONVID_35885251
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/69784
dc.description.abstractBeta-lactams are commonly used antibiotics that prevent cell-wall biosynthesis. Beta-lactam sensitive bacteria can acquire conjugative resistance elements and hence become resistant even after being exposed to lethal (above minimum inhibitory) antibiotic concentrations. Here we show that neither the length of antibiotic exposure (1 to 16 h) nor the beta-lactam type (penam or cephem) have a major impact on the rescue of sensitive bacteria. We demonstrate that an evolutionary rescue can occur between different clinically relevant bacterial species (Klebsiella pneumoniae and Escherichia coli) by plasmids that are commonly associated with extended-spectrum beta-lactamase (ESBL) positive hospital isolates. As such, it is possible that this resistance dynamic may play a role in failing antibiotic therapies in those cases where resistant bacteria may readily migrate into the proximity of sensitive pathogens. Furthermore, we engineered a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) -plasmid to encode a guiding CRISPR-RNA against the migrating ESBL-plasmid. By introducing this plasmid into the sensitive bacterium, the frequency of the evolutionarily rescued bacteria decreased by several orders of magnitude. As such, engineering pathogens during antibiotic treatment may provide ways to prevent ESBL-plasmid dispersal and hence resistance evolution.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofseriesAntibiotics
dc.rightsCC BY 4.0
dc.subject.otherantibiotic resistance
dc.subject.otherextended-spectrum beta-lactamase
dc.subject.otherevolutionary rescue
dc.subject.otherconjugative plasmid
dc.titleBeta-Lactam Sensitive Bacteria Can Acquire ESBL-Resistance via Conjugation after Long-Term Exposure to Lethal Antibiotic Concentration
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202006094041
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineEkologia ja evoluutiobiologiafi
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineBiologisten vuorovaikutusten huippututkimusyksikköfi
dc.contributor.oppiaineEcology and Evolutionary Biologyen
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineCentre of Excellence in Biological Interactions Researchen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn2079-6382
dc.relation.numberinseries6
dc.relation.volume9
dc.type.versionpublishedVersion
dc.rights.copyright© 2020 by the authors. Licensee MDPI, Basel, Switzerland
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber297049
dc.subject.ysobakteerit
dc.subject.ysoantibiootit
dc.subject.ysoplasmidit
dc.subject.ysoKlebsiella-bakteerit
dc.subject.ysokolibakteerit
dc.subject.ysohorisontaalinen geeninsiirto
dc.subject.ysoantibioottiresistenssi
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p1749
jyx.subject.urihttp://www.yso.fi/onto/yso/p10820
jyx.subject.urihttp://www.yso.fi/onto/yso/p27720
jyx.subject.urihttp://www.yso.fi/onto/yso/p38358
jyx.subject.urihttp://www.yso.fi/onto/yso/p18582
jyx.subject.urihttp://www.yso.fi/onto/yso/p29551
jyx.subject.urihttp://www.yso.fi/onto/yso/p29640
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.3390/antibiotics9060296
dc.relation.funderResearch Council of Finlanden
dc.relation.funderSuomen Akatemiafi
jyx.fundingprogramAcademy Research Fellow, AoFen
jyx.fundingprogramAkatemiatutkija, SAfi
jyx.fundinginformationThis research was funded by the Academy of Finland (grants no.252411 and no. 297049 to MJ and no.322204 to RP), Emil Aaltonen Foundation and Jane and Aatos Erkko Foundation.
dc.type.okmA1


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