1H, 13C, and 15N NMR chemical shift assignment of the complex formed by the first EPEC EspF repeat and N-WASP GTPase binding domain
Karjalainen, M., Hellman, M., Tossavainen, H., & Permi, P. (2021). 1H, 13C, and 15N NMR chemical shift assignment of the complex formed by the first EPEC EspF repeat and N-WASP GTPase binding domain. Biomolecular NMR Assignments, 15(1), 213-217. https://doi.org/10.1007/s12104-021-10008-9
Published inBiomolecular NMR Assignments
© Authors, 2021
LEE-encoded effector EspF (EspF) is an effector protein part of enteropathogenic Escherichia coli’s (EPEC’s) arsenal for intestinal infection. This intrinsically disordered protein contains three highly conserved repeats which together compose over half of the protein’s complete amino acid sequence. EPEC uses EspF to hijack host proteins in order to promote infection. In the attack EspF is translocated, together with other effector proteins, to host cell via type III secretion system. Inside host EspF stimulates actin polymerization by interacting with Neural Wiskott-Aldrich syndrome protein (N-WASP), a regulator in actin polymerization machinery. It is presumed that EspF acts by disrupting the autoinhibitory state of N-WASP GTPase binding domain. In this NMR spectroscopy study, we report the 1H, 13C, and 15N resonance assignments for the complex formed by the first 47-residue repeat of EspF and N-WASP GTPase binding domain. These near-complete resonance assignments provide the basis for further studies which aim to characterize structure, interactions, and dynamics between these two proteins in solution. ...
ISSN Search the Publication Forum1874-2718
Publication in research information system
MetadataShow full item record
Related funder(s)Academy of Finland
Funding program(s)Academy Project, AoF
Additional information about fundingThis work is supported by the grant from the Academy of Finland (Number 288235 to Perttu Permi). Open Access funding provided by University of Jyväskylä (JYU).
Showing items with similar title or keywords.
HACANCOi : a new Hα-detected experiment for backbone resonance assignment of intrinsically disordered proteins Karjalainen, Mikael; Tossavainen, Helena; Hellman, Maarit; Permi, Perttu (Springer, 2020)Unidirectional coherence transfer is highly efficient in intrinsically disordered proteins (IDPs). Their elevated ps-ns timescale dynamics ensures long transverse (T2) relaxation times allowing sophisticated coherence ...
Dispersion from Cα or NH : 4D experiments for backbone resonance assignment of intrinsically disordered proteins Tossavainen, Helena; Salovaara, Santeri; Hellman, Maarit; Ihalin, Riikka; Permi, Perttu (Springer, 2020)Resonance assignment of intrinsically disordered proteins is remarkably challenging due to scant chemical shift dispersion arising from conformational heterogeneity. The challenge is even greater if repeating segments are ...
1H, 13C and 15N NMR chemical shift assignments of cAMP-regulated phosphoprotein-19 and -16 (ARPP-19 and ARPP-16) Thapa, Chandan J.; Haataja, Tatu; Pentikäinen, Ulla; Permi, Perttu (Springer, 2020)Protein Phosphatase 2A, PP2A, the principal Serine/threonine phosphatase, has major roles in broad range of signaling pathways that include regulation of cell cycle, cell proliferation and neuronal signaling. The loss of ...
The Interaction Mechanism of Intrinsically Disordered PP2A Inhibitor Proteins ARPP-16 and ARPP-19 With PP2A Thapa, Chandan; Roivas, Pekka; Haataja, Tatu; Permi, Perttu; Pentikäinen, Ulla (Frontiers Media SA, 2021)Protein phosphatase 2A (PP2A) activity is critical for maintaining normal physiological cellular functions. PP2A is inhibited by endogenous inhibitor proteins in several pathological conditions including cancer. A PP2A ...
Sequential assignment of the intrinsically disordered protein bacterial interleukin receptor 1 with nuclear magnetic resonance spectroscopy Salovaara, Santeri (2018)Significance of intrinsic disorder in biological systems, nuclear magnetism and different methods for its use in protein structure determination have been reviewed. These methods include the use of different detection ...