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dc.contributor.authorTossavainen, Helena
dc.contributor.authorSalovaara, Santeri
dc.contributor.authorHellman, Maarit
dc.contributor.authorIhalin, Riikka
dc.contributor.authorPermi, Perttu
dc.date.accessioned2020-03-20T08:04:22Z
dc.date.available2020-03-20T08:04:22Z
dc.date.issued2020
dc.identifier.citationTossavainen, H., Salovaara, S., Hellman, M., Ihalin, R., & Permi, P. (2020). Dispersion from Cα or NH : 4D experiments for backbone resonance assignment of intrinsically disordered proteins. <i>Journal of Biomolecular NMR</i>, <i>74</i>(2-3), 147-159. <a href="https://doi.org/10.1007/s10858-020-00299-w" target="_blank">https://doi.org/10.1007/s10858-020-00299-w</a>
dc.identifier.otherCONVID_34178494
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/68258
dc.description.abstractResonance assignment of intrinsically disordered proteins is remarkably challenging due to scant chemical shift dispersion arising from conformational heterogeneity. The challenge is even greater if repeating segments are present in the amino acid sequence. To forward unambiguous resonance assignment of intrinsically disordered proteins, we present iHACANCO, HACACON and (HACA)CONCAHA, three Hα-detected 4D experiments with Cα as an additional dimension. In addition, we present (HACA)CON(CA)NH and (HACA)N(CA)CONH, new 4D Hα-start, HN-detect experiments which have two NH dimensions to enhance peak dispersion in a sequential walk through C', NH and HN, and provide more accurate NH/HN chemical shifts than those that can be obtained from a crowded 1H, 15N-HSQC spectrum. Application of these 4D experiments is demonstrated using BilRI (165 aa), an outer-membrane intrinsically disordered protein from the opportunistic oral pathogen Aggregatibacter actinomycetemcomitans. BilRI amino acid sequence encompasses three very similar repeats with a 13-residue identical stretch in two of them.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofseriesJournal of Biomolecular NMR
dc.rightsCC BY 4.0
dc.subject.otherAggregatibacter actinomycetemcomitans
dc.subject.otherBilRI
dc.subject.otherIDP
dc.subject.otherintrinsically disordered protein
dc.subject.otherresonance assignment
dc.titleDispersion from Cα or NH : 4D experiments for backbone resonance assignment of intrinsically disordered proteins
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202003202484
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosKemian laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.laitosDepartment of Chemistryen
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.description.reviewstatuspeerReviewed
dc.format.pagerange147-159
dc.relation.issn0925-2738
dc.relation.numberinseries2-3
dc.relation.volume74
dc.type.versionpublishedVersion
dc.rights.copyright© The Author(s) 2020
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber288235
dc.subject.ysoresonanssi
dc.subject.ysoproteiinit
dc.subject.ysoNMR-spektroskopia
dc.subject.ysobakteerit
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p5834
jyx.subject.urihttp://www.yso.fi/onto/yso/p4332
jyx.subject.urihttp://www.yso.fi/onto/yso/p26254
jyx.subject.urihttp://www.yso.fi/onto/yso/p1749
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1007/s10858-020-00299-w
dc.relation.funderSuomen Akatemiafi
dc.relation.funderAcademy of Finlanden
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundingprogramAcademy Project, AoFen
jyx.fundinginformationOpen access funding provided by University of Jyväskylä (JYU). This work is supported by the grants from the Academy of Finland (Number 288235 to PP, 265609, 272960 and 303781 to RI).


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