Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery
Saari, Heikki; Turunen, Tiia; Lõhmus, Andres; Turunen, Mikko; Jalasvuori, Matti; Butcher, Sarah J.; Ylä-Herttuala, Seppo; Viitala, Tapani; Cerullo, Vincenzo; Siljander, Pia R. M.; Yliperttula, Marjo (2020). Extracellular vesicles provide a capsid-free vector for oncolytic adenoviral DNA delivery. Journal of Extracellular Vesicles, 9 (1), 1747206. DOI: 10.1080/20013078.2020.1747206
Published inJournal of Extracellular Vesicles
© 2020 The Authors
Extracellular vesicles (EVs) have been showcased as auspicious candidates for delivering therapeutic cargo, including oncolytic viruses for cancer treatment. Delivery of oncolytic viruses in EVs could provide considerable advantages, hiding the viruses from the immune system and providing alternative entry pathways into cancer cells. Here we describe the formation and viral cargo of EVs secreted by cancer cells infected with an oncolytic adenovirus (IEVs, infected cell-derived EVs) as a function of time after infection. IEVs were secreted already before the lytic release of virions and their structure resembled normally secreted EVs, suggesting that they were not just apoptotic fragments of infected cells. IEVs were able to carry the viral genome and induce infection in other cancer cells. As such, the role of EVs in the life cycle of adenoviruses may be an important part of a successful infection and may also be harnessed for cancer- and gene therapy.